巨噬细胞激活剂GcMAF-RF通过诱导M2-M1巨噬细胞重编程增强卡拉那汉技术的抗肿瘤效果

IF 3.5 3区 医学 Q2 IMMUNOLOGY Journal of Immunology Research Pub Date : 2024-02-29 DOI:10.1155/2024/7484490
Vera S. Ruzanova, Svetlana S. Kirikovich, Evgeniy V. Levites, Anastasia S. Proskurina, Evgeniya V. Dolgova, Genrikh S. Ritter, Yaroslav R. Efremov, Tatyana D. Dubatolova, Alexander V. Sysoev, Danil I. Koleno, Alexandr A. Ostanin, Elena R. Chernykh, Sergey S. Bogachev
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引用次数: 0

摘要

巨噬细胞是具有高度免疫可塑性的免疫细胞,既能发挥促炎活性,也能发挥抗炎活性,还能将其表型重新极化为相反或中性的表型。在这方面,肿瘤相关基质(TAS)的M2巨噬细胞是治疗恶性肿瘤的一个很有前景的治疗靶点。我们使用 FACS 分析法估算了未经处理或根据卡拉纳汉技术结合组特异性巨噬细胞激活剂(GcMAF-RF)处理的完整健康小鼠和患 Lewis 癌小鼠腹膜和 TAS 中巨噬细胞的 CD11b+/Ly-6G+/Ly-6C+ 部分。此外,还评估了不同组实验动物和肿瘤动物体内促炎和抗炎细胞因子 mRNA 的表达模式。研究发现(i) 将完整的小鼠暴露于 GcMAF-RF 会导致 CD11b+/Ly-6C+ 腹膜巨噬细胞的数量增加,同时,腹膜巨噬细胞中细胞因子的表达模式会从 M1/M2 混合表型转变为中性 M0 表型;(ii) 结合使用 Karanahan 技术和 GcMAF-RF 处理,可使 TAS 巨噬细胞发生 M0/M1 极化;(iii) 在肿瘤小鼠中,腹腔巨噬细胞对这种处理的反应与诱导抗炎反应有关,这与 TAS 巨噬细胞的反应相反。
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The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage Reprogramming
Macrophages are the immune cells of high-immunological plasticity, which can exert both pro- and anti-inflammatory activity, as well as repolarize their phenotype to the opposite or neutral one. In this regard, M2 macrophages of the tumor-associated stroma (TAS) are a promising therapeutic target in treating malignant neoplasms. Using FACS assay, we have estimated the CD11b+/Ly-6G+/Ly-6C+ fraction of macrophages from the peritoneum and TAS in intact healthy mice and those with developed Lewis carcinoma, both untreated and treated according to Karanahan technology in combination with group-specific macrophage activator (GcMAF-RF). As well, the pattern of pro- and anti-inflammatory cytokines mRNA expression in different groups of experimental and tumor-bearing animals was assessed. It was found that: (i) exposure of intact mice to GcMAF-RF results in the increased number of CD11b+/Ly-6C+ peritoneal macrophages and, at the same time, the expression pattern of cytokines in peritoneal macrophages switches from that characteristic of the mixed M1/M2 phenotype to that characteristic of the neutral M0 one; (ii) combination of Karanahan technology and GcMAF-RF treatment results in M0/M1 repolarization of TAS macrophages; (iii) in tumor-bearing mice, the response of peritoneal macrophages to such a treatment is associated with the induction of anti-inflammatory reaction, which is opposite to that in TAS macrophages.
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来源期刊
CiteScore
6.90
自引率
2.40%
发文量
423
审稿时长
15 weeks
期刊介绍: Journal of Immunology Research is a peer-reviewed, Open Access journal that provides a platform for scientists and clinicians working in different areas of immunology and therapy. The journal publishes research articles, review articles, as well as clinical studies related to classical immunology, molecular immunology, clinical immunology, cancer immunology, transplantation immunology, immune pathology, immunodeficiency, autoimmune diseases, immune disorders, and immunotherapy.
期刊最新文献
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