测量和控制哺乳动物细胞培养过程中泡沫的产生。

IF 2.5 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology Progress Pub Date : 2024-03-12 DOI:10.1002/btpr.3450
James Flynn, Laura Breen, Shankara Narayanan, Michael Butler
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引用次数: 0

摘要

过度搅拌或气体喷射会在哺乳动物细胞培养物中产生泡沫。尤其是在培养产生高浓度重组蛋白的细胞时,更容易产生泡沫。我们测试了三种消泡剂与产生抗体的中国仓鼠卵巢(CHO)细胞的相容性。一种药剂(消泡剂 204)在 10 ppm 的浓度下完全抑制细胞生长,一种药剂(消泡剂 C)表现出部分抑制作用,第三种药剂(消泡剂 SE-15)在此浓度下没有抑制作用。使用新型泡沫图像分析仪(LabCam)对两种消泡剂(C 和 SE-15)进行了评估,以确定它们通过加强搅拌消散细胞培养基中产生的泡沫的能力。培养基中消泡剂的存在大大减少了产生的泡沫层,而且在 10 ppm SE-15 的存在下,泡沫层会迅速消散。此外,还测试了消泡剂在大于 106 个细胞/毫升的 CHO 细胞培养物中的消泡效果。在培养物中添加 SE-15 后,过量气体喷射产生的泡沫在 2 分钟内消散。在同等条件下,有消泡剂 C 存在时,75% 的泡沫在 2 分钟内消散,但残留泡沫层长达 25 分钟。这项研究显示了光学监测系统(LabCam)在测量生物反应器中泡沫的产生和消散方面的价值,可用于评估消泡剂在生物反应器中减少泡沫的效率。该系统可用作控制系统,用于哺乳动物细胞生物处理过程中的连续监测和泡沫控制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Measurement and control of foam generation in a mammalian cell culture

Foam is generated in mammalian cell cultures by excessive agitation or gas sparging. This occurs particularly in cultures that generate recombinant proteins at high cell concentrations. Three antifoam agents were tested for their compatibility with antibody-producing Chinese hamster ovary (CHO) cells. One agent (antifoam 204) was completely inhibitory to growth at a concentration of 10 ppm, one agent (antifoam C) showed partial inhibition and a third (antifoam SE-15) showed no inhibition at this concentration. A novel foam image analyzer (LabCam) was used to evaluate two antifoams (C and SE-15) for their ability to dissipate foam generated in cell culture media by enhanced agitation. The presence of antifoam in the media reduced significantly the foam layer that was generated and this was shown to be rapidly dissipated in the presence of 10 ppm SE-15. The antifoams were also tested for foam dissipation in cultures of CHO cells at >106 cells/mL. Supplementation of the cultures with SE-15 resulted in dissipation of foam generated by excessive gas sparging within 2 min. Under equivalent conditions 75% of foam dissipated in the presence of antifoam C, within 2 min but there was a residual foam layer up to 25 min. This study showed the value of an optical monitoring system (LabCam) for measuring foam generation and dissipation in a bioreactor to assess the efficiency of antifoam agents to reduce foam in a bioreactor. This has the potential for use as a control system that could be designed for continuous monitoring and foam control in a mammalian cell bioprocess.

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来源期刊
Biotechnology Progress
Biotechnology Progress 工程技术-生物工程与应用微生物
CiteScore
6.50
自引率
3.40%
发文量
83
审稿时长
4 months
期刊介绍: Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.
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