{"title":"用于数据存储的高通量 DNA 合成","authors":"Meng Yu, Xiaohui Tang, Zhenhua Li, Weidong Wang, Shaopeng Wang, Min Li, Qiuliyang Yu, Sijia Xie, Xiaolei Zuo and Chang Chen","doi":"10.1039/D3CS00469D","DOIUrl":null,"url":null,"abstract":"<p >With the explosion of digital world, the dramatically increasing data volume is expected to reach 175 ZB (1 ZB = 10<small><sup>12</sup></small> GB) in 2025. Storing such huge global data would consume tons of resources. Fortunately, it has been found that the deoxyribonucleic acid (DNA) molecule is the most compact and durable information storage medium in the world so far. Its high coding density and long-term preservation properties make itself one of the best data storage carriers for the future. High-throughput DNA synthesis is a key technology for “DNA data storage”, which encodes binary data stream (0/1) into quaternary long DNA sequences consisting of four bases (A/G/C/T). In this review, the workflow of DNA data storage and the basic methods of artificial DNA synthesis technology are outlined first. Then, the technical characteristics of different synthesis methods and the state-of-the-art of representative commercial companies, with a primary focus on silicon chip microarray-based synthesis and novel enzymatic DNA synthesis are presented. Finally, the recent status of DNA storage and new opportunities for future development in the field of high-throughput, large-scale DNA synthesis technology are summarized.</p>","PeriodicalId":68,"journal":{"name":"Chemical Society Reviews","volume":null,"pages":null},"PeriodicalIF":40.4000,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/cs/d3cs00469d?page=search","citationCount":"0","resultStr":"{\"title\":\"High-throughput DNA synthesis for data storage\",\"authors\":\"Meng Yu, Xiaohui Tang, Zhenhua Li, Weidong Wang, Shaopeng Wang, Min Li, Qiuliyang Yu, Sijia Xie, Xiaolei Zuo and Chang Chen\",\"doi\":\"10.1039/D3CS00469D\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >With the explosion of digital world, the dramatically increasing data volume is expected to reach 175 ZB (1 ZB = 10<small><sup>12</sup></small> GB) in 2025. Storing such huge global data would consume tons of resources. Fortunately, it has been found that the deoxyribonucleic acid (DNA) molecule is the most compact and durable information storage medium in the world so far. Its high coding density and long-term preservation properties make itself one of the best data storage carriers for the future. High-throughput DNA synthesis is a key technology for “DNA data storage”, which encodes binary data stream (0/1) into quaternary long DNA sequences consisting of four bases (A/G/C/T). In this review, the workflow of DNA data storage and the basic methods of artificial DNA synthesis technology are outlined first. Then, the technical characteristics of different synthesis methods and the state-of-the-art of representative commercial companies, with a primary focus on silicon chip microarray-based synthesis and novel enzymatic DNA synthesis are presented. Finally, the recent status of DNA storage and new opportunities for future development in the field of high-throughput, large-scale DNA synthesis technology are summarized.</p>\",\"PeriodicalId\":68,\"journal\":{\"name\":\"Chemical Society Reviews\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":40.4000,\"publicationDate\":\"2024-03-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.rsc.org/en/content/articlepdf/2024/cs/d3cs00469d?page=search\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical Society Reviews\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/cs/d3cs00469d\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Society Reviews","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/cs/d3cs00469d","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
随着数字世界的爆炸式增长,预计到 2025 年,急剧增加的数据量将达到 175 ZB(1 ZB = 1012 GB)。存储如此庞大的全球数据将消耗大量资源。幸运的是,人们发现脱氧核糖核酸(DNA)分子是迄今为止世界上最紧凑、最耐用的信息存储介质。它的高编码密度和长期保存特性使其成为未来最好的数据存储载体之一。高通量 DNA 合成是 "DNA 数据存储 "的关键技术,它将二进制数据流(0/1)编码成由四个碱基(A/G/C/T)组成的四元长 DNA 序列。在这篇综述中,首先概述了 DNA 数据存储的工作流程和人工 DNA 合成技术的基本方法。然后,介绍了不同合成方法的技术特点和具有代表性的商业公司的先进技术,重点介绍了基于硅芯片的微阵列合成和新型酶法 DNA 合成。最后,总结了高通量、大规模 DNA 合成技术领域的 DNA 储存近况和未来发展的新机遇。
With the explosion of digital world, the dramatically increasing data volume is expected to reach 175 ZB (1 ZB = 1012 GB) in 2025. Storing such huge global data would consume tons of resources. Fortunately, it has been found that the deoxyribonucleic acid (DNA) molecule is the most compact and durable information storage medium in the world so far. Its high coding density and long-term preservation properties make itself one of the best data storage carriers for the future. High-throughput DNA synthesis is a key technology for “DNA data storage”, which encodes binary data stream (0/1) into quaternary long DNA sequences consisting of four bases (A/G/C/T). In this review, the workflow of DNA data storage and the basic methods of artificial DNA synthesis technology are outlined first. Then, the technical characteristics of different synthesis methods and the state-of-the-art of representative commercial companies, with a primary focus on silicon chip microarray-based synthesis and novel enzymatic DNA synthesis are presented. Finally, the recent status of DNA storage and new opportunities for future development in the field of high-throughput, large-scale DNA synthesis technology are summarized.
期刊介绍:
Chemical Society Reviews is published by: Royal Society of Chemistry.
Focus: Review articles on topics of current interest in chemistry;
Predecessors: Quarterly Reviews, Chemical Society (1947–1971);
Current title: Since 1971;
Impact factor: 60.615 (2021);
Themed issues: Occasional themed issues on new and emerging areas of research in the chemical sciences