侏儒霓虹鱼(Melanotaenia praecox)--一种有可能成为新的 Acanthomorpha 模式鱼的小型刺辐鱼:II.建立基因工程显微注射程序

IF 2 3区 生物学 Q2 ANATOMY & MORPHOLOGY Developmental Dynamics Pub Date : 2024-02-05 DOI:10.1002/dvdy.698
Kazuhide Miyamoto, Gembu Abe, Koichi Kawakami, Koji Tamura, Satoshi Ansai
{"title":"侏儒霓虹鱼(Melanotaenia praecox)--一种有可能成为新的 Acanthomorpha 模式鱼的小型刺辐鱼:II.建立基因工程显微注射程序","authors":"Kazuhide Miyamoto,&nbsp;Gembu Abe,&nbsp;Koichi Kawakami,&nbsp;Koji Tamura,&nbsp;Satoshi Ansai","doi":"10.1002/dvdy.698","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Rainbowfish is a clade of colorful freshwater fish. <i>Melanotaenia praecox</i> is a small rainbowfish species with biological characteristics that make it potentially useful as an experimental model species. We anticipate that <i>M. praecox</i> could become a new model used in various fields, such as ecology, evolution, and developmental biology. However, few previous studies have described experimental set-ups needed to understand the molecular and genetic mechanisms within this species.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>We describe detailed procedures for genetic engineering in the rainbowfish <i>M. praecox</i>. By using these procedures, we successfully demonstrated CRISPR/Cas-mediated knockout and <i>Tol2</i> transposon-mediated transgenesis in this species. Regarding the CRISPR/Cas system, we disrupted the <i>tyrosinase</i> gene and then showed that injected embryos lacked pigmentation over much of their body. We also demonstrated that a <i>Tol2</i> construct, including a GFP gene driven by a ubiquitous promoter, was efficiently integrated into the genome of <i>M. praecox</i> embryos.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>The establishment of procedures for genetic engineering in <i>M. praecox</i> enables investigation of the genetic mechanisms behind a broad range of biological phenomena in this species. Thus, we suggest that <i>M. praecox</i> can be used as a new model species in various experimental biology fields.</p>\n </section>\n </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"253 9","pages":"815-828"},"PeriodicalIF":2.0000,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.698","citationCount":"0","resultStr":"{\"title\":\"The dwarf neon rainbowfish Melanotaenia praecox, a small spiny-rayed fish with potential as a new Acanthomorpha model fish: II. Establishment of a microinjection procedure for genetic engineering\",\"authors\":\"Kazuhide Miyamoto,&nbsp;Gembu Abe,&nbsp;Koichi Kawakami,&nbsp;Koji Tamura,&nbsp;Satoshi Ansai\",\"doi\":\"10.1002/dvdy.698\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Rainbowfish is a clade of colorful freshwater fish. <i>Melanotaenia praecox</i> is a small rainbowfish species with biological characteristics that make it potentially useful as an experimental model species. We anticipate that <i>M. praecox</i> could become a new model used in various fields, such as ecology, evolution, and developmental biology. However, few previous studies have described experimental set-ups needed to understand the molecular and genetic mechanisms within this species.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>We describe detailed procedures for genetic engineering in the rainbowfish <i>M. praecox</i>. By using these procedures, we successfully demonstrated CRISPR/Cas-mediated knockout and <i>Tol2</i> transposon-mediated transgenesis in this species. Regarding the CRISPR/Cas system, we disrupted the <i>tyrosinase</i> gene and then showed that injected embryos lacked pigmentation over much of their body. We also demonstrated that a <i>Tol2</i> construct, including a GFP gene driven by a ubiquitous promoter, was efficiently integrated into the genome of <i>M. praecox</i> embryos.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>The establishment of procedures for genetic engineering in <i>M. praecox</i> enables investigation of the genetic mechanisms behind a broad range of biological phenomena in this species. Thus, we suggest that <i>M. praecox</i> can be used as a new model species in various experimental biology fields.</p>\\n </section>\\n </div>\",\"PeriodicalId\":11247,\"journal\":{\"name\":\"Developmental Dynamics\",\"volume\":\"253 9\",\"pages\":\"815-828\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-02-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.698\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Developmental Dynamics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/dvdy.698\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ANATOMY & MORPHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental Dynamics","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/dvdy.698","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

彩虹鱼是多彩淡水鱼的一个支系。虹彩鱼(Melanotaenia praecox)是一种小型虹彩鱼,其生物学特性使其有可能成为实验模型鱼种。我们预计,M. praecox 可能成为生态学、进化论和发育生物学等多个领域使用的新模型。然而,以前的研究很少描述了解该物种的分子和遗传机制所需的实验装置。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
The dwarf neon rainbowfish Melanotaenia praecox, a small spiny-rayed fish with potential as a new Acanthomorpha model fish: II. Establishment of a microinjection procedure for genetic engineering

Background

Rainbowfish is a clade of colorful freshwater fish. Melanotaenia praecox is a small rainbowfish species with biological characteristics that make it potentially useful as an experimental model species. We anticipate that M. praecox could become a new model used in various fields, such as ecology, evolution, and developmental biology. However, few previous studies have described experimental set-ups needed to understand the molecular and genetic mechanisms within this species.

Results

We describe detailed procedures for genetic engineering in the rainbowfish M. praecox. By using these procedures, we successfully demonstrated CRISPR/Cas-mediated knockout and Tol2 transposon-mediated transgenesis in this species. Regarding the CRISPR/Cas system, we disrupted the tyrosinase gene and then showed that injected embryos lacked pigmentation over much of their body. We also demonstrated that a Tol2 construct, including a GFP gene driven by a ubiquitous promoter, was efficiently integrated into the genome of M. praecox embryos.

Conclusions

The establishment of procedures for genetic engineering in M. praecox enables investigation of the genetic mechanisms behind a broad range of biological phenomena in this species. Thus, we suggest that M. praecox can be used as a new model species in various experimental biology fields.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Developmental Dynamics
Developmental Dynamics 生物-发育生物学
CiteScore
5.10
自引率
8.00%
发文量
116
审稿时长
3-8 weeks
期刊介绍: Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.
期刊最新文献
Review on pathogenesis and treatment of Alzheimer's disease. Cover Image Issue Information Editorial Editorial highlights
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1