{"title":"新型 bHLH 转录因子 CmbHLH110 可加速菊花开花","authors":"Yaoyao Huang, Xiaojuan Xing, Jinyu Jin, Yun Tang, Lian Ding, Aiping Song, Sumei Chen, Fadi Chen, Jiafu Jiang, Weimin Fang","doi":"10.1016/j.hpj.2023.05.022","DOIUrl":null,"url":null,"abstract":"Basic helix–loop–helix (bHLH) transcription factor gene family in plants controls various growth and development aspects; however, the actual roles of these genes in flowering plants are not well known. In this study, a novel bHLH protein CmbHLH110 was found to interact with CmERF110 by and experiments, a chrysanthemum ERF110 homolog that acts as a positive flowering regulator. In addition, was also found to regulate the flowering of chrysanthemums, overexpression of causes chrysanthemums to flower earlier, and suppressed leads to delayed flowering. Furthermore, the loss-of-function mutant of its homologue () had a noticeable late flowering phenotype, and completely complemented the late flowering phenotype of the mutant, whereas heterologous overexpression of in Col-0 caused early flowering. Transcriptome sequencing revealed significant differential expression of flowering-related and circadian clock-related genes in transgenic chrysanthemum. Therefore, we concluded that CmbHLH110, as a novel flowering regulator, could interact with CmERF110 to regulate flowering in chrysanthemum.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7000,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"CmbHLH110, a novel bHLH transcription factor, accelerates flowering in chrysanthemum\",\"authors\":\"Yaoyao Huang, Xiaojuan Xing, Jinyu Jin, Yun Tang, Lian Ding, Aiping Song, Sumei Chen, Fadi Chen, Jiafu Jiang, Weimin Fang\",\"doi\":\"10.1016/j.hpj.2023.05.022\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Basic helix–loop–helix (bHLH) transcription factor gene family in plants controls various growth and development aspects; however, the actual roles of these genes in flowering plants are not well known. In this study, a novel bHLH protein CmbHLH110 was found to interact with CmERF110 by and experiments, a chrysanthemum ERF110 homolog that acts as a positive flowering regulator. In addition, was also found to regulate the flowering of chrysanthemums, overexpression of causes chrysanthemums to flower earlier, and suppressed leads to delayed flowering. Furthermore, the loss-of-function mutant of its homologue () had a noticeable late flowering phenotype, and completely complemented the late flowering phenotype of the mutant, whereas heterologous overexpression of in Col-0 caused early flowering. Transcriptome sequencing revealed significant differential expression of flowering-related and circadian clock-related genes in transgenic chrysanthemum. Therefore, we concluded that CmbHLH110, as a novel flowering regulator, could interact with CmERF110 to regulate flowering in chrysanthemum.\",\"PeriodicalId\":13178,\"journal\":{\"name\":\"Horticultural Plant Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2024-02-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Horticultural Plant Journal\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.hpj.2023.05.022\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticultural Plant Journal","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.hpj.2023.05.022","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
CmbHLH110, a novel bHLH transcription factor, accelerates flowering in chrysanthemum
Basic helix–loop–helix (bHLH) transcription factor gene family in plants controls various growth and development aspects; however, the actual roles of these genes in flowering plants are not well known. In this study, a novel bHLH protein CmbHLH110 was found to interact with CmERF110 by and experiments, a chrysanthemum ERF110 homolog that acts as a positive flowering regulator. In addition, was also found to regulate the flowering of chrysanthemums, overexpression of causes chrysanthemums to flower earlier, and suppressed leads to delayed flowering. Furthermore, the loss-of-function mutant of its homologue () had a noticeable late flowering phenotype, and completely complemented the late flowering phenotype of the mutant, whereas heterologous overexpression of in Col-0 caused early flowering. Transcriptome sequencing revealed significant differential expression of flowering-related and circadian clock-related genes in transgenic chrysanthemum. Therefore, we concluded that CmbHLH110, as a novel flowering regulator, could interact with CmERF110 to regulate flowering in chrysanthemum.
期刊介绍:
Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.