丙型肝炎病毒感染者的 IFN-γ、IL-17、IL-22+ CD4+ 亚群及其与临床因素的相关性。

IF 1.4 Q4 IMMUNOLOGY American journal of clinical and experimental immunology Pub Date : 2024-02-25 eCollection Date: 2024-01-01
Soolmaz Khansalar, Zahra Faghih, Shaghik Barani, Mehdi Kalani, Mohammad Reza Ataollahi, Zeinab Mohammadi, Sepideh Namdari, Kurosh Kalantar
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引用次数: 0

摘要

背景:HCV感染中的CD4+T细胞反应在丙型肝炎病毒(HCV)感染的免疫病理学中起着至关重要的作用。我们的目的是调查 HCV 感染者体内 Th1、Th17 和 Th22 细胞的频率,并阐明它们在疾病进展中的作用:方法:我们招募了 26 名 HCV 感染者和 26 名健康人。采用流式细胞术对外周血单核细胞(PBMCs)进行染色,以分离产生 CD4、IFN-γ、IL-17 和 IL-22 的细胞:结果显示:与健康对照组相比,HCV 感染者 CD4+ T 细胞中 IL-22 的平均表达量明显较低。关于与临床因素和 T 亚群的相关性,在患者中观察到 CD4+ IFN-γ+ 细胞的频率与甲状腺素水平(T4)呈负相关。数据显示,促甲状腺激素(TSH)、胆固醇水平与 Th17 细胞的频率呈正相关。此外,血清肌酐水平与 Th1 和 Th17 细胞之间也存在正相关。最后,研究发现病毒负荷与 IL-17+ IL-22+ 细胞之间存在正相关,而病毒负荷与纯 Th22 细胞之间存在负相关:我们的研究结果表明,Th22细胞可能在HCV的免疫病理中起一定作用,并显示了Thlper亚群与疾病临床症状之间的关联。
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IFN-γ, IL-17, IL-22+ CD4+ subset in patients with hepatitis C virus and correlation with clinical factor.

Background: CD4+ T cell responses in HCV infection have a crucial role in the immunopathology of hepatitis C virus (HCV) infection. Our aim was to investigate the frequency of Th1, Th17, and Th22 cells in HCV-infected patients and elucidate their role in the progression of the disease.

Methods: Twenty-six HCV-infected patients and 26 healthy individuals were recruited. Peripheral blood mononuclear cells (PBMCs) were stained to separate CD4, IFN-γ, IL-17, and IL-22 producing cells using flow cytometry.

Results: Results showed that the mean expression of IL-22 in CD4+ T cells was significantly lower in HCV-infected patients compared to healthy controls. About correlation with clinical factor and T subsets, a negative correlation between the frequency of CD4+ IFN-γ+ cells and Thyroxine level (T4) was observed in the patients. The data showed a positive link between thyroid-stimulating hormone (TSH), cholesterol levels, and the frequency of Th17 cells. In addition, a positive correlation was seen between serum creatinine level with both Th1 and Th17. Ultimately, it was found that there was a positive link between viral burden and IL-17+ IL-22+ cells and a negative correlation between viral load and pure Th22.

Conclusions: Our findings indicate that Th22 cells may play a part in the immunopathology of HCV and show the associations between Thelper subsets and the clinical signs of the disease.

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