M. Bianchi , E.V. Paravani , M.G. Acosta , L.M. Odetti , M.F. Simoniello , G.L. Poletta
{"title":"农药诱导斑马鱼(Danio rerio)行为、组织学、DNA 损伤和 mRNA 表达的改变:综合方法。","authors":"M. Bianchi , E.V. Paravani , M.G. Acosta , L.M. Odetti , M.F. Simoniello , G.L. Poletta","doi":"10.1016/j.cbpc.2024.109895","DOIUrl":null,"url":null,"abstract":"<div><p>To assess the impact of glyphosate and 2,4-D herbicides, as well as the insecticide imidacloprid, both individually and in combination, the gills of adult zebrafish were used due to their intimate interaction with chemicals diluted in water. Bioassays were performed exposing the animals to the different pesticides and their mixture for 96 h. The behavior of the fish was analyzed, a histological examination of the gills was carried out, and the genotoxic effects were also analyzed by means of the comet assay (CA) and the change in the expression profiles of genes involved in the pathways of the oxidative stress and cellular apoptosis. The length traveled and the average speed of the control fish, compared to those exposed to the pesticides and mainly those exposed to the mixture, were significantly greater. All the groups exposed individually exhibited a decrease in thigmotaxis time, indicating a reduction in the behavior of protecting themselves from predators. Histological analysis revealed significant differences in the structures of the gill tissues. The quantification of the histological lesions showed mild lesions in the fish exposed to imidacloprid, moderate to severe lesions for glyphosate, and severe lesions in the case of 2,4-D and the mixture of pesticides. The CA revealed the sensitivity of gill cells to DNA damage following exposure to glyphosate, 2,4-D, imidacloprid and the mixture. 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引用次数: 0
摘要
为了评估草甘膦、2,4-D 除草剂以及杀虫剂吡虫啉单独或混合使用时的影响,研究人员使用了成年斑马鱼的鳃,因为它们与稀释在水中的化学品有密切的相互作用。生物测定将斑马鱼与不同的杀虫剂及其混合物接触 96 小时。分析了鱼的行为,对鱼鳃进行了组织学检查,还通过彗星试验(CA)分析了基因毒性效应,以及氧化应激和细胞凋亡途径相关基因表达谱的变化。与接触杀虫剂的鱼类(主要是接触混合物的鱼类)相比,对照组鱼类的游动长度和平均速度明显增加。所有单独暴露的组别都表现出游曳时间的减少,这表明鱼类保护自己免受捕食者伤害的行为减少了。组织学分析表明,鳃组织结构存在明显差异。对组织学病变的定量分析显示,接触吡虫啉的鱼类出现轻微病变,草甘膦出现中度到重度病变,而 2,4-D 和混合农药则出现重度病变。CA显示了鱼鳃细胞在接触草甘膦、2,4-D、吡虫啉和混合物后对DNA损伤的敏感性。最后,参与氧化应激途径的基因和与细胞凋亡途径有关的基因都出现了过表达,而参与 DNA 修复的 ogg1 基因则出现了下调。
Pesticide-induced alterations in zebrafish (Danio rerio) behavior, histology, DNA damage and mRNA expression: An integrated approach
To assess the impact of glyphosate and 2,4-D herbicides, as well as the insecticide imidacloprid, both individually and in combination, the gills of adult zebrafish were used due to their intimate interaction with chemicals diluted in water. Bioassays were performed exposing the animals to the different pesticides and their mixture for 96 h. The behavior of the fish was analyzed, a histological examination of the gills was carried out, and the genotoxic effects were also analyzed by means of the comet assay (CA) and the change in the expression profiles of genes involved in the pathways of the oxidative stress and cellular apoptosis. The length traveled and the average speed of the control fish, compared to those exposed to the pesticides and mainly those exposed to the mixture, were significantly greater. All the groups exposed individually exhibited a decrease in thigmotaxis time, indicating a reduction in the behavior of protecting themselves from predators. Histological analysis revealed significant differences in the structures of the gill tissues. The quantification of the histological lesions showed mild lesions in the fish exposed to imidacloprid, moderate to severe lesions for glyphosate, and severe lesions in the case of 2,4-D and the mixture of pesticides. The CA revealed the sensitivity of gill cells to DNA damage following exposure to glyphosate, 2,4-D, imidacloprid and the mixture. Finally, both genes involved in the oxidative stress pathway and those related to the cell apoptosis pathway were overexpressed, while the ogg1 gene, involved in DNA repair, was downregulated.
期刊介绍:
Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.