Faiza EL Hajli, Mohamed Reda Kachmar, Amine Assouguem, Riaz Ullah, Ahmed Bari, Khalil Hammani, Said Chakir, Rachid Lahlali, Essaïd Ait Barka, Ghizlane Echchgadda
{"title":"蒿草提取物和精油的植物化学分析、体外抗氧化和抗真菌活性","authors":"Faiza EL Hajli, Mohamed Reda Kachmar, Amine Assouguem, Riaz Ullah, Ahmed Bari, Khalil Hammani, Said Chakir, Rachid Lahlali, Essaïd Ait Barka, Ghizlane Echchgadda","doi":"10.1515/chem-2023-0200","DOIUrl":null,"url":null,"abstract":"<jats:italic>Artemisia herba-alba</jats:italic> Asso is an endemic plant from North-East Morocco offering a diverse range of pharmacological, cosmetic, and agro-ecological uses. However, <jats:italic>Artemisia herba-alba</jats:italic> has not been well exploited in the field of plant protection (post-harvest diseases). This is why the main objective of the present study is to evaluate the antifungal and antioxidant activities of extracts or the essential oil of this plant. Antifungal activity was assessed against <jats:italic>Botrytis cinerea</jats:italic>, <jats:italic>Penicillium expansum,</jats:italic> and <jats:italic>Penicillium digitatum</jats:italic> using the agar dilution method for the aqueous extracts and fumigation for the essential oil. Antioxidant activity was assessed using the DPPH scavenging test and the FRAP ferric ion reduction capacity. The chemical composition of the extracts was determined by HPLC and that of the essential oil by GC-MS/MS. In addition, the polyphenol, flavonoid, and tannin content was determined using colorimetric methods. The results of this study showed that the total content of polyphenols (217.60 mg GAE/g E), flavonoids (43.59 mg QE/g E), and tannins (32.58 mg GAE/g E) was significantly higher (<jats:italic>p</jats:italic> ≤ 0.05) in the ethanolic extract than in the aqueous and hexanoic extracts. Moreover, the HPLC analysis of the aqueous extract revealed the presence of six compounds, namely, catechin, gallic acid, hydroxybenzoic acid, syringic acid, caffeic acid, and rutin. In addition, the ethanolic extract was found to contain seven phenolic compounds. On the one hand, 35 compounds were identified in the essential oil of <jats:italic>Artemisia herba-alba</jats:italic>, representing 99.7% of the total. According to this study, the dominant compounds in the essential oil of <jats:italic>Artemisia herba-alba</jats:italic> Asso are camphor (46.57%), endo-borneol (5.65%), eucalyptol (5.64%), and thymol (3.85%). Furthermore, the biological evaluation showed that the extracts and essential oil of <jats:italic>Artemisia herba-alba</jats:italic> have significant antioxidant and antibacterial activities. The findings show that <jats:italic>Artemisia herba-alba</jats:italic> is a plant that can be used as a source of antifungal chemicals to prevent putrefaction of foodstuffs and, more specifically, postharvest diseases.","PeriodicalId":19520,"journal":{"name":"Open Chemistry","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phytochemical analysis, in vitro antioxidant and antifungal activities of extracts and essential oil derived from Artemisia herba-alba Asso\",\"authors\":\"Faiza EL Hajli, Mohamed Reda Kachmar, Amine Assouguem, Riaz Ullah, Ahmed Bari, Khalil Hammani, Said Chakir, Rachid Lahlali, Essaïd Ait Barka, Ghizlane Echchgadda\",\"doi\":\"10.1515/chem-2023-0200\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<jats:italic>Artemisia herba-alba</jats:italic> Asso is an endemic plant from North-East Morocco offering a diverse range of pharmacological, cosmetic, and agro-ecological uses. However, <jats:italic>Artemisia herba-alba</jats:italic> has not been well exploited in the field of plant protection (post-harvest diseases). This is why the main objective of the present study is to evaluate the antifungal and antioxidant activities of extracts or the essential oil of this plant. Antifungal activity was assessed against <jats:italic>Botrytis cinerea</jats:italic>, <jats:italic>Penicillium expansum,</jats:italic> and <jats:italic>Penicillium digitatum</jats:italic> using the agar dilution method for the aqueous extracts and fumigation for the essential oil. Antioxidant activity was assessed using the DPPH scavenging test and the FRAP ferric ion reduction capacity. The chemical composition of the extracts was determined by HPLC and that of the essential oil by GC-MS/MS. In addition, the polyphenol, flavonoid, and tannin content was determined using colorimetric methods. The results of this study showed that the total content of polyphenols (217.60 mg GAE/g E), flavonoids (43.59 mg QE/g E), and tannins (32.58 mg GAE/g E) was significantly higher (<jats:italic>p</jats:italic> ≤ 0.05) in the ethanolic extract than in the aqueous and hexanoic extracts. Moreover, the HPLC analysis of the aqueous extract revealed the presence of six compounds, namely, catechin, gallic acid, hydroxybenzoic acid, syringic acid, caffeic acid, and rutin. In addition, the ethanolic extract was found to contain seven phenolic compounds. On the one hand, 35 compounds were identified in the essential oil of <jats:italic>Artemisia herba-alba</jats:italic>, representing 99.7% of the total. According to this study, the dominant compounds in the essential oil of <jats:italic>Artemisia herba-alba</jats:italic> Asso are camphor (46.57%), endo-borneol (5.65%), eucalyptol (5.64%), and thymol (3.85%). 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Phytochemical analysis, in vitro antioxidant and antifungal activities of extracts and essential oil derived from Artemisia herba-alba Asso
Artemisia herba-alba Asso is an endemic plant from North-East Morocco offering a diverse range of pharmacological, cosmetic, and agro-ecological uses. However, Artemisia herba-alba has not been well exploited in the field of plant protection (post-harvest diseases). This is why the main objective of the present study is to evaluate the antifungal and antioxidant activities of extracts or the essential oil of this plant. Antifungal activity was assessed against Botrytis cinerea, Penicillium expansum, and Penicillium digitatum using the agar dilution method for the aqueous extracts and fumigation for the essential oil. Antioxidant activity was assessed using the DPPH scavenging test and the FRAP ferric ion reduction capacity. The chemical composition of the extracts was determined by HPLC and that of the essential oil by GC-MS/MS. In addition, the polyphenol, flavonoid, and tannin content was determined using colorimetric methods. The results of this study showed that the total content of polyphenols (217.60 mg GAE/g E), flavonoids (43.59 mg QE/g E), and tannins (32.58 mg GAE/g E) was significantly higher (p ≤ 0.05) in the ethanolic extract than in the aqueous and hexanoic extracts. Moreover, the HPLC analysis of the aqueous extract revealed the presence of six compounds, namely, catechin, gallic acid, hydroxybenzoic acid, syringic acid, caffeic acid, and rutin. In addition, the ethanolic extract was found to contain seven phenolic compounds. On the one hand, 35 compounds were identified in the essential oil of Artemisia herba-alba, representing 99.7% of the total. According to this study, the dominant compounds in the essential oil of Artemisia herba-alba Asso are camphor (46.57%), endo-borneol (5.65%), eucalyptol (5.64%), and thymol (3.85%). Furthermore, the biological evaluation showed that the extracts and essential oil of Artemisia herba-alba have significant antioxidant and antibacterial activities. The findings show that Artemisia herba-alba is a plant that can be used as a source of antifungal chemicals to prevent putrefaction of foodstuffs and, more specifically, postharvest diseases.
期刊介绍:
Open Chemistry is a peer-reviewed, open access journal that publishes original research, reviews and short communications in the fields of chemistry in an ongoing way. The central goal is to provide a hub for researchers working across all subjects to present their discoveries, and to be a forum for the discussion of the important issues in the field. The journal is the premier source for cutting edge research in fundamental chemistry and it provides high quality peer review services for its authors across the world. Moreover, it allows for libraries everywhere to avoid subscribing to multiple local publications, and to receive instead all the necessary chemistry research from a single source available to the entire scientific community.