{"title":"芽孢杆菌在番茄根结线虫病防治中的应用效果","authors":"Yunhong Geng","doi":"10.1166/jbmb.2024.2365","DOIUrl":null,"url":null,"abstract":"Root-knot nematode is a kind of omnivorous plant pathogenic nematode that causes great harm to underground roots and above-ground parts of plants, and has become a serious factor restricting the development of global agriculture. In order to realize the sustainable development of agriculture,\n the research carried out an in-depth discussion on biological control methods. Firstly, a bacillus was obtained through insecticidal experiments, then a large amount of materials was obtained through purification and cultivation, and then the stability test of the active substance of Bacillus\n was conducted to explore its influence to root-knot nematodes. Finally, the pot verification experiment and field verification experiment were conducted. The results showed that in the insecticidal experiment, the accumulation of active substances at 14 h was the maximum, and the corresponding\n mortality of root-knot nematodes at 12 h and 24 h was 97.6% and 82.91%, respectively. In the BS pot experiment, the number of tomato root knot formation in the bacillus treated group was significantly reduced by 46.3% compared with the control group, and the number of egg sac of the rhizome\n was reduced by 78.31%. On the 120th day of field verification experiment, the fruit quantity and yield per plant in the treatment group increased by 17.02% and 11.85%, respectively. In summary, Bacillus can effectively reduce the damage of root knot nematode to tomato plants, and has a good\n prospect of application and popularization.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Application Effect of Bacillus in Tomato Root Knot Nematode Disease Control\",\"authors\":\"Yunhong Geng\",\"doi\":\"10.1166/jbmb.2024.2365\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Root-knot nematode is a kind of omnivorous plant pathogenic nematode that causes great harm to underground roots and above-ground parts of plants, and has become a serious factor restricting the development of global agriculture. In order to realize the sustainable development of agriculture,\\n the research carried out an in-depth discussion on biological control methods. Firstly, a bacillus was obtained through insecticidal experiments, then a large amount of materials was obtained through purification and cultivation, and then the stability test of the active substance of Bacillus\\n was conducted to explore its influence to root-knot nematodes. Finally, the pot verification experiment and field verification experiment were conducted. The results showed that in the insecticidal experiment, the accumulation of active substances at 14 h was the maximum, and the corresponding\\n mortality of root-knot nematodes at 12 h and 24 h was 97.6% and 82.91%, respectively. In the BS pot experiment, the number of tomato root knot formation in the bacillus treated group was significantly reduced by 46.3% compared with the control group, and the number of egg sac of the rhizome\\n was reduced by 78.31%. On the 120th day of field verification experiment, the fruit quantity and yield per plant in the treatment group increased by 17.02% and 11.85%, respectively. In summary, Bacillus can effectively reduce the damage of root knot nematode to tomato plants, and has a good\\n prospect of application and popularization.\",\"PeriodicalId\":15157,\"journal\":{\"name\":\"Journal of Biobased Materials and Bioenergy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biobased Materials and Bioenergy\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1166/jbmb.2024.2365\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biobased Materials and Bioenergy","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1166/jbmb.2024.2365","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
根结线虫是一种杂食性植物病原线虫,对植物地下根系和地上部分危害极大,已成为制约全球农业发展的严重因素。为了实现农业的可持续发展,该研究对生物防治方法进行了深入探讨。首先,通过杀虫实验获得了芽孢杆菌,然后通过纯化和培养获得了大量材料,接着进行了芽孢杆菌活性物质的稳定性试验,探讨其对根结线虫的影响。最后,进行了盆栽验证实验和田间验证实验。结果表明,在杀虫实验中,活性物质在 14 h 的积累量最大,相应的根结线虫在 12 h 和 24 h 的死亡率分别为 97.6%和 82.91%。在 BS 盆栽实验中,杆菌处理组的番茄根结形成数比对照组显著减少了 46.3%,根茎卵囊数减少了 78.31%。在田间试验的第 120 天,处理组的果实数量和单株产量分别增加了 17.02% 和 11.85%。综上所述,芽孢杆菌能有效减轻根结线虫对番茄植株的危害,具有良好的应用和推广前景。
Application Effect of Bacillus in Tomato Root Knot Nematode Disease Control
Root-knot nematode is a kind of omnivorous plant pathogenic nematode that causes great harm to underground roots and above-ground parts of plants, and has become a serious factor restricting the development of global agriculture. In order to realize the sustainable development of agriculture,
the research carried out an in-depth discussion on biological control methods. Firstly, a bacillus was obtained through insecticidal experiments, then a large amount of materials was obtained through purification and cultivation, and then the stability test of the active substance of Bacillus
was conducted to explore its influence to root-knot nematodes. Finally, the pot verification experiment and field verification experiment were conducted. The results showed that in the insecticidal experiment, the accumulation of active substances at 14 h was the maximum, and the corresponding
mortality of root-knot nematodes at 12 h and 24 h was 97.6% and 82.91%, respectively. In the BS pot experiment, the number of tomato root knot formation in the bacillus treated group was significantly reduced by 46.3% compared with the control group, and the number of egg sac of the rhizome
was reduced by 78.31%. On the 120th day of field verification experiment, the fruit quantity and yield per plant in the treatment group increased by 17.02% and 11.85%, respectively. In summary, Bacillus can effectively reduce the damage of root knot nematode to tomato plants, and has a good
prospect of application and popularization.