不同化学脱细胞方案对肾脏心包特性的影响

IF 1.6 4区医学 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS Cardiovascular Engineering and Technology Pub Date : 2024-06-01 Epub Date: 2024-02-12 DOI:10.1007/s13239-024-00712-7

Thirumalai Deepak, Deepak Bajhaiya, Anju R Babu

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引用次数: 0

摘要

目的：本研究旨在使用不同浓度的非离子表面活性剂和阴离子去污剂对黄羊心包组织进行脱细胞处理：方法：方案 A 包括 1%、0.5% 和 0.25%（w/v）十二烷基硫酸钠（SDS）。方案 B 使用 1%、0.5% 和 0.25%（w/v）的 Triton X-100。C 方案包括 0.5% SDS + 0.5% Triton X-100、0.5% + 0.25% 和 0.25% SDS + 0.5% Triton X-100：结果：处理方案 B 在心包组织中留下了少量可计数的细胞，而处理方案 A 和 C 则清除了所有细胞。DNA 定量也表明，B 方案脱细胞后残留的 DNA 最多。用阴离子洗涤剂和非离子表面活性剂的相同组合处理心包组织可保留基质。然而，改变阴离子洗涤剂与非离子表面活性剂的比例会破坏微观结构。方案 A 降低了心包组织的秒模量（p < 0.05）。经过 B 方案处理的心包组织的秒模量和极限拉伸应力与原生组织相匹配。C方案增强了心包组织：完整的细胞外基质和与原生组织相同的生物力学特性需要最佳的化学剂量和组合。

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Impact of the Different Chemical-Based Decellularization Protocols on the Properties of the Caprine Pericardium.

Purpose: This study aims to decellularized caprine pericardium tissue with varied non-ionic surfactant and anionic detergent concentrations.

Methods: Protocol A consists of 1%, 0.5%, and 0.25% (w/v) sodium dodecyl sulphate (SDS). Protocol B uses 1%, 0.5%, and 0.25% (w/v) Triton X-100. Protocol C comprised 0.5% SDS + 0.5% Triton X-100, 0.5% + 0.25%, and 0.25% SDS + 0.5% Triton X-100.

Results: Protocol B left a few countable cells in the pericardium tissue, but treatments A and C removed all cells. DNA quantification also demonstrated that protocol B had the most leftover DNA after decellularization. The pericardium tissue treated with an equal combination of anionic detergent and non-ionic surfactant preserves the matrix. However, changing the anionic detergent-non-ionic surfactant ratio disrupted the microstructure. Protocol A decreased pericardium tissue secant modulus (p < 0.05). Protocol B-treated pericardium tissue matched native tissue secant modulus and ultimate tensile stress. Protocol C strengthened pericardium tissue.

Conclusion: The intact extracellular matrix and biomechanical properties like native tissues require optimal chemical doses and combinations.

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来源期刊

Cardiovascular Engineering and Technology Engineering-Biomedical Engineering

CiteScore

4.00

自引率

0.00%

发文量

期刊介绍： Cardiovascular Engineering and Technology is a journal publishing the spectrum of basic to translational research in all aspects of cardiovascular physiology and medical treatment. It is the forum for academic and industrial investigators to disseminate research that utilizes engineering principles and methods to advance fundamental knowledge and technological solutions related to the cardiovascular system. Manuscripts spanning from subcellular to systems level topics are invited, including but not limited to implantable medical devices, hemodynamics and tissue biomechanics, functional imaging, surgical devices, electrophysiology, tissue engineering and regenerative medicine, diagnostic instruments, transport and delivery of biologics, and sensors. In addition to manuscripts describing the original publication of research, manuscripts reviewing developments in these topics or their state-of-art are also invited.