{"title":"制备叶酸-半胱胺修饰的银纳米粒子,作为有望用于计算机断层扫描成像的造影剂","authors":"Wei Lian, Min Gan","doi":"10.1007/s12257-024-00017-5","DOIUrl":null,"url":null,"abstract":"<p>The present work demonstrates the biosynthesis of silver nanoparticles (AgNPs) using <i>Coffea arabica</i> leaf extract. The AgNPs prepared by green route from <i>C. arabica</i> leaf were characterized through UV–visible, X-ray diffraction, transmission electron microscopy and energy-dispersive electron spectroscopy. Later, the prepared NPs were conjugated with cysteamine–folic acid and utilized as a contrast medium for in vitro targeted imaging of folic acid receptor-expressing malignant cells by computerized tomography (CT). At 80 kVp, the targeted cells exhibited CT values which were two times greater than that of the non-targeted cells. The results were compared with the folic acid-negative cell lines as well as the effective inhibition of folic acid receptor using free folic acid substrate. The outcome of the present study suggests that the fabricated cysteamine–folic acid-conjugated silver nanoparticles could be utilized as a potential contrast agent for molecular CT imaging. This information can be taken into consideration for applying AgNPs in enhancing radiation dose where nanoparticles containing greater X-ray attenuation were applied.</p>","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":"42 1","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Fabrication of folic acid–cysteamine-modified silver nanoparticles as promising contrast agent for computed tomography imaging\",\"authors\":\"Wei Lian, Min Gan\",\"doi\":\"10.1007/s12257-024-00017-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The present work demonstrates the biosynthesis of silver nanoparticles (AgNPs) using <i>Coffea arabica</i> leaf extract. The AgNPs prepared by green route from <i>C. arabica</i> leaf were characterized through UV–visible, X-ray diffraction, transmission electron microscopy and energy-dispersive electron spectroscopy. Later, the prepared NPs were conjugated with cysteamine–folic acid and utilized as a contrast medium for in vitro targeted imaging of folic acid receptor-expressing malignant cells by computerized tomography (CT). At 80 kVp, the targeted cells exhibited CT values which were two times greater than that of the non-targeted cells. The results were compared with the folic acid-negative cell lines as well as the effective inhibition of folic acid receptor using free folic acid substrate. The outcome of the present study suggests that the fabricated cysteamine–folic acid-conjugated silver nanoparticles could be utilized as a potential contrast agent for molecular CT imaging. This information can be taken into consideration for applying AgNPs in enhancing radiation dose where nanoparticles containing greater X-ray attenuation were applied.</p>\",\"PeriodicalId\":8936,\"journal\":{\"name\":\"Biotechnology and Bioprocess Engineering\",\"volume\":\"42 1\",\"pages\":\"\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-02-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotechnology and Bioprocess Engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s12257-024-00017-5\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology and Bioprocess Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s12257-024-00017-5","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Fabrication of folic acid–cysteamine-modified silver nanoparticles as promising contrast agent for computed tomography imaging
The present work demonstrates the biosynthesis of silver nanoparticles (AgNPs) using Coffea arabica leaf extract. The AgNPs prepared by green route from C. arabica leaf were characterized through UV–visible, X-ray diffraction, transmission electron microscopy and energy-dispersive electron spectroscopy. Later, the prepared NPs were conjugated with cysteamine–folic acid and utilized as a contrast medium for in vitro targeted imaging of folic acid receptor-expressing malignant cells by computerized tomography (CT). At 80 kVp, the targeted cells exhibited CT values which were two times greater than that of the non-targeted cells. The results were compared with the folic acid-negative cell lines as well as the effective inhibition of folic acid receptor using free folic acid substrate. The outcome of the present study suggests that the fabricated cysteamine–folic acid-conjugated silver nanoparticles could be utilized as a potential contrast agent for molecular CT imaging. This information can be taken into consideration for applying AgNPs in enhancing radiation dose where nanoparticles containing greater X-ray attenuation were applied.
期刊介绍:
Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.