Volkan Kilinc, Ryoma Hayakawa, Yusuke Yamauchi, Yutaka Wakayama, Jonathan P. Hill
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引用次数: 0
摘要
将数字数据项编码为合成 DNA 链,然后进行选择性数据检索的方法已经得到证实。然而,这些最初以生物为导向的过程仍然很慢,而且没有得到优化。这里研究的 DNA 场效应晶体管(DNA-FET)是一种可能的随机存取存储器(RAM)设备,可用于简单、选择性和快速的 ssDNA 片段检索,用作数据池识别器。DNA-FET 基于一个共平面金门全有机晶体管,该晶体管附加有短单链 DNA (ssDNA) 探针,探针上带有阻断分子,可防止部分杂交,并实现对短 ssDNA(最长 45 nt)近乎完美的选择性。对结合了 DNA 纳米孔结构的新型活性层的转导率进行检查后发现,结合位点的可及性得到了提高。这反过来又促进了鉴别性杂交,特别是从由九种不同序列(至少有一个核苷酸差异)组成的竞争性浓缩 ssDNA 背景池中物理检索短长 ssDNA。DNA-FET 在毫伏范围内快速运行(9 分钟),检测限低(亚微摩尔),选择性高,可重复使用。考虑到其简单明了的概念、近乎无差错的识别能力和假定的出色可扩展性,本文所述的 DNA-FET 有潜力成为在 DNA 数据存储过程中进一步探索先进 RAM 技术的基础。
Nanoporous Dna Field Effect Transistor with Potential for Random-Access Memory Applications: A Selectivity Performance Evaluation
Methods to encode digital data items as strands of synthetic DNA followed by selective data retrieval have been demonstrated. However, these initially bio-oriented processes remain slow and not optimized. DNA field-effect transistor (DNA-FET) is studied here as a possible random-access memory (RAM) device for simple, selective and rapid ssDNA fragment retrieval used as data pool identifier. The DNA-FET is based on a co-planar Au-gated fully organic transistor appended with short single-stranded DNA (ssDNA) probes bearing a blocking molecule to prevent partial hybridization and achieve near perfect selectivity for short length ssDNA (up to 45 nt). Examination of transconductance of the novel active layer incorporating a DNA nanopore architecture reveals enhanced binding site accessibility. This, in turn, facilitates discriminatory hybridization, particularly in the physical retrieval of short-length ssDNA from a competitive, concentrated ssDNA background pool consisting of nine different sequences, with at least one nucleotide difference. The DNA-FET exhibits rapid operation (9 min) in the millivolt range, low detection limit (sub-femtomolar), high selectivity and reusability. Considering the straightforward concept, near error-free identification capacity and hypothetically outstanding scalability, the DNA-FET described here has potential as a foundation for further exploration of advanced RAM technology in the DNA data storage process.