M. A. Kupryashina, E. P. Vetchinkina, A. S. Abdrakhmanova, E. G. Ponomareva, T. E. Pylaev, V. A. Khanadeev, A. M. Burov
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Optimization of Bioformation of Silver Nanoparticles Using Azospirillum brasilense Cultures
Abstract—
Herein we present the results of optimization of silver nanoparticles biosynthesis using a nonpathogenic soil bacterium Azospirillum brasilense. The conditions and parameters for synthesis of biogenic nanoparticles using A. brasilense vegetative cells, culture liquid, and homogeneous polyphenol oxidase preparations were analyzed. We have revealed the ranging effect of the incubation time, the concentration of silver ions, pH of the reaction mixture, and the concentration of metabolites, towards the production of colloidal silver nanoparticles with specified geometric parameters, high colloidal stability and relatively good monodispersity. The size, shape, and relative yield of electron-dense nanostructures were determined by transmission electron microscopy and dynamic light scattering; extinction spectra were recorded by UV-vis spectrophotometry.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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