J. V. Bochkova, W. Liu, N. A. Brazhe, A. A. Zhgun, G. V. Maksimov
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A Raman Spectroscopic Study of the Conformation of Flavin Adenine Dinucleotide, a CoEnzyme of D-Amino Acid Oxidase
The spectra of surface-enhanced Raman scattering of D-amino acid oxidase from pig kidney were recorded and analyzed using silver nanoparticles; characteristic spectral parameters of changes in the conformation of the flavinadenine dinucleotide cofactor during activation of the enzyme by D-amino acids were revealed. It was found that the time during which changes in the conformation of the flavinadenine dinucleotide were recorded depended on the substrate specificity of the enzyme: in the presence of D-alanine, this took several seconds, and in the presence of D-serine, minutes.
BiophysicsBiochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍：
Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
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