利用 UPLC-Q-TOF-MS/MS 分析鉴定 Edgeworthia gardneri (Wall.) Meisn.使用 UPLC-Q-TOF-MS/MS 分析。

IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Plant Foods for Human Nutrition Pub Date : 2024-06-01 Epub Date: 2024-03-04 DOI:10.1007/s11130-024-01158-x
Lin Li, Qijun Dai, Bolin Zou, Yanyan Zhang, Xianwen Zhang, Liang Liu
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引用次数: 0

摘要

Edgeworthia gardneri (Wall.) Meisn.属于百合科 Edgeworthia 属,在中国长期以来一直被用作食用和药用植物。枸杞子具有降血糖作用,可用于配制预防和治疗糖尿病的日常饮品。然而,其中的降血糖物质仍然未知。本研究旨在利用超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF-MS/MS)方法,筛选加德纳海藻的α-葡萄糖苷酶抑制剂并分析其化学成分。结果发现,乙酸乙酯馏分(EAF)具有显著的α-葡萄糖苷酶抑制活性和抗氧化活性,但没有显示出α-淀粉酶抑制活性。通过 UPLC-Q-TOF-MS/MS 分析,共鉴定出 67 种化合物;其中 48 种化合物是首次在埃奇沃斯属植物中发现的。此外,五种黄酮类化合物,即异橙皮苷(isoorintin)、仲异橙皮苷(sekoisolaricirinol)、橙皮苷(tiliroside)、菊苷(chrysin)和山柰酚(kempferol)具有α-葡萄糖苷酶抑制活性。芦丁具有α-淀粉酶抑制活性。香豆素的一种 Daphnoretin 具有α-葡萄糖苷酶和α-淀粉酶抑制活性。这些发现丰富了 E. gardneria 的化学库。EAF具有选择性的α-葡萄糖苷酶抑制活性,黄酮类化合物和香豆素可能是EAF的活性成分。E. gardneria对开发多靶点降糖药物具有重要价值。
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Identification of α-Glucosidase-Inhibitors in Edgeworthia gardneri (Wall.) Meisn. Using UPLC-Q-TOF-MS/MS Analysis.

Edgeworthia gardneri (Wall.) Meisn., a member of the genus Edgeworthia in the family Thymelaeaceae, has long been applied as an edible and medicinal plant in China. E. gardneria has a hypoglycemic effect and is used to prepare daily drinks for the prevention and treatment of diabetes. However, the hypoglycemic substances involved remain unknown. The present study aimed to screen the α-glucosidase-inhibitors of E. gardneri and analyze its chemical profile using a ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) method. As a result, the ethyl acetate fraction (EAF) had significant α-glucosidase-inhibitory and antioxidant activities but did not show an α-amylase-inhibitory activity. A total of 67 compounds were identified in the EAF by UPLC-Q-TOF-MS/MS analysis; among them, 48 compounds were first discovered in the genus Edgeworthia. Additionally, five flavonoids, namely, isoorintin, secoisolaricirinol, tiliroside, chrysin, and kaempferol, had α-glucosidase-inhibitory activities. Rutin had a α-amylase-inhibitory activity. Daphnoretin, a kind of coumarin, has α-glucosidase and α-amylase-inhibitory activities. These findings enrich the chemical library of E. gardneria. EAF has a selective α-glucosidase-inhibitory activity, and flavonoids and coumarins may be the active components of EAF. E. gardneria has important value for developing multiple-target hypoglycemic drugs.

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来源期刊
Plant Foods for Human Nutrition
Plant Foods for Human Nutrition 工程技术-食品科技
CiteScore
6.80
自引率
7.50%
发文量
89
审稿时长
12-24 weeks
期刊介绍: Plant Foods for Human Nutrition (previously Qualitas Plantarum) is an international journal that publishes reports of original research and critical reviews concerned with the improvement and evaluation of the nutritional quality of plant foods for humans, as they are influenced by: - Biotechnology (all fields, including molecular biology and genetic engineering) - Food science and technology - Functional, nutraceutical or pharma foods - Other nutrients and non-nutrients inherent in plant foods
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