{"title":"利用基于适配体的生物分析法和多孔硅 SERS 平台定量检测金黄色葡萄球菌","authors":"Divagar Muthukumar, Giorgi Shtenberg","doi":"10.1016/j.jsamd.2024.100690","DOIUrl":null,"url":null,"abstract":"<div><p>The current study presents a highly sensitive and selective surface-enhanced Raman scattering (SERS) based method for detecting <em>Staphylococcus aureus (S. aureus)</em> in food and water samples. The bioassay includes target bacteria recognition by a specific aptamer complex, fast syringe filtration and the assessment of unbound biorecognition complexes using a silver-coated porous silicon SERS platform. Notably, the developed bioassay showed high sensitivity toward <em>S. aureus</em> detection, achieving an impressive detection limit of 2 CFU mL<sup>−1</sup> and a broad linear response of 10<sup>1</sup>–10<sup>6</sup> CFU mL<sup>−1</sup>. Furthermore, the selectivity, regeneration and overall shelf-life were thoroughly evaluated while depicting satisfactory performances. Finally, the practicality of the developed SERS bioassay was elucidated in various samples (<em>i.e.</em>, fish, milk, groundwater and tahini) spiked with different <em>S. aureus</em> concentrations that revealed recovery values of 93–110%. The successful validation of actual samples' analysis emphasizes the platform's reliability, robustness and suitability for practical use, including on-site operation.</p></div>","PeriodicalId":17219,"journal":{"name":"Journal of Science: Advanced Materials and Devices","volume":"9 2","pages":"Article 100690"},"PeriodicalIF":6.7000,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468217924000212/pdfft?md5=db066721ed72d11b567d02a4390db740&pid=1-s2.0-S2468217924000212-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Quantitative detection of Staphylococcus aureus using aptamer-based bioassay coupled with porous Si SERS platform\",\"authors\":\"Divagar Muthukumar, Giorgi Shtenberg\",\"doi\":\"10.1016/j.jsamd.2024.100690\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The current study presents a highly sensitive and selective surface-enhanced Raman scattering (SERS) based method for detecting <em>Staphylococcus aureus (S. aureus)</em> in food and water samples. The bioassay includes target bacteria recognition by a specific aptamer complex, fast syringe filtration and the assessment of unbound biorecognition complexes using a silver-coated porous silicon SERS platform. Notably, the developed bioassay showed high sensitivity toward <em>S. aureus</em> detection, achieving an impressive detection limit of 2 CFU mL<sup>−1</sup> and a broad linear response of 10<sup>1</sup>–10<sup>6</sup> CFU mL<sup>−1</sup>. Furthermore, the selectivity, regeneration and overall shelf-life were thoroughly evaluated while depicting satisfactory performances. Finally, the practicality of the developed SERS bioassay was elucidated in various samples (<em>i.e.</em>, fish, milk, groundwater and tahini) spiked with different <em>S. aureus</em> concentrations that revealed recovery values of 93–110%. The successful validation of actual samples' analysis emphasizes the platform's reliability, robustness and suitability for practical use, including on-site operation.</p></div>\",\"PeriodicalId\":17219,\"journal\":{\"name\":\"Journal of Science: Advanced Materials and Devices\",\"volume\":\"9 2\",\"pages\":\"Article 100690\"},\"PeriodicalIF\":6.7000,\"publicationDate\":\"2024-02-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2468217924000212/pdfft?md5=db066721ed72d11b567d02a4390db740&pid=1-s2.0-S2468217924000212-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Science: Advanced Materials and Devices\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2468217924000212\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Science: Advanced Materials and Devices","FirstCategoryId":"88","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2468217924000212","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
Quantitative detection of Staphylococcus aureus using aptamer-based bioassay coupled with porous Si SERS platform
The current study presents a highly sensitive and selective surface-enhanced Raman scattering (SERS) based method for detecting Staphylococcus aureus (S. aureus) in food and water samples. The bioassay includes target bacteria recognition by a specific aptamer complex, fast syringe filtration and the assessment of unbound biorecognition complexes using a silver-coated porous silicon SERS platform. Notably, the developed bioassay showed high sensitivity toward S. aureus detection, achieving an impressive detection limit of 2 CFU mL−1 and a broad linear response of 101–106 CFU mL−1. Furthermore, the selectivity, regeneration and overall shelf-life were thoroughly evaluated while depicting satisfactory performances. Finally, the practicality of the developed SERS bioassay was elucidated in various samples (i.e., fish, milk, groundwater and tahini) spiked with different S. aureus concentrations that revealed recovery values of 93–110%. The successful validation of actual samples' analysis emphasizes the platform's reliability, robustness and suitability for practical use, including on-site operation.
期刊介绍：
In 1985, the Journal of Science was founded as a platform for publishing national and international research papers across various disciplines, including natural sciences, technology, social sciences, and humanities. Over the years, the journal has experienced remarkable growth in terms of quality, size, and scope. Today, it encompasses a diverse range of publications dedicated to academic research.
Considering the rapid expansion of materials science, we are pleased to introduce the Journal of Science: Advanced Materials and Devices. This new addition to our journal series offers researchers an exciting opportunity to publish their work on all aspects of materials science and technology within the esteemed Journal of Science.
With this development, we aim to revolutionize the way research in materials science is expressed and organized, further strengthening our commitment to promoting outstanding research across various scientific and technological fields.
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