M Padrón, M. Milhes, M. Massot, E. Guichoux, Guizien K.
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Development of a set of SNP markers for population genetics of the sea rose, Pentapora fascialis
A set of single nucleotide polymorphisms (SNP) was developed from the transcriptome of 10 individuals of the sea rose Pentapora fascialis (Cheilostomata) collected in Banyuls-sur-Mer Bay (NW Mediterranean sea). 53,042 putative SNPs were identified and mapped on a de novo assembled transcriptome. A selected set of 320 SNPs with highest coverage and uniquely mapped in the assembled transcriptome was tested using a MassARRAY System on 95 individuals sampled in natural rocks and artificial reefs distributed over a hundred kilometers in the NW Mediterranean (outisde Banyuls-sur-Mer Bay). A total of 177 SNPs were successfully genotyped and found to be polymorphic. Among these, 154 SNPs were in Hardy-Weinberg equilibrium over all samples, with significant linkage disequilibrium in only 21 pairs of SNPs. The newly developed loci will be a valuable tool for population genetics studies of this calcifying bryozoan species whose erect structure makes it an engineering species and a target for conservation plans.
期刊介绍：
Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.
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