Heike Ef Becker, Nader Kameli, Alice Rustichelli, Britt Am Heijnens, Frank Rm Stassen, John Penders, Daisy Mae Jonkers
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引用次数: 0
摘要
目的：本研究旨在探讨活动期和缓解期克罗恩病（CD）患者的粪便水（FW）对粘蛋白降解和上皮屏障功能的影响。研究方法从 6 名健康对照组（HCs）和 12 名克罗恩病患者的新鲜粪便样本中分离出粪便水和细菌膜囊（MVs）。通过 16S rRNA 基因扩增片段测序确定细菌组成。结果体外 FW 诱导的粘蛋白降解率在 CD 样本中高于 HC 样本（p 结论：体外 FW 诱导的粘蛋白降解率在 CD 样本中高于 HC 样本：CD 来源的 FW 具有更高的粘蛋白降解能力，这可能表明微生物产物对 CD 的病理生理学有贡献。
In vitro mucin degradation and paracellular permeability by fecal water from Crohn's disease patients.
Aim: This study aimed to examine the impact of fecal water (FW) of active and remissive Crohn's disease (CD) patients on mucin degradation and epithelial barrier function. Methods: FW and bacterial membrane vesicles (MVs) were isolated from fresh fecal samples of six healthy controls (HCs) and 12 CD patients. Bacterial composition was determined by 16S rRNA gene amplicon sequencing. Results:In vitro FW-induced mucin degradation was higher in CD samples versus HC (p < 0.01), but not associated with specific bacterial genera. FW of three remissive samples decreased transepithelial electrical resistance in Caco-2 cells by 78-87% (p < 0.001). MVs did not induce barrier alterations. Conclusion: The higher mucin-degradation capacity of CD-derived FW might suggest contributions of microbial products to CD pathophysiology.
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