Tatsuya Nakayama, Hien Thi Li, Phong Thanh Ngo, Doan Nguyen Minh Tran, Oanh Thi Hoang Nguyen, Phuong Hoai Hoang, Takahiro Yamaguchi, Michio Jinnai, Phuc Do Nguyen, Chinh Van Dang, Yuko Kumeda, Atsushi Hase
{"title":"头孢菌素耐药基因 blaCTX-M-55、blaCTX-M-65 和 blaCMY-2 的获得导致携带 mcr-1 基因的耐大肠菌群对喹诺酮类药物产生耐药性","authors":"Tatsuya Nakayama, Hien Thi Li, Phong Thanh Ngo, Doan Nguyen Minh Tran, Oanh Thi Hoang Nguyen, Phuong Hoai Hoang, Takahiro Yamaguchi, Michio Jinnai, Phuc Do Nguyen, Chinh Van Dang, Yuko Kumeda, Atsushi Hase","doi":"10.1111/jfs.13109","DOIUrl":null,"url":null,"abstract":"<p>Food contamination with plasmid-mediated antibiotic-resistant <i>Escherichia coli</i> has not been thoroughly investigated. This study aimed to clarify the prevalence of colistin-resistant AmpC/extended-spectrum β-lactamase (ESBL)-producing <i>E. coli</i> harbouring <i>mcr</i> (COL-ESBL-EC) and to determine antibiotic resistance by comparison with AmpC/ESBL-producing <i>E. coli</i> (ESBL-EC) and colistin-resistant E. coli harbouring <i>mcr</i> (COL-EC). Sixty chicken meats were tested for COL-ESBL-EC contamination. The result showed that 64 COL-ESBL-EC were isolated from 66.7% of the samples, and compared with ESBL-EC and COL-EC were isolated before. The genotypes of the COL-ESBL-EC showed <i>bla</i><sub>CTX-M-55/TEM</sub>, <i>bla</i><sub>CTX-M-55</sub>, <i>bla</i><sub>CMY-2</sub>, and <i>bla</i><sub>CTX-M-65</sub> were predominant. The COL-ESBL-EC results showed a similar trend for <i>bla</i><sub>CTX-M</sub> identification to that of ESBL-EC. The <i>mcr</i> was investigated, and <i>mcr-1</i> was detected in COL-ESBL-EC and COL-EC. Antibiotic susceptibility testing revealed that many strains of COL-ESBL-EC and ESBL-EC were resistant to quinolones, and fosfomycin (FOS). These results suggest that COL-ESBL-EC has simultaneously acquired AmpC/ESBL-related, quinolone, and FOS resistance genes in COL-EC.</p>","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfs.13109","citationCount":"0","resultStr":"{\"title\":\"Acquisition of cephalosporin resistance genes blaCTX-M-55, blaCTX-M-65, and blaCMY-2 leads to quinolone resistance in colistin-resistant Escherichia coli harboring mcr-1 gene\",\"authors\":\"Tatsuya Nakayama, Hien Thi Li, Phong Thanh Ngo, Doan Nguyen Minh Tran, Oanh Thi Hoang Nguyen, Phuong Hoai Hoang, Takahiro Yamaguchi, Michio Jinnai, Phuc Do Nguyen, Chinh Van Dang, Yuko Kumeda, Atsushi Hase\",\"doi\":\"10.1111/jfs.13109\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Food contamination with plasmid-mediated antibiotic-resistant <i>Escherichia coli</i> has not been thoroughly investigated. This study aimed to clarify the prevalence of colistin-resistant AmpC/extended-spectrum β-lactamase (ESBL)-producing <i>E. coli</i> harbouring <i>mcr</i> (COL-ESBL-EC) and to determine antibiotic resistance by comparison with AmpC/ESBL-producing <i>E. coli</i> (ESBL-EC) and colistin-resistant E. coli harbouring <i>mcr</i> (COL-EC). Sixty chicken meats were tested for COL-ESBL-EC contamination. The result showed that 64 COL-ESBL-EC were isolated from 66.7% of the samples, and compared with ESBL-EC and COL-EC were isolated before. The genotypes of the COL-ESBL-EC showed <i>bla</i><sub>CTX-M-55/TEM</sub>, <i>bla</i><sub>CTX-M-55</sub>, <i>bla</i><sub>CMY-2</sub>, and <i>bla</i><sub>CTX-M-65</sub> were predominant. The COL-ESBL-EC results showed a similar trend for <i>bla</i><sub>CTX-M</sub> identification to that of ESBL-EC. The <i>mcr</i> was investigated, and <i>mcr-1</i> was detected in COL-ESBL-EC and COL-EC. Antibiotic susceptibility testing revealed that many strains of COL-ESBL-EC and ESBL-EC were resistant to quinolones, and fosfomycin (FOS). These results suggest that COL-ESBL-EC has simultaneously acquired AmpC/ESBL-related, quinolone, and FOS resistance genes in COL-EC.</p>\",\"PeriodicalId\":15814,\"journal\":{\"name\":\"Journal of Food Safety\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfs.13109\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Food Safety\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jfs.13109\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Safety","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jfs.13109","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Acquisition of cephalosporin resistance genes blaCTX-M-55, blaCTX-M-65, and blaCMY-2 leads to quinolone resistance in colistin-resistant Escherichia coli harboring mcr-1 gene
Food contamination with plasmid-mediated antibiotic-resistant Escherichia coli has not been thoroughly investigated. This study aimed to clarify the prevalence of colistin-resistant AmpC/extended-spectrum β-lactamase (ESBL)-producing E. coli harbouring mcr (COL-ESBL-EC) and to determine antibiotic resistance by comparison with AmpC/ESBL-producing E. coli (ESBL-EC) and colistin-resistant E. coli harbouring mcr (COL-EC). Sixty chicken meats were tested for COL-ESBL-EC contamination. The result showed that 64 COL-ESBL-EC were isolated from 66.7% of the samples, and compared with ESBL-EC and COL-EC were isolated before. The genotypes of the COL-ESBL-EC showed blaCTX-M-55/TEM, blaCTX-M-55, blaCMY-2, and blaCTX-M-65 were predominant. The COL-ESBL-EC results showed a similar trend for blaCTX-M identification to that of ESBL-EC. The mcr was investigated, and mcr-1 was detected in COL-ESBL-EC and COL-EC. Antibiotic susceptibility testing revealed that many strains of COL-ESBL-EC and ESBL-EC were resistant to quinolones, and fosfomycin (FOS). These results suggest that COL-ESBL-EC has simultaneously acquired AmpC/ESBL-related, quinolone, and FOS resistance genes in COL-EC.
期刊介绍：
The Journal of Food Safety emphasizes mechanistic studies involving inhibition, injury, and metabolism of food poisoning microorganisms, as well as the regulation of growth and toxin production in both model systems and complex food substrates. It also focuses on pathogens which cause food-borne illness, helping readers understand the factors affecting the initial detection of parasites, their development, transmission, and methods of control and destruction.
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