microRNA-320 纳米胶囊通过刺激骨髓间充质干细胞促进骨质疏松性骨折的治疗

IF 2.9 4区 医学 Q1 Medicine Journal of biomedical nanotechnology Pub Date : 2024-03-01 DOI:10.1166/jbn.2024.3784
Ligang Qian, Qinggui Li, Qiao Ren
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引用次数: 0

摘要

我们旨在探索microRNA-320(miR-320)在骨质疏松性骨折中的作用机制。我们制备了miR-320纳米颗粒,并用Zetasizer Nano和三乙胺(TEA)检测了其特性。然后,我们用 MTT 评估骨髓间充质干细胞的细胞毒性,并测定基因表达。我们建立了小鼠骨折模型,并用 miR-320 纳米粒子或孔隙纳米粒子进行处理。检测了 miR-320 的释放和骨折部位的骨修复情况。对基质金属蛋白酶(MMP)敏感的陶瓷基质复合材料(CMCS)(miR-320)在骨缺损处释放了 miR-320,促进了成骨基因的转录,刺激了骨的生成。最后,miR-320纳米颗粒的处理促进了小鼠骨质疏松缺损的骨修复。装载了miR-320的MMP敏感纳米胶囊能促进成骨潜能并刺激骨折修复,为骨质疏松性骨折的新型治疗方法提供了启示。
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Treatment Promotion of Osteoporotic Fractures by microRNA-320 Nanocapsules Through Stimulating Bone Marrow Mesenchymal Stem Cells
We aimed to explore the mechanism underlying microRNA-320 (miR-320)’s role in osteoporotic fractures. miR-320 nanoparticles were prepared and their characterization was detected by Zetasizer Nano and triethylamine (TEA). miR-320 nanoparticles were interacted with bone marrow mesenchymal stem cells (BMSCs). Then we conducted MTT to assess cytotoxicity in BMSCs and determined genes expression. A mouse fracture model was established and treated with miR-320 nanoparticles or pore nanoparticles. The release of miR-320 and the bone repair at the fracture site were detected. Treatment of Ceramic matrix composites (CMCS) (miR-320) sensitive to Matrix metalloproteinase (MMP) released miR-320 to bone defect, which promoted the transcription of osteogenic genes and stimulated the osteogenesis. Finally, treatment of miR-320 nanoparticles facilitated bone repair of mouse osteoporotic defect. MMP-sensitive nanocapsules loaded with miR-320 can promote osteogenic potential and stimulate fracture repair, providing insight into novel treatment against osteoporotic fracture.
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来源期刊
CiteScore
4.30
自引率
17.20%
发文量
145
审稿时长
2.3 months
期刊介绍: Information not localized
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