使用抗氧化剂延长冻干无细胞合成系统的储存时间

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioprocess Engineering Pub Date : 2024-03-13 DOI:10.1007/s12257-024-00054-0
Kyu Jae Kim, So Jeong Lee, Dong-Myung Kim
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引用次数: 0

摘要

现代化学工艺对各种产品的生产至关重要,但往往造成大量能源消耗和环境浪费。随着对环境问题的关注不断升级,以及工业不断发展以满足定制化需求,生物制造因其高效性、可扩展性和生态友好性而成为一种前景广阔的替代方案。无细胞合成系统利用细胞提取物进行生物合成反应,为生物制造提供了一种适应性很强的解决方案,尤其是在需要利用有限资源进行快速生产的情况下。传统的无细胞系统由于必须在超低温条件下进行,因此在储存和运输方面遇到了挑战,而最近的研究表明,这些系统可以冻干和再水化,从而实现按需生产生物大分子。我们的研究旨在提高冻干无细胞系统的稳定性。我们发现了抗氧化剂(特别是二硫苏糖醇和亚硝酸钠)在长时间储存过程中保持翻译活性的重要作用。这一发现标志着利用无细胞方法按需分散生产蛋白质向前迈出了重要一步。
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Use of antioxidants to extend the storage of lyophilized cell-free synthesis system

Modern chemical processes, vital for diverse product manufacturing, often result in substantial energy consumption and environmental waste. As environmental concerns continue to escalate and industries evolve to meet customized demands, biomanufacturing emerges as a promising alternative due to its efficiency, expandability, and eco-friendliness. Cell-free synthesis systems, which harness cellular extracts for biosynthetic reactions, offer a highly adaptable solution for biomanufacturing, particularly when rapid production is required with limited resources. While conventional cell-free systems encounter challenges related to storage and transportation due to the necessity for ultra-cold temperatures, recent studies have demonstrated that these systems can be lyophilized and rehydrated to enable on-demand biomolecule production. Our study aims to enhance the stability of lyophilized cell-free systems. We have discovered the significant role played by antioxidants, specifically dithiothreitol and sodium nitrite, in preserving translational activity during extended storage. This finding represents a significant step forward for decentralized, on-demand protein production using cell-free methods.

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来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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