Zeyu Wu, Jie Song, Yangyang Zhang, Xiyu Yuan, Jin Zhao
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Using a rat caries model, the anti-caries efficacy and safety of AR extract were exhaustively investigated in vivo.</p><p><strong>Results: </strong>AR extract inhibit not only the growth of S. mutans, but also the generation of S. mutans biofilm, hence destroying and eliminating the biofilm. Moreover, AR extract were able to inhibit S. mutans' adherence to saliva-encapsulated hydroxyapatite (HAP). Further, in a rat model of caries, the AR extract is able to greatly reduce the incidence and severity of caries lesions on the smooth surface and pit and fissure of rat molars, while exhibiting excellent biosafety.</p><p><strong>Conclusions: </strong>AR extract exhibit strong antibacterial activity against S. mutans and can lower the incidence and severity of dental cavities in rats. These findings suggest that Arnebia euchroma (Royle) Johnst. could be utilized for the prevention and treatment of dental caries.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10908817/pdf/","citationCount":"0","resultStr":"{\"title\":\"Inhibitory and preventive effects of Arnebia euchroma (Royle) Johnst. root extract on Streptococcus mutans and dental caries in rats.\",\"authors\":\"Zeyu Wu, Jie Song, Yangyang Zhang, Xiyu Yuan, Jin Zhao\",\"doi\":\"10.1038/s41405-024-00196-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Dental caries is one of the prevalent conditions that threaten oral health. Arnebia euchroma (Royle) Johnst. root (AR) extracts exhibit anti-inflammatory, anti-cancer, and antibacterial properties. 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Further, in a rat model of caries, the AR extract is able to greatly reduce the incidence and severity of caries lesions on the smooth surface and pit and fissure of rat molars, while exhibiting excellent biosafety.</p><p><strong>Conclusions: </strong>AR extract exhibit strong antibacterial activity against S. mutans and can lower the incidence and severity of dental cavities in rats. 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引用次数: 0
摘要
背景:龋齿是威胁口腔健康的常见疾病之一。Arnebia euchroma (Royle) Johnst. 根(AR)提取物具有抗炎、抗癌和抗菌特性。本研究旨在探讨 AR 提取物对变异链球菌(S. mutans)UA159 的抗菌影响以及对大鼠的抗龋作用:方法:采用细菌敏感性试验、生物膜敏感性试验和活死人染色技术测定 AR 提取物对变异链球菌及其生物膜的抗菌活性。通过荧光标记细菌,确定了细菌粘附率的影响。利用大鼠龋齿模型,对 AR 提取物的抗龋功效和安全性进行了详尽的体内研究:结果:AR 提取物不仅能抑制变异杆菌的生长,还能抑制变异杆菌生物膜的生成,从而破坏和消除生物膜。此外,AR 提取物还能抑制突变杆状病毒对唾液包裹的羟基磷灰石(HAP)的粘附。此外,在大鼠龋齿模型中,AR 提取物能够大大降低大鼠臼齿光滑表面、凹陷和裂隙中龋齿病变的发生率和严重程度,同时表现出良好的生物安全性:AR提取物对突变杆状病毒具有很强的抗菌活性,能降低大鼠龋齿的发生率和严重程度。这些研究结果表明,Arnebia euchroma (Royle) Johnst.可用于预防和治疗龋齿。
Inhibitory and preventive effects of Arnebia euchroma (Royle) Johnst. root extract on Streptococcus mutans and dental caries in rats.
Background: Dental caries is one of the prevalent conditions that threaten oral health. Arnebia euchroma (Royle) Johnst. root (AR) extracts exhibit anti-inflammatory, anti-cancer, and antibacterial properties. This study was designed to investigate the antibacterial impact of AR extract on Streptococcus mutans (S. mutans) UA159 and the anti-caries effect on rats.
Methods: The antibacterial activity of AR extract against S. mutans and its biofilm was determined using the bacterial sensitivity test, the biofilm sensitivity test, and the live-dead staining technique. By fluorescently tagging bacteria, the influence of bacterial adhesion rate was determined. Using a rat caries model, the anti-caries efficacy and safety of AR extract were exhaustively investigated in vivo.
Results: AR extract inhibit not only the growth of S. mutans, but also the generation of S. mutans biofilm, hence destroying and eliminating the biofilm. Moreover, AR extract were able to inhibit S. mutans' adherence to saliva-encapsulated hydroxyapatite (HAP). Further, in a rat model of caries, the AR extract is able to greatly reduce the incidence and severity of caries lesions on the smooth surface and pit and fissure of rat molars, while exhibiting excellent biosafety.
Conclusions: AR extract exhibit strong antibacterial activity against S. mutans and can lower the incidence and severity of dental cavities in rats. These findings suggest that Arnebia euchroma (Royle) Johnst. could be utilized for the prevention and treatment of dental caries.