胃癌患者胃液的挥发性特征

IF 3.7 4区 医学 Q1 BIOCHEMICAL RESEARCH METHODS Journal of breath research Pub Date : 2024-03-21 DOI:10.1088/1752-7163/ad324f
Linda Mezmale, Daria Ślefarska-Wolak, Manohar Prasad Bhandari, Clemens Ager, Viktors Veliks, Veronika Patsko, Andrii Lukashenko, Emmanuel Dias-Neto, Diana Noronha Nunes, Thais Fernanda Bartelli, Adriane Graicer Pelosof, Claudia Zitron Sztokfisz, Raúl Murillo, Agnieszka Królicka, Chris A Mayhew, Marcis Leja, Hossam Haick, Pawel Mochalski
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引用次数: 0

摘要

挥发物组学是一种强大的工具,能够为胃癌诊断提供新型生物标记物。本研究的主要目的是描述胃液的挥发性特征,以确定胃癌诱发的潜在变化。研究人员采用气相色谱-质谱检测(GC-MS)技术,结合顶空固相微萃取(HS-SPME)作为预浓缩技术,鉴定了从四个不同地点(拉脱维亚、乌克兰、巴西和哥伦比亚)收集的 78 名胃癌患者和两组对照组(分别为 80 名和 96 名受试者)的胃液样本中释放的挥发性有机化合物(VOC)。从患者提供的样本中鉴定出了 1440 种不同的化合物,从对照组提供的样本中鉴定出了 1422 种不同的化合物。然而,只有 6% 的挥发性有机化合物的发生率高于 20%。在散发的挥发性物质中,有 18 种物质在癌症患者和对照组的胃液中的顶空浓度存在差异。其中 10 种(1-丙醇、2,3-丁二酮、2-戊酮、苯乙醛、3-甲基丁醛、丁基羟基甲苯、2-戊基呋喃、2-乙基己醛、2-甲基丙醛和苯酚)在患者胃液样本顶空气中的含量明显较高;而 8 种在患者体内的含量低于对照组。鉴于挥发性特征的差异可以用某些酶或途径的活性发生与癌症相关的变化来解释,前一组可被视为胃癌的潜在生物标记物,这可能有助于开发用于诊断这种疾病的非侵入性呼气测试。要进一步阐明胃癌导致挥发物变化的机制,还需要进一步研究。 ClinicalTrials.gov ID NCT04022109 .
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Volatilomic profiles of gastric juice in gastric cancer patients.

Volatilomics is a powerful tool capable of providing novel biomarkers for the diagnosis of gastric cancer. The main objective of this study was to characterize the volatilomic signatures of gastric juice in order to identify potential alterations induced by gastric cancer. Gas chromatography with mass spectrometric detection, coupled with headspace solid phase microextraction as the pre-concentration technique, was used to identify volatile organic compounds (VOCs) released by gastric juice samples collected from 78 gastric cancer patients and two cohorts of controls (80 and 96 subjects) from four different locations (Latvia, Ukraine, Brazil, and Colombia). 1440 distinct compounds were identified in samples obtained from patients and 1422 in samples provided by controls. However, only 6% of the VOCs exhibited an incidence higher than 20%. Amongst the volatiles emitted, 18 showed differences in their headspace concentrations above gastric juice of cancer patients and controls. Ten of these (1-propanol, 2,3-butanedione, 2-pentanone, benzeneacetaldehyde, 3-methylbutanal, butylated hydroxytoluene, 2-pentyl-furan, 2-ethylhexanal, 2-methylpropanal and phenol) appeared at significantly higher levels in the headspace of the gastric juice samples obtained from patients; whereas, eight species showed lower abundance in patients than found in controls. Given that the difference in the volatilomic signatures can be explained by cancer-related changes in the activity of certain enzymes or pathways, the former set can be considered potential biomarkers for gastric cancer, which may assist in developing non-invasive breath tests for the diagnosis of this disease. Further studies are required to elucidate further the mechanisms that underlie the changes in the volatilomic profile as a result of gastric cancer.

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来源期刊
Journal of breath research
Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM
CiteScore
7.60
自引率
21.10%
发文量
49
审稿时长
>12 weeks
期刊介绍: Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.
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