抑制氧化应激诱导的铁蛋白沉积可缓解人类类风湿性关节炎

IF 3.5 3区 医学 Q2 IMMUNOLOGY Journal of Immunology Research Pub Date : 2024-02-26 DOI:10.1155/2024/9943747
Yang Liu, Jiang Liang, Zongge Sha, Changfu Yang
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引用次数: 0

摘要

类风湿性关节炎(RA)是一种慢性自身免疫性疾病,以关节滑膜炎为主要特征。不同疾病的铁蛋白沉积特异性不同,RA 中铁蛋白沉积的机制尚不明确。因此,本文深入观察了 RA 患者和动物模型的铁蛋白沉积机制。本文应用RA患者血浆、肿瘤坏死因子-α诱导的人滑膜成纤维细胞和胶原蛋白诱导的关节炎动物模型,初步探究了嗜铁细胞增多症的治疗作用。对于 RA 患者,ELISA 检测了谷胱甘肽(GSH)、GPX4、Nrf2、Keap-1 和铁蛋白的蛋白表达。在细胞实验中,麦拉宁或铁蛋白-1可调节人滑膜成纤维细胞的侵袭、线粒体膜电位、活性氧(ROS)表达、标志蛋白等。在动物实验中,32只Sprague-Dawley雄性大鼠被随机分为4组,在胶原蛋白诱导的RA模型中饲养14天,并服用依拉斯汀或铁-1 35天。结果显示,RA 患者的 GSH、GPX4、Nrf2 和 Keap-1 的表达量较低,而铁蛋白的表达量较高,且这些蛋白的表达量在疾病缓解后有显著变化。此外,铁氧化失活还会降低细胞的增殖和迁移能力、线粒体膜电位和 ROS。我们意外地发现,激活高铁血红蛋白可有效抑制 CIA 动物足部肿胀,减少关节炎评分、骨质破坏和关节滑膜炎,并降低血浆中炎症因子的高表达。人类疾病表现与动物模型病理之间存在非线性关系。对人类或动物的铁蛋白沉积进行调节可能会产生不同的效果。
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Inhibition of Oxidative Stress-Induced Ferroptosis Can Alleviate Rheumatoid Arthritis in Human
Rheumatoid arthritis (RA) is a chronic autoimmunity illness, mainly featured with synovitis of the joint. The specificity of ferroptosis is disparate in different diseases, and the mechanism of ferroptosis in RA has some uncertainty yet. Therefore, the mechanism of ferroptosis was deeply observed in RA patients and animal models. In this paper, plasma of RA patients, the tumor necrosis factor-alpha-induced human synovial fibroblasts, and an animal model of arthritis induced by collagen were applied to initially inquire about the therapeutic effect of ferroptosis. For the RA patients, ELISA detected protein expression of glutathione (GSH), GPX4, Nrf2, Keap-1, and ferritin. In cell experiments, erastin or fer-1 regulated the invasion of human synovial fibroblast cells, mitochondrial membrane potential, reactive oxygen species (ROS) expression, marker protein, and so on. For the animal experiments, 32 Sprague–Dawley male rats were randomly separated into four groups with a collagen-induced RA model for 14 days and administered with erastin or fer-1 for 35 days. The expressions of GSH, GPX4, Nrf2, and Keap-1 were lower, and the ferritin was higher in RA patients, and the expressions of these proteins varied significantly after disease remission. In addition, ferroptosis inactivation also reduced the proliferation and migration ability, mitochondrial membrane potential, and ROS in cells. We discovered unexpectedly that activation of ferroptosis meaningfully forbore the foot swelling in animals with CIA, reduced arthritis scores, destruction of bone, and articular synovitis, and also decreased the high expression of inflammatory factors in plasma. There is a nonlinear relationship between human disease manifestations and animal model pathology. Ferroptosis regulating in RA for humans or animals may produce different effects.
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来源期刊
CiteScore
6.90
自引率
2.40%
发文量
423
审稿时长
15 weeks
期刊介绍: Journal of Immunology Research is a peer-reviewed, Open Access journal that provides a platform for scientists and clinicians working in different areas of immunology and therapy. The journal publishes research articles, review articles, as well as clinical studies related to classical immunology, molecular immunology, clinical immunology, cancer immunology, transplantation immunology, immune pathology, immunodeficiency, autoimmune diseases, immune disorders, and immunotherapy.
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