{"title":"使用抗氧化剂延长冻干无细胞合成系统的储存时间","authors":"Kyu Jae Kim, So Jeong Lee, Dong-Myung Kim","doi":"10.1007/s12257-024-00054-0","DOIUrl":null,"url":null,"abstract":"<p>Modern chemical processes, vital for diverse product manufacturing, often result in substantial energy consumption and environmental waste. As environmental concerns continue to escalate and industries evolve to meet customized demands, biomanufacturing emerges as a promising alternative due to its efficiency, expandability, and eco-friendliness. Cell-free synthesis systems, which harness cellular extracts for biosynthetic reactions, offer a highly adaptable solution for biomanufacturing, particularly when rapid production is required with limited resources. While conventional cell-free systems encounter challenges related to storage and transportation due to the necessity for ultra-cold temperatures, recent studies have demonstrated that these systems can be lyophilized and rehydrated to enable on-demand biomolecule production. Our study aims to enhance the stability of lyophilized cell-free systems. We have discovered the significant role played by antioxidants, specifically dithiothreitol and sodium nitrite, in preserving translational activity during extended storage. This finding represents a significant step forward for decentralized, on-demand protein production using cell-free methods.</p>","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Use of antioxidants to extend the storage of lyophilized cell-free synthesis system\",\"authors\":\"Kyu Jae Kim, So Jeong Lee, Dong-Myung Kim\",\"doi\":\"10.1007/s12257-024-00054-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Modern chemical processes, vital for diverse product manufacturing, often result in substantial energy consumption and environmental waste. As environmental concerns continue to escalate and industries evolve to meet customized demands, biomanufacturing emerges as a promising alternative due to its efficiency, expandability, and eco-friendliness. Cell-free synthesis systems, which harness cellular extracts for biosynthetic reactions, offer a highly adaptable solution for biomanufacturing, particularly when rapid production is required with limited resources. While conventional cell-free systems encounter challenges related to storage and transportation due to the necessity for ultra-cold temperatures, recent studies have demonstrated that these systems can be lyophilized and rehydrated to enable on-demand biomolecule production. Our study aims to enhance the stability of lyophilized cell-free systems. We have discovered the significant role played by antioxidants, specifically dithiothreitol and sodium nitrite, in preserving translational activity during extended storage. This finding represents a significant step forward for decentralized, on-demand protein production using cell-free methods.</p>\",\"PeriodicalId\":8936,\"journal\":{\"name\":\"Biotechnology and Bioprocess Engineering\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-03-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotechnology and Bioprocess Engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s12257-024-00054-0\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology and Bioprocess Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s12257-024-00054-0","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Use of antioxidants to extend the storage of lyophilized cell-free synthesis system
Modern chemical processes, vital for diverse product manufacturing, often result in substantial energy consumption and environmental waste. As environmental concerns continue to escalate and industries evolve to meet customized demands, biomanufacturing emerges as a promising alternative due to its efficiency, expandability, and eco-friendliness. Cell-free synthesis systems, which harness cellular extracts for biosynthetic reactions, offer a highly adaptable solution for biomanufacturing, particularly when rapid production is required with limited resources. While conventional cell-free systems encounter challenges related to storage and transportation due to the necessity for ultra-cold temperatures, recent studies have demonstrated that these systems can be lyophilized and rehydrated to enable on-demand biomolecule production. Our study aims to enhance the stability of lyophilized cell-free systems. We have discovered the significant role played by antioxidants, specifically dithiothreitol and sodium nitrite, in preserving translational activity during extended storage. This finding represents a significant step forward for decentralized, on-demand protein production using cell-free methods.
期刊介绍:
Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.