染色质结构修饰剂对黑腹果蝇反式异染色质位置效应的影响

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Doklady Biochemistry and Biophysics Pub Date : 2024-02-20 DOI:10.1134/S160767292470073X
A. A. Solodovnikov, S. A. Lavrov, A. S. Shatskikh,  V. A. Gvozdev
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引用次数: 0

摘要

异染色质位置效应表现为转移到异染色质的优染色质基因失活。在染色体重排中,位于重排中新的优染色质-异染色质边界附近的基因(顺式失活),在极少数情况下,正常染色体上与重排染色体的优染色质-异染色质边界同源区域的基因(反式失活)也会失活。In(2)A4 反转能反式灭活位于正常染色体上的 UAS-eGFP 报告基因。我们利用温控 RNA 干扰敲除了一些染色质蛋白,并研究了敲除对报告基因反式失活的影响。我们发现,敲除与关键异染色质蛋白HP1a结合的蛋白Su(var)2-HP2、染色质重塑复合物的亚基SAYP和引入HP1a蛋白识别的H3K9me3组蛋白标记的Eggless组蛋白甲基转移酶(SETDB1)会抑制反式失活。这项工作提出的研究基因敲除对异染色质位置效应影响的方法具有独立的方法学意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Effects of Chromatin Structure Modifiers on the trans-Acting Heterochromatin Position Effect in Drosophila melanogaster

The heterochromatin position effect is manifested in the inactivation of euchromatin genes transferred to heterochromatin. In chromosomal rearrangements, genes located near the new eu-heterochromatin boundary in the rearrangement (cis-inactivation) and, in rare cases, genes of a region of the normal chromosome homologous to the region of the eu-heterochromatin boundary of the chromosome with the rearrangement (trans-inactivation) are subject to inactivation. The In(2)A4 inversion is able to trans-inactivate the UAS-eGFP reporter gene located on the normal chromosome. We knockdown a number of chromatin proteins using temperature-controlled RNA interference and investigated the effect of knockdown on trans-inactivation of the reporter. We found suppression of trans-inactivation by knockdowns of Su(var)2-HP2, a protein that binds to the key heterochromatin protein HP1a, SAYP, a subunit of the chromatin remodelling complex, and Eggless histone methyltransferase (SETDB1), which introduces a H3K9me3 histone mark, recognized by the HP1a protein. The method of studying the effects of gene knockdown on heterochromatin position effects presented in this work is of independent methodological interest.

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来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
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