Haya Doura Alomari, Yasser Alsayed Tolibah, Chaza Kouchaji
{"title":"BioMin F 和 NovaMin 牙膏对变异链球菌的功效:一项体外研究。","authors":"Haya Doura Alomari, Yasser Alsayed Tolibah, Chaza Kouchaji","doi":"10.1038/s41405-024-00202-x","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This in vitro study was accomplished to demonstrate the antibacterial efficacy of BioMin F and NovaMin toothpastes against the recently-isolated Streptococcus Mutans in comparison with a commonly used fluoride toothpaste.</p><p><strong>Materials and methods: </strong>Dental plaque collection method was adopted to isolate streptococcus mutans in children with dental caries. Then an ideal Streptococcus Mutans colony was incubated in 20 Petri dishes that contained Mueller-Hinton medium. Each dish had 3 wells; one well for each toothpaste (BioMin F, NovMin, and Signal) to perform the agar diffusion test. After incubating for 24 hours, the inhabitation zone around each well of each Petri dish was noticed and measured. Statistical Analysis was achieved using a statistical package, SPSS Windows version 17, by applying Kruskal-Wallis with Mann-Whitney U test (α = 0.05).</p><p><strong>Results: </strong>BioMin F showed the highest mean of inhibition zone diameter ( <math> <mover><mrow><mi>x</mi></mrow> <mo>¯</mo></mover> </math> = 2.67 mm) in compared with NovaMin and Signal ( <math> <mover><mrow><mi>x</mi></mrow> <mo>¯</mo></mover> </math> = 0.39 mm and <math> <mover><mrow><mi>x</mi></mrow> <mo>¯</mo></mover> </math> = 2.19 mm; p < 0.001 in each pairwise comparison).</p><p><strong>Conclusion: </strong>BioMin F toothpaste showed superior antibacterial effect against Streptococcus mutans to Signal and NovaMin toothpastes. Novamin showed the lowest antibacterial effect. This in vitro study suggests that BioMin F toothpaste shows encouraging potential to be recommended as a preventive measure to reduce the caries risk.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10928140/pdf/","citationCount":"0","resultStr":"{\"title\":\"Efficacy of BioMin F and NovaMin toothpastes against streptococcus mutans: an in vitro study.\",\"authors\":\"Haya Doura Alomari, Yasser Alsayed Tolibah, Chaza Kouchaji\",\"doi\":\"10.1038/s41405-024-00202-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This in vitro study was accomplished to demonstrate the antibacterial efficacy of BioMin F and NovaMin toothpastes against the recently-isolated Streptococcus Mutans in comparison with a commonly used fluoride toothpaste.</p><p><strong>Materials and methods: </strong>Dental plaque collection method was adopted to isolate streptococcus mutans in children with dental caries. Then an ideal Streptococcus Mutans colony was incubated in 20 Petri dishes that contained Mueller-Hinton medium. Each dish had 3 wells; one well for each toothpaste (BioMin F, NovMin, and Signal) to perform the agar diffusion test. After incubating for 24 hours, the inhabitation zone around each well of each Petri dish was noticed and measured. Statistical Analysis was achieved using a statistical package, SPSS Windows version 17, by applying Kruskal-Wallis with Mann-Whitney U test (α = 0.05).</p><p><strong>Results: </strong>BioMin F showed the highest mean of inhibition zone diameter ( <math> <mover><mrow><mi>x</mi></mrow> <mo>¯</mo></mover> </math> = 2.67 mm) in compared with NovaMin and Signal ( <math> <mover><mrow><mi>x</mi></mrow> <mo>¯</mo></mover> </math> = 0.39 mm and <math> <mover><mrow><mi>x</mi></mrow> <mo>¯</mo></mover> </math> = 2.19 mm; p < 0.001 in each pairwise comparison).</p><p><strong>Conclusion: </strong>BioMin F toothpaste showed superior antibacterial effect against Streptococcus mutans to Signal and NovaMin toothpastes. Novamin showed the lowest antibacterial effect. This in vitro study suggests that BioMin F toothpaste shows encouraging potential to be recommended as a preventive measure to reduce the caries risk.</p>\",\"PeriodicalId\":36997,\"journal\":{\"name\":\"BDJ Open\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-03-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10928140/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BDJ Open\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1038/s41405-024-00202-x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BDJ Open","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1038/s41405-024-00202-x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
摘要
研究目的本体外研究旨在证明 BioMin F 和 NovaMin 牙膏与常用含氟牙膏相比,对最近分离出的变异链球菌的抗菌效果:采用牙菌斑采集法分离龋齿儿童中的变异链球菌。然后将理想的变异链球菌菌落培养在装有穆勒-欣顿培养基的 20 个培养皿中。每个培养皿有 3 个孔;每种牙膏(BioMin F、NovMin 和 Signal)一个孔,以进行琼脂扩散试验。培养 24 小时后,观察并测量每个培养皿中每个孔周围的栖息区。使用 SPSS Windows 17 版统计软件包进行统计分析,采用 Kruskal-Wallis 和 Mann-Whitney U 检验(α = 0.05):与 NovaMin 和 Signal(x¯ = 0.39 mm 和 x¯ = 2.19 mm;p)相比,BioMin F 的抑菌区直径(x¯ = 2.67 mm)平均值最高:BioMin F 牙膏对变异链球菌的抗菌效果优于 Signal 和 NovaMin 牙膏。Novamin 的抗菌效果最低。这项体外研究表明,BioMin F 牙膏具有令人鼓舞的潜力,可推荐用作降低龋齿风险的预防措施。
Efficacy of BioMin F and NovaMin toothpastes against streptococcus mutans: an in vitro study.
Objective: This in vitro study was accomplished to demonstrate the antibacterial efficacy of BioMin F and NovaMin toothpastes against the recently-isolated Streptococcus Mutans in comparison with a commonly used fluoride toothpaste.
Materials and methods: Dental plaque collection method was adopted to isolate streptococcus mutans in children with dental caries. Then an ideal Streptococcus Mutans colony was incubated in 20 Petri dishes that contained Mueller-Hinton medium. Each dish had 3 wells; one well for each toothpaste (BioMin F, NovMin, and Signal) to perform the agar diffusion test. After incubating for 24 hours, the inhabitation zone around each well of each Petri dish was noticed and measured. Statistical Analysis was achieved using a statistical package, SPSS Windows version 17, by applying Kruskal-Wallis with Mann-Whitney U test (α = 0.05).
Results: BioMin F showed the highest mean of inhibition zone diameter ( = 2.67 mm) in compared with NovaMin and Signal ( = 0.39 mm and = 2.19 mm; p < 0.001 in each pairwise comparison).
Conclusion: BioMin F toothpaste showed superior antibacterial effect against Streptococcus mutans to Signal and NovaMin toothpastes. Novamin showed the lowest antibacterial effect. This in vitro study suggests that BioMin F toothpaste shows encouraging potential to be recommended as a preventive measure to reduce the caries risk.