Redife Aslıhan Uçar, Furkan Demirgül, Ömer Şimşek, Hüseyin Erten
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引用次数: 0
摘要
随着人们对维生素 A 的前体--β-胡萝卜素的健康益处及其着色特性有了更深入的了解,各行各业对这种类胡萝卜素的需求也在不断增加。为了高效、廉价和可持续地满足日益增长的需求,近年来,人们对通过代谢工程策略生产异源β-胡萝卜素的兴趣与日俱增。在这种情况下,尽管亚罗酵母不是β-胡萝卜素的原生生产者,但由于其代谢、生理和基因组特性,它脱颖而出。在利用脂溶性亚罗酵母生产异源β-胡萝卜素的过程中,采用了一系列工程策略,包括生物合成途径工程、形态学工程和发酵工程策略,取得了成功的结果。然而,这些策略之间各有优缺点,也有一些有待改进之处。在这篇综述中,我们深入探讨了已经应用和有可能应用于从溶脂酵母中生产β-胡萝卜素的工程策略,包括它们的优缺点,并进行了相互比较。此外,还提出了未来的展望,以提高利用脂溶性酵母作为细胞工厂生产β-胡萝卜素的潜力。
A comparison of metabolic engineering strategies applied in Yarrowia lipolytica for β-carotene production
With a better understanding of the health benefits of β-carotene, the precursor of vitamin A, as well as its coloring property, the need for this carotenoid has increased in various sectors. In order to meet the increasing demand efficiently, cheaply, and sustainably, interest in heterologous β-carotene production through metabolic engineering strategies has increased in recent years. In this context, although it is not a native producer of β-carotene, Yarrowia lipolytica yeast stands out due to its metabolic, physiological, and genomic properties. Successful results have been obtained by using a series of engineering strategies, including biosynthesis pathway engineering, morphological engineering, and fermentation engineering strategies, in the production of heterologous β-carotene from Y. lipolytica. However, these strategies have various strengths and weaknesses against each other, and there are also some points open to improvement. In this review, the engineering strategies that have been applied and have the potential to be applied for the production of β-carotene from Y. lipolytica have been examined in depth, including their advantages and disadvantages, and compared with each other. Moreover, a future perspective has been presented to increase the potential of using Y. lipolytica yeast as a cell factory in β-carotene production.
期刊介绍:
Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.