Lukas Friedeheim, Sjef Boeren, Irene Sánchez-Andrea, Alfons J M Stams, Diana Z Sousa
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引用次数: 0
摘要
Desulfofundulus kuznetsovii 是肽球菌科的一种嗜热、孢子形成型硫酸盐还原细菌。在本研究中,我们描述了一株新分离的 D. kuznetsovii 菌株 TPOSR,并将其新陈代谢过程与 D. kuznetsovii 17T 型菌株进行了比较。这两株菌株都能在甲醇、乙醇和丙二醇等多种醇类的作用下生长,并能还原硫酸盐。菌株 17T 通过两种途径代谢甲醇,一种涉及依赖钴的甲基转移酶,另一种使用不依赖钴的醇脱氢酶。然而,菌株 TPOSR 与 D. kuznetsovii 菌株 17T 的平均核苷酸相同度为 97%,却缺少菌株 17T 中甲基转移酶操作子中的几个基因。编码具有催化活性的甲基转移酶亚基 B 的基因缺失,表明菌株 TPOSR 只利用醇脱氢酶途径。在钴饥饿期间,两株菌株都能利用甲醇生长,但生长受到影响。由于其甲基转移酶系统受到抑制,菌株 17T 对钴缺乏更为敏感。我们的发现揭示了 D. kuznetsovii 的代谢多样性及其编码一种或两种甲醇转化途径的代谢差异。
Alcohol dehydrogenase system acts as the sole pathway for methanol oxidation in Desulfofundulus kuznetsovii strain TPOSR.
Desulfofundulus kuznetsovii is a thermophilic, spore-forming sulphate-reducing bacterium in the family Peptococcaceae. In this study, we describe a newly isolated strain of D. kuznetsovii, strain TPOSR, and compare its metabolism to the type strain D. kuznetsovii 17T. Both strains grow on a large variety of alcohols, such as methanol, ethanol and propane-diols, coupled to the reduction of sulphate. Strain 17T metabolizes methanol via two routes, one involving a cobalt-dependent methyl transferase and the other using a cobalt-independent alcohol dehydrogenase. However, strain TPOSR, which shares 97% average nucleotide identity with D. kuznetsovii strain 17T, lacks several genes from the methyl transferase operon found in strain 17T. The gene encoding the catalytically active methyl transferase subunit B is missing, indicating that strain TPOSR utilizes the alcohol dehydrogenase pathway exclusively. Both strains grew with methanol during cobalt starvation, but growth was impaired. Strain 17T was more sensitive to cobalt deficiency, due to the repression of its methyl transferase system. Our findings shed light on the metabolic diversity of D. kuznetsovii and their metabolic differences of encoding one or two routes for the conversion of methanol.
期刊介绍:
Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.