X E Xiao, W Wang, P W Crous, H K Wang, C Jiao, F Huang, Z X Pu, Z R Zhu, H Y Li
{"title":"与中国柑橘枝干病害有关的 Botryosphaeriaceae 物种。","authors":"X E Xiao, W Wang, P W Crous, H K Wang, C Jiao, F Huang, Z X Pu, Z R Zhu, H Y Li","doi":"10.3767/persoonia.2023.47.03","DOIUrl":null,"url":null,"abstract":"<p><p>Citrus is an important and widely cultivated fruit crop in South China. Although the species of fungal diseases of leaves and fruits have been extensively studied, the causal organisms of branch diseases remain poorly known in China. Species of <i>Botryosphaeriaceae</i> are known as important fungal pathogens causing branch diseases on citrus in the USA and Europe. To determine the diversity of <i>Botryosphaeriaceae</i> species associated with citrus branch diseases in China, surveys were conducted in the major citrus-producing areas from 2017 to 2020. Diseased tissues were collected from twigs, branches and trunks with a range of symptoms including cankers, cracking, dieback and gummosis. Based on morphological characteristics and phylogenetic comparison of the DNA sequences of the internal transcribed spacer region (ITS), the translation elongation factor 1-alpha gene (<i>tef1</i>), the β-tubulin gene (<i>tub2</i>) and the DNA-directed RNA polymerase II second largest subunit (<i>rpb2</i>), 111 isolates from nine provinces were identified as 18 species of <i>Botryosphaeriaceae</i>, including <i>Botryosphaeria dothidea</i>, <i>B. fabicerciana</i>, <i>Diplodia seriata</i>, <i>Dothiorella alpina</i>, <i>Do. plurivora</i>, <i>Lasiodiplodia citricola</i>, <i>L. iraniensis</i>, <i>L. microconidia</i>, <i>L. pseudotheobromae</i>, <i>L. theobromae</i>, <i>Neodeightonia subglobosa</i>, <i>Neofusicoccum parvum</i>, and six previously undescribed species, namely <i>Do. citrimurcotticola</i>, <i>L.</i> <i>guilinensis</i>, <i>L. huangyanensis</i>, <i>L. linhaiensis</i>, <i>L. ponkanicola</i> and <i>Sphaeropsis linhaiensis</i> spp. nov. <i>Botryosphaeria dothidea</i> (28.8 %) was the most abundant species, followed by <i>L. pseudotheobromae</i> (23.4 %), which was the most widely distributed species on citrus, occurring in six of the nine provinces sampled. Pathogenicity tests indicated that all 18 species of <i>Botryosphaeriaceae</i> obtained from diseased citrus tissues in this study were pathogenic to the tested <i>Citrus reticulata</i> shoots <i>in vitro</i>, while not all species are pathogenic to the tested Cocktail grapefruit (<i>C. paradisi</i> × <i>C. reticulata</i>) shoots <i>in vivo</i>. In addition, <i>Lasiodiplodia</i> was the most aggressive genus both <i>in vitro</i> and <i>in vivo</i>. This is the first study to identify <i>Botryosphaeriaceae</i> species related to citrus branch diseases in China and the results provide a theoretical basis for the implementation of prevention and control measures. <b>Citation</b>: Xiao XE, Wang W, Crous PW, et al. 2021. Species of Botryosphaeriaceae associated with citrus branch diseases in China. Persoonia 47: 106-135. https://doi.org/10.3767/persoonia.2021.47.03.</p>","PeriodicalId":20014,"journal":{"name":"Persoonia","volume":null,"pages":null},"PeriodicalIF":9.5000,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Species of <i>Botryosphaeriaceae</i> associated with citrus branch diseases in China.\",\"authors\":\"X E Xiao, W Wang, P W Crous, H K Wang, C Jiao, F Huang, Z X Pu, Z R Zhu, H Y Li\",\"doi\":\"10.3767/persoonia.2023.47.03\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Citrus is an important and widely cultivated fruit crop in South China. Although the species of fungal diseases of leaves and fruits have been extensively studied, the causal organisms of branch diseases remain poorly known in China. Species of <i>Botryosphaeriaceae</i> are known as important fungal pathogens causing branch diseases on citrus in the USA and Europe. To determine the diversity of <i>Botryosphaeriaceae</i> species associated with citrus branch diseases in China, surveys were conducted in the major citrus-producing areas from 2017 to 2020. Diseased tissues were collected from twigs, branches and trunks with a range of symptoms including cankers, cracking, dieback and gummosis. Based on morphological characteristics and phylogenetic comparison of the DNA sequences of the internal transcribed spacer region (ITS), the translation elongation factor 1-alpha gene (<i>tef1</i>), the β-tubulin gene (<i>tub2</i>) and the DNA-directed RNA polymerase II second largest subunit (<i>rpb2</i>), 111 isolates from nine provinces were identified as 18 species of <i>Botryosphaeriaceae</i>, including <i>Botryosphaeria dothidea</i>, <i>B. fabicerciana</i>, <i>Diplodia seriata</i>, <i>Dothiorella alpina</i>, <i>Do. plurivora</i>, <i>Lasiodiplodia citricola</i>, <i>L. iraniensis</i>, <i>L. microconidia</i>, <i>L. pseudotheobromae</i>, <i>L. theobromae</i>, <i>Neodeightonia subglobosa</i>, <i>Neofusicoccum parvum</i>, and six previously undescribed species, namely <i>Do. citrimurcotticola</i>, <i>L.</i> <i>guilinensis</i>, <i>L. huangyanensis</i>, <i>L. linhaiensis</i>, <i>L. ponkanicola</i> and <i>Sphaeropsis linhaiensis</i> spp. nov. <i>Botryosphaeria dothidea</i> (28.8 %) was the most abundant species, followed by <i>L. pseudotheobromae</i> (23.4 %), which was the most widely distributed species on citrus, occurring in six of the nine provinces sampled. Pathogenicity tests indicated that all 18 species of <i>Botryosphaeriaceae</i> obtained from diseased citrus tissues in this study were pathogenic to the tested <i>Citrus reticulata</i> shoots <i>in vitro</i>, while not all species are pathogenic to the tested Cocktail grapefruit (<i>C. paradisi</i> × <i>C. reticulata</i>) shoots <i>in vivo</i>. In addition, <i>Lasiodiplodia</i> was the most aggressive genus both <i>in vitro</i> and <i>in vivo</i>. This is the first study to identify <i>Botryosphaeriaceae</i> species related to citrus branch diseases in China and the results provide a theoretical basis for the implementation of prevention and control measures. <b>Citation</b>: Xiao XE, Wang W, Crous PW, et al. 2021. Species of Botryosphaeriaceae associated with citrus branch diseases in China. 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引用次数: 0
摘要
柑橘是华南地区广泛种植的重要水果作物。虽然对柑橘叶片和果实的真菌病害种类进行了广泛研究,但对柑橘枝干病害的病原菌却知之甚少。众所周知,在美国和欧洲,Botryosphaeriaceae 的物种是导致柑橘枝干病害的重要真菌病原体。为确定与中国柑橘枝干病害相关的 Botryosphaeriaceae 物种的多样性,2017 年至 2020 年在柑橘主产区进行了调查。从树枝、枝条和树干上采集了病害组织,病害症状包括腐烂病、开裂病、枯死病和胶冻病。根据形态特征以及内部转录间隔区(ITS)、翻译伸长因子 1-α基因(tef1)、β-微管蛋白基因(tub2)和 DNA 引导的 RNA 聚合酶 II 第二大亚基(rpb2)的 DNA 序列的系统发育比较,来自 9 个省的 111 个分离物被鉴定为 18 种 Botryosphaeriaceae,包括 Botryosphaeria dothidea、B.plurivora、Lasiodiplodia citricola、L. iraniensis、L. microconidia、L. pseudotheobromae、L.thobromae、Neodeightonia subglobosa、Neofusicoccum parvum,以及 6 个以前未曾描述过的种,即 Do. citrimurcotticola、L. guilinensis、L. huangyanensis、L. linhaiensis、L. ponkanicola 和 Sphaeropsis linhaiensis spp.在柑橘上分布最广的物种是 L. pseudotheobromae(23.4%),它在取样的 9 个省中的 6 个省都有分布。致病性测试表明,本研究中从病柑橘组织中获得的所有 18 个 Botryosphaeriaceae 物种在体外均对受测的网纹柑橘嫩枝具有致病性,但并非所有物种在体内均对受测的鸡尾柚(C. paradisi × C. reticulata)嫩枝具有致病性。此外,Lasiodiplodia 在体外和体内都是最具侵袭性的属。这是我国首次鉴定与柑橘枝干病害相关的Botryosphaeriaceae物种,其结果为实施防控措施提供了理论依据。引用:Xiao XE, Wang W, Crous PW, et al.与中国柑橘枝干病害相关的 Botryosphaeriaceae 物种.Persoonia 47: 106-135. https://doi.org/10.3767/persoonia.2021.47.03.
Species of Botryosphaeriaceae associated with citrus branch diseases in China.
Citrus is an important and widely cultivated fruit crop in South China. Although the species of fungal diseases of leaves and fruits have been extensively studied, the causal organisms of branch diseases remain poorly known in China. Species of Botryosphaeriaceae are known as important fungal pathogens causing branch diseases on citrus in the USA and Europe. To determine the diversity of Botryosphaeriaceae species associated with citrus branch diseases in China, surveys were conducted in the major citrus-producing areas from 2017 to 2020. Diseased tissues were collected from twigs, branches and trunks with a range of symptoms including cankers, cracking, dieback and gummosis. Based on morphological characteristics and phylogenetic comparison of the DNA sequences of the internal transcribed spacer region (ITS), the translation elongation factor 1-alpha gene (tef1), the β-tubulin gene (tub2) and the DNA-directed RNA polymerase II second largest subunit (rpb2), 111 isolates from nine provinces were identified as 18 species of Botryosphaeriaceae, including Botryosphaeria dothidea, B. fabicerciana, Diplodia seriata, Dothiorella alpina, Do. plurivora, Lasiodiplodia citricola, L. iraniensis, L. microconidia, L. pseudotheobromae, L. theobromae, Neodeightonia subglobosa, Neofusicoccum parvum, and six previously undescribed species, namely Do. citrimurcotticola, L.guilinensis, L. huangyanensis, L. linhaiensis, L. ponkanicola and Sphaeropsis linhaiensis spp. nov. Botryosphaeria dothidea (28.8 %) was the most abundant species, followed by L. pseudotheobromae (23.4 %), which was the most widely distributed species on citrus, occurring in six of the nine provinces sampled. Pathogenicity tests indicated that all 18 species of Botryosphaeriaceae obtained from diseased citrus tissues in this study were pathogenic to the tested Citrus reticulata shoots in vitro, while not all species are pathogenic to the tested Cocktail grapefruit (C. paradisi × C. reticulata) shoots in vivo. In addition, Lasiodiplodia was the most aggressive genus both in vitro and in vivo. This is the first study to identify Botryosphaeriaceae species related to citrus branch diseases in China and the results provide a theoretical basis for the implementation of prevention and control measures. Citation: Xiao XE, Wang W, Crous PW, et al. 2021. Species of Botryosphaeriaceae associated with citrus branch diseases in China. Persoonia 47: 106-135. https://doi.org/10.3767/persoonia.2021.47.03.
期刊介绍:
Persoonia aspires to publish papers focusing on the molecular systematics and evolution of fungi. Additionally, it seeks to advance fungal taxonomy by employing a polythetic approach to elucidate the genuine phylogeny and relationships within the kingdom Fungi. The journal is dedicated to disseminating high-quality papers that unravel both known and novel fungal taxa at the DNA level. Moreover, it endeavors to provide fresh insights into evolutionary processes and relationships. The scope of papers considered encompasses research articles, along with topical and book reviews.