压电颗粒色谱法:从批量处理到半连续高通量分离

Powders Pub Date : 2024-02-06 DOI:10.3390/powders3010005
J. Giesler, L. Weirauch, Jorg Thöming, G. Pesch, M. Baune
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引用次数: 0

摘要

开发高选择性分离工艺是当前研究的重点。2016 年,德国科学基金会资助了一项优先计划 SPP 2045 "MehrDimPart--具有技术相关性的细颗粒高特异性多维分馏",旨在开发新的或增强现有的纳米和微米级颗粒分离方法。压电分离器可在微流体设备中实现(生物)微粒的高选择性分离,或在非选择性分离足够的情况下处理大量微粒。最近,分离器的设计旨在将高通量和高选择性结合起来。在此,我们总结了通过频率调制介电泳颗粒色谱(DPC)实现微流控快速色谱分离,进而实现宏流控高通量分离的发展历程。此外,我们还提供了新的实验数据,首次证明了 200 nm 聚苯乙烯颗粒在介电泳高通量分离器中的捕获,该分离器使用印刷电路板替代了昂贵的电极阵列,从而为今后的工作提供了一个起点。
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Dielectrophoretic Particle Chromatography: From Batch Processing to Semi-Continuous High-Throughput Separation
The development of highly selective separation processes is a focus of current research. In 2016, the German Science Foundation funded a priority program SPP 2045 “MehrDimPart—highly specific multidimensional fractionation of fine particles with technical relevance” that aims to develop new or enhance existing approaches for the separation of nano- and micrometer-sized particles. Dielectrophoretic separators achieve highly selective separations of (bio-)particles in microfluidic devices or can handle large quantities when non-selective separation is sufficient. Recently, separator designs were developed that aim to combine a high throughput and high selectivity. Here, we summarize the development from a microfluidic fast chromatographic separation via frequency modulated dielectrophoretic particle chromatography (DPC) toward a macrofluidic high throughput separation. Further, we provide a starting point for future work by providing new experimental data demonstrating for the first time the trapping of 200 nm polystyrene particles in a dielectrophoretic high-throughput separator that uses printed circuit boards as alternatives for expensive electrode arrays.
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