Y. F. Krupyanskii, N.G. Loiko, V. V. Kovalenko, A. A. Generalova, E. V. Tereshkin, A. V. Moiseenko, K. B. Tereshkina, A. N. Popov, O. S. Sokolova
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引用次数: 0
摘要
摘要 通过同步辐射衍射和透射电子显微镜研究了休眠细胞中 DNA 的结构。衍射实验表明，DNA出现了周期性的有序组织。透射电子显微镜可以直观地观察到DNA凝结类型的信息。我们发现了细胞内 DNA 的纳米晶、液晶和折叠核糖体状结构。接下来，我们研究了在暴露于无丝分裂自体诱导剂化学类似物（4-己基间苯二酚）的压力下 DNA 结构的变化。对缺氧状态和木乃伊化状态下的DNA结构的研究表明，缺氧状态下的DNA结构与饥饿胁迫下休眠状态下的DNA结构相同。木乃伊化状态下的 DNA 结构与无生物活性状态下的 DNA 结构截然不同。
Condensed DNA Structure in Bacteria Subjected to Various Types of Stress
Abstract
The architecture of DNA in the dormant cells was studied by synchrotron radiation diffraction and transmission electron microscopy. Diffraction experiments indicate the appearance of a periodic ordered organization of DNA. Transmission electron microscopy made it possible to visualize information about the type of DNA condensation. Intracellular nanocrystalline, liquid-crystalline and folded nucleosome-like structures of DNA have been found. Next, we studied changes in DNA architecture under stress of exposure to a chemical analogue of the anabiosis autoinducer (4-hexylresorcinol). Studies of the DNA architecture in the anabiotic and mummified states show the identity of the DNA structure in the anabiotic state and in the dormant state under starvation stress. The architecture of DNA in a mummified state is very different from that of DNA in an anabiotic state.
期刊介绍：
Moscow University Biological Sciences Bulletin is forum for research in all important areas of modern biology. It publishes original work on qualitative, analytical and experimental aspects of research. The scope of articles to be considered includes plant biology, zoology, ecology, evolutionary biology, biophysics, genetics, genomics, proteomics, molecular biology, cell biology, biochemistry, endocrinology, immunology, physiology, pharmacology, neuroscience, gerontology, developmental biology, bioinformatics, bioengineering, virology, and microbiology.
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