基于 CRISPR/Cas12a 技术的 MicroRNA 传感器:从间接检测到直接检测的演变。

IF 4.2 2区 化学 Q1 CHEMISTRY, ANALYTICAL Critical reviews in analytical chemistry Pub Date : 2024-03-15 DOI:10.1080/10408347.2024.2329229
Songcheng Yu, Xueying Lei, Chenling Qu
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引用次数: 0

摘要

微小核糖核酸(miRNA)已成为一种有前途的疾病诊断生物标志物和药物开发的潜在治疗靶点。检测 miRNA 可作为疾病诊断和预测疾病预后的非侵入性工具。CRISPR/Cas12a 系统因其高灵敏度和特异性,在核酸检测方面具有巨大潜力,已被开发为基于核酸检测各领域靶标的多功能工具。然而,基于CRISPR/Cas12a系统的miRNA检测必须经过或不经过扩增操作将RNA转化为DNA,因为含有PAM序列的dsDNA或ssDNA传统上被认为是Cas12a的激活剂。直到最近,才有通过 CRISPR/Cas12a 系统直接检测 miRNA 的报道。在这篇综述中,我们概述了基于 CRISPR/Cas12a 检测 miRNA 的生物传感器从间接到直接的演变过程,这将有利于开发性能更好的基于 CRISPR/Cas12a 的直接检测 miRNA 的传感器。
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MicroRNA Sensors Based on CRISPR/Cas12a Technologies: Evolution From Indirect to Direct Detection.

MicroRNA (miRNA) has emerged as a promising biomarker for disease diagnosis and a potential therapeutic targets for drug development. The detection of miRNA can serve as a noninvasive tool in diseases diagnosis and predicting diseases prognosis. CRISPR/Cas12a system has great potential in nucleic acid detection due to its high sensitivity and specificity, which has been developed to be a versatile tool for nucleic acid-based detection of targets in various fields. However, conversion from RNA to DNA with or without amplification operation is necessary for miRNA detection based on CRISPR/Cas12a system, because dsDNA containing PAM sequence or ssDNA is traditionally considered as the activator of Cas12a. Until recently, direct detection of miRNA by CRISPR/Cas12a system has been reported. In this review, we provide an overview of the evolution of biosensors based on CRISPR/Cas12a for miRNA detection from indirect to direct, which would be beneficial to the development of CRISPR/Cas12a-based sensors with better performance for direct detection of miRNA.

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来源期刊
CiteScore
12.00
自引率
4.00%
发文量
137
审稿时长
6 months
期刊介绍: Critical Reviews in Analytical Chemistry continues to be a dependable resource for both the expert and the student by providing in-depth, scholarly, insightful reviews of important topics within the discipline of analytical chemistry and related measurement sciences. The journal exclusively publishes review articles that illuminate the underlying science, that evaluate the field''s status by putting recent developments into proper perspective and context, and that speculate on possible future developments. A limited number of articles are of a "tutorial" format written by experts for scientists seeking introduction or clarification in a new area. This journal serves as a forum for linking various underlying components in broad and interdisciplinary means, while maintaining balance between applied and fundamental research. Topics we are interested in receiving reviews on are the following: · chemical analysis; · instrumentation; · chemometrics; · analytical biochemistry; · medicinal analysis; · forensics; · environmental sciences; · applied physics; · and material science.
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