[6]-Shogaol 可诱导小鼠膀胱癌细胞凋亡。

IF 2.5 Q3 CELL BIOLOGY Cellular Physiology and Biochemistry Pub Date : 2024-01-31 DOI:10.33594/000000682
Diana Gabriela Nina Nina, Thaiane Alcarde Robeldo, Almada da Silva, Vitória Shiévila Dos Santos Gonçalves, Ricardo Carneiro Borra, Fernanda de Freitas Anibal
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引用次数: 0

摘要

背景/目的:膀胱癌因其复发和进展特征而被认为是最具侵袭性的肿瘤之一,即使诊断和治疗方法有所改进,近几十年来其死亡率仍未出现下降趋势。因此,有必要寻找和开发更有效、更安全的替代治疗方法。植物化学物质是具有治疗潜力的活性成分的极佳来源。[6]-Shogaol 是一种从生姜根茎中提取的酚类化合物,在多种癌症模型中显示出抗肿瘤作用。然而,文献中并没有关于这些作用在膀胱癌模型中的研究报告。因此,本研究旨在调查[6]-Shogaol 对小鼠膀胱癌尿道上皮细胞(MB49)的体外细胞毒性和促凋亡潜力:方法:在小鼠尿道膀胱癌细胞系(MB49)中评估了[6]-Shogaol相对于非肿瘤小鼠成纤维细胞(L929)对细胞活力(MTT法)、细胞形态(光学显微镜)、增殖过程的改变(克隆形成试验)、氧化应激途径(活性氧水平)和诱导细胞凋亡事件(流式细胞术和高分辨率荧光成像)的细胞毒性作用。结果显示结果表明,[6]-Shogaol 能诱导浓度依赖性细胞毒性效应,损害细胞活力,对 MB49 肿瘤细胞的 50%细胞抑制浓度(IC50)为 146.8 µM,对 L929 非肿瘤成纤维细胞的抑制浓度(IC50)为 236.0 µM。除了抑制和改变集落形成的增殖过程外,它还通过定量分析和观察凋亡表型发现了促凋亡活性,这些事件显然是由诱导核破碎介导的:所提供的数据表明,与 L929 成纤维细胞相比,[6]-Shogaol 在 MB49 细胞中具有更高的浓度依赖性细胞毒性和凋亡诱导潜力。这些结果可能有助于开发治疗膀胱癌的替代疗法。
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[6]-Shogaol Induces Apoptosis of Murine Bladder Cancer Cells.

Background/aims: Bladder cancer is considered one of the most aggressive neoplasms due to its recurrence and progression profile, and even with the improvement in diagnosis and treatment methods, the mortality rate has not shown a declining trend in recent decades. From this perspective, the search and development of more effective and safer therapeutic alternatives are necessary. Phytochemicals are excellent sources of active principles with therapeutic potential. [6]-Shogaol is a phenolic compound extracted from the ginger rhizomes that has shown antitumor effects in a wide variety of cancer models. However, there is no record in the literature of studies reporting these effects in models of bladder cancer. Thus, this study aimed to investigate the in vitro cytotoxic and pro-apoptotic potential of [6]-Shogaol against murine bladder cancer urothelial cells (MB49).

Methods: The cytotoxic effects of [6]-Shogaol on cell viability (MTT method), cell morphology (light microscopy), alteration of proliferative processes (clonogenic assay), oxidative stress pathway (levels of reactive oxygen species) and the induction of apoptotic events (flow cytometry and high-resolution epifluorescence imaging) were evaluated in murine urothelial bladder cancer cell lines (MB49), relative to non-tumor murine fibroblasts (L929).

Results: The results showed that [6]-Shogaol was able to induce concentration-dependent cytotoxic effects, which compromised cell viability, exhibiting an inhibitory concentration of 50% of cells (IC50) of 146.8 µM for MB49 tumor cells and 236.0 µM for L929 non-tumor fibroblasts. In addition to inhibiting and altering the proliferative processes if colony formation, it presented pro-apoptotic activity identified through a quantitative analysis and the observation of apoptotic phenotypes, events apparently mediated by the induction of nuclear fragmentation.

Conclusion: The data presented suggest that [6]-Shogaol has a higher concentration-dependent cytotoxic and apoptosis-inducing potential in MB49 cells than in L929 fibroblasts. These results may contribute to the development of therapeutic alternatives for bladder cancer.

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来源期刊
CiteScore
5.80
自引率
0.00%
发文量
86
审稿时长
1 months
期刊介绍: Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.
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