Jie-Heng Xu , Xin-Yu Xu , Xing-Yu Huang , Ke-Xin Chen , He Wen , Ming Li , Jin-Song Liu
{"title":"长期禁食诱导雌性日本鹌鹑的基础产热灵活性","authors":"Jie-Heng Xu , Xin-Yu Xu , Xing-Yu Huang , Ke-Xin Chen , He Wen , Ming Li , Jin-Song Liu","doi":"10.1016/j.cbpa.2024.111611","DOIUrl":null,"url":null,"abstract":"<div><p>Male Japanese quails (<em>Coturnix japonica</em>) have been found to exhibit a three-phase metabolic change when subjected to prolonged fasting, during which basal thermogenesis is significantly reduced. A study had shown that there is a significant difference in the body temperature between male and female Japanese quails. However, whether female Japanese quails also show the same characteristic three-phase metabolic change during prolonged fasting and the underlying thermogenesis mechanisms associated with such changes are still unclear. In this study, female Japanese quails were subjected to prolonged starvation, and the body mass, basal metabolic rate (BMR), body temperature, mass of tissues and organs, body fat content, the state-4 respiration (S4R) and cytochrome <em>c</em> oxidase (CCO) activity in the muscle and liver of these birds were measured to determine the status of metabolic changes triggered by the starvation. In addition, the levels of glucose, triglyceride (TG) and uric acid, and thyroid hormones (T<sub>3</sub> and T<sub>4</sub>) in the serum and the mRNA levels of myostatin (<em>MSTN</em>) and avian uncoupling protein (<em>av-UCP</em>) in the muscle were also measured. The results revealed the existence of a three-phase stage similar to that found in male Japanese quails undergoing prolonged starvation. Fasting resulted in significantly lower body mass, BMR, body temperature, tissues masses and most organs masses, as well as S4R and CCO activity in the muscle and liver. The mRNA level of <em>av-UCP</em> decreased during fasting, while that of <em>MSTN</em> increased but only during Phase I and II and decreased significantly during Phase III. Fasting also significantly lowered the T<sub>3</sub> level and the ratio of T<sub>3</sub>/T<sub>4</sub> in the serum. These results indicated that female Japanese quails showed an adaptive response in basal thermogenesis at multiple hierarchical levels, from organismal to biochemical, enzyme and cellular level, gene and endocrine levels and this integrated adjustment could be a part of the adaptation used by female quails to survive long-term fasting.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Long-term fasting induced basal thermogenesis flexibility in female Japanese quails\",\"authors\":\"Jie-Heng Xu , Xin-Yu Xu , Xing-Yu Huang , Ke-Xin Chen , He Wen , Ming Li , Jin-Song Liu\",\"doi\":\"10.1016/j.cbpa.2024.111611\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Male Japanese quails (<em>Coturnix japonica</em>) have been found to exhibit a three-phase metabolic change when subjected to prolonged fasting, during which basal thermogenesis is significantly reduced. A study had shown that there is a significant difference in the body temperature between male and female Japanese quails. However, whether female Japanese quails also show the same characteristic three-phase metabolic change during prolonged fasting and the underlying thermogenesis mechanisms associated with such changes are still unclear. In this study, female Japanese quails were subjected to prolonged starvation, and the body mass, basal metabolic rate (BMR), body temperature, mass of tissues and organs, body fat content, the state-4 respiration (S4R) and cytochrome <em>c</em> oxidase (CCO) activity in the muscle and liver of these birds were measured to determine the status of metabolic changes triggered by the starvation. In addition, the levels of glucose, triglyceride (TG) and uric acid, and thyroid hormones (T<sub>3</sub> and T<sub>4</sub>) in the serum and the mRNA levels of myostatin (<em>MSTN</em>) and avian uncoupling protein (<em>av-UCP</em>) in the muscle were also measured. The results revealed the existence of a three-phase stage similar to that found in male Japanese quails undergoing prolonged starvation. Fasting resulted in significantly lower body mass, BMR, body temperature, tissues masses and most organs masses, as well as S4R and CCO activity in the muscle and liver. The mRNA level of <em>av-UCP</em> decreased during fasting, while that of <em>MSTN</em> increased but only during Phase I and II and decreased significantly during Phase III. Fasting also significantly lowered the T<sub>3</sub> level and the ratio of T<sub>3</sub>/T<sub>4</sub> in the serum. These results indicated that female Japanese quails showed an adaptive response in basal thermogenesis at multiple hierarchical levels, from organismal to biochemical, enzyme and cellular level, gene and endocrine levels and this integrated adjustment could be a part of the adaptation used by female quails to survive long-term fasting.</p></div>\",\"PeriodicalId\":55237,\"journal\":{\"name\":\"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1095643324000382\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1095643324000382","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
研究发现,雄性日本鹌鹑()在长时间禁食后会出现三阶段代谢变化,在此期间基础产热显著降低。一项研究表明,雄性和雌性日本鹌鹑的体温有显著差异。然而,雌性日本鹌鹑在长时间禁食时是否也会出现同样的三阶段代谢变化,以及与这种变化相关的基础产热机制,目前仍不清楚。本研究对雌性日本鹌鹑进行了长时间饥饿,并测定了其体重、基础代谢率(BMR)、体温、组织器官质量、体脂含量、肌肉和肝脏中的4态呼吸(S4R)和细胞色素氧化酶(CCO)活性,以确定饥饿引发的代谢变化状况。此外,还测定了血清中葡萄糖、甘油三酯(TG)和尿酸以及甲状腺激素(T和T)的水平,以及肌肉中肌生长因子()和禽解偶联蛋白()的mRNA水平。研究结果表明,雄性日本鹌鹑在长期饥饿状态下也会出现类似的三阶段。禁食导致体重、基础代谢率、体温、组织质量和大多数器官质量以及肌肉和肝脏中的S4R和CCO活性明显降低。在禁食期间,胰岛素的 mRNA 水平下降,而胰岛素的 mRNA 水平上升,但仅在第一和第二阶段上升,在第三阶段明显下降。禁食还能明显降低血清中的 T 水平和 T/T 比值。这些结果表明,雌性日本鹌鹑在基础产热方面表现出了从机体到生化、酶和细胞水平、基因和内分泌水平等多个层次的适应性反应,这种综合调整可能是雌性鹌鹑适应长期禁食生存的一部分。
Long-term fasting induced basal thermogenesis flexibility in female Japanese quails
Male Japanese quails (Coturnix japonica) have been found to exhibit a three-phase metabolic change when subjected to prolonged fasting, during which basal thermogenesis is significantly reduced. A study had shown that there is a significant difference in the body temperature between male and female Japanese quails. However, whether female Japanese quails also show the same characteristic three-phase metabolic change during prolonged fasting and the underlying thermogenesis mechanisms associated with such changes are still unclear. In this study, female Japanese quails were subjected to prolonged starvation, and the body mass, basal metabolic rate (BMR), body temperature, mass of tissues and organs, body fat content, the state-4 respiration (S4R) and cytochrome c oxidase (CCO) activity in the muscle and liver of these birds were measured to determine the status of metabolic changes triggered by the starvation. In addition, the levels of glucose, triglyceride (TG) and uric acid, and thyroid hormones (T3 and T4) in the serum and the mRNA levels of myostatin (MSTN) and avian uncoupling protein (av-UCP) in the muscle were also measured. The results revealed the existence of a three-phase stage similar to that found in male Japanese quails undergoing prolonged starvation. Fasting resulted in significantly lower body mass, BMR, body temperature, tissues masses and most organs masses, as well as S4R and CCO activity in the muscle and liver. The mRNA level of av-UCP decreased during fasting, while that of MSTN increased but only during Phase I and II and decreased significantly during Phase III. Fasting also significantly lowered the T3 level and the ratio of T3/T4 in the serum. These results indicated that female Japanese quails showed an adaptive response in basal thermogenesis at multiple hierarchical levels, from organismal to biochemical, enzyme and cellular level, gene and endocrine levels and this integrated adjustment could be a part of the adaptation used by female quails to survive long-term fasting.
期刊介绍:
Part A: Molecular & Integrative Physiology of Comparative Biochemistry and Physiology. This journal covers molecular, cellular, integrative, and ecological physiology. Topics include bioenergetics, circulation, development, excretion, ion regulation, endocrinology, neurobiology, nutrition, respiration, and thermal biology. Study on regulatory mechanisms at any level of organization such as signal transduction and cellular interaction and control of behavior are also published.