COVID-19 患者的血浆不会改变体外人类内皮血脑屏障的抗电性

IF 5.1 Q2 CELL BIOLOGY Function (Oxford, England) Pub Date : 2024-01-09 eCollection Date: 2024-01-01 DOI:10.1093/function/zqae002
Agnė Pociūtė, Karolina Kriaučiūnaitė, Aida Kaušylė, Birutė Zablockienė, Tadas Alčauskas, Augustė Jelinskaitė, Akvilė Rudėnaitė, Ligita Jančorienė, Saulius Ročka, Alexei Verkhratsky, Augustas Pivoriūnas
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引用次数: 0

摘要

由严重急性呼吸系统综合征冠状病毒 2 引起的 2019 年冠状病毒病(COVID-19)大流行引发了最严重的全球健康危机。COVID-19 的临床表现通常包括严重的神经和神经精神症状。然而,目前尚不清楚血脑屏障(BBB)的病理损伤是否以及在多大程度上导致了 COVID-19 进展过程中神经病理学的发展。在本研究中,我们使用诱导多能干细胞衍生的脑内皮细胞(iBECs)研究了 COVID-19 患者血浆对体外 BBB 完整性的影响。我们还对 COVID-19 患者、健康人和康复者血浆中的细胞因子和趋化因子谱进行了全面分析。我们发现 COVID-19 患者血浆中干扰素 γ 诱导的 10 kDa 蛋白、肝细胞生长因子和白细胞介素-18 的水平明显升高。然而,COVID-19 患者的血浆并不影响 iBEC 单层的跨内皮电阻。我们的研究结果表明,COVID-19 患者血浆中的炎症因子不会直接影响 BBB 旁通路,这表明 COVID-19 期间 BBB 的病理重塑(如果有的话)可能是通过间接或未知的机制发生的。
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Plasma of COVID-19 Patients Does Not Alter Electrical Resistance of Human Endothelial Blood-Brain Barrier In Vitro.

The pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 instigated the most serious global health crisis. Clinical presentation of COVID-19 frequently includes severe neurological and neuropsychiatric symptoms. However, it is presently unknown whether and to which extent pathological impairment of blood-brain barrier (BBB) contributes to the development of neuropathology during COVID-19 progression. In the present study, we used human induced pluripotent stem cells-derived brain endothelial cells (iBECs) to study the effects of blood plasma derived from COVID-19 patients on the BBB integrity in vitro. We also performed a comprehensive analysis of the cytokine and chemokine profiles in the plasma of COVID-19 patients, healthy and recovered individuals. We found significantly increased levels of interferon γ-induced protein 10 kDa, hepatocyte growth factor, and interleukin-18 in the plasma of COVID-19 patients. However, blood plasma from COVID-19 patients did not affect transendothelial electrical resistance in iBEC monolayers. Our results demonstrate that COVID-19-associated blood plasma inflammatory factors do not affect BBB paracellular pathway directly and suggest that pathological remodeling (if any) of BBB during COVID-19 may occur through indirect or yet unknown mechanisms.

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