Evaluating the effect of liposomes containing vitamin E on bone metabolism

To prevent causing pain during movement of the skeleton system, modern drug delivery systems use carriers to protect the drug, deliver it to the target site, and increase its efficiency. Liposomal encapsulation technology is one of the most promising carriers due to its biocompatibility, natural components, and cell membrane-like structure. In this study, liposomes containing vitamin E were used to increase osteoblast cells’ metabolism. Liposomes were prepared by hydrating a lipid film and loaded using a passive loading technique. DLS and zeta potential tests showed good stability and optimal size distribution. The cell culture results showed non-toxicity and improved bone cell growth. Alkaline phosphatase activity increased in the samples containing vitamin E. Samples were prepared in three DOPE: CHEMS ratios and sonication times. The best result was from the sample with a 4:1 ratio and 20-min sonication time, which showed the maximum vitamin E loading capacity.

Graphical abstract