Hyunwoo Kim, Seohyun Park, Rock Ki Kim, Yeong Ok Baik, Kyung Nam Kim, Duk Jae Oh
{"title":"开发和评估基于轨道摇摆运动的一次性使用细胞培养系统 CELBIC® 系统","authors":"Hyunwoo Kim, Seohyun Park, Rock Ki Kim, Yeong Ok Baik, Kyung Nam Kim, Duk Jae Oh","doi":"10.1007/s12257-024-00090-w","DOIUrl":null,"url":null,"abstract":"<p>The importance of a single-use bioreactor (SUB) is continuously increasing in research and industrial fields for biopharmaceutical production. In this study, a newly developed SUB system, the CELBIC<sup>®</sup> system, applied with the unique agitation method is introduced, and its physical properties and biological applications are evaluated. The mixing time was in the range of 11.0–129.0 s, and the volumetric mass transfer coefficient, <i>k</i><sub><i>L</i></sub><i>a</i>, measured through surface aeration, was in the range of 2.7–15.5 h<sup>−1</sup> at a working volume of 1–10 L. Biological evaluations using two Chinese hamster ovary (CHO) cell lines, CHO-DG44 and CHO-S cell, were carried out in the CELBIC<sup>®</sup> systems in 1 and 50 L scales, showing similar cell culture performance as that from stirred tank-type bioreactors. These results support the CELBIC<sup>®</sup> system as a new SUB system applicable to cell cultures for biopharmaceutical production.</p>","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development and evaluation of orbital rocking motion-based single-use cell culture system, the CELBIC® system\",\"authors\":\"Hyunwoo Kim, Seohyun Park, Rock Ki Kim, Yeong Ok Baik, Kyung Nam Kim, Duk Jae Oh\",\"doi\":\"10.1007/s12257-024-00090-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The importance of a single-use bioreactor (SUB) is continuously increasing in research and industrial fields for biopharmaceutical production. In this study, a newly developed SUB system, the CELBIC<sup>®</sup> system, applied with the unique agitation method is introduced, and its physical properties and biological applications are evaluated. The mixing time was in the range of 11.0–129.0 s, and the volumetric mass transfer coefficient, <i>k</i><sub><i>L</i></sub><i>a</i>, measured through surface aeration, was in the range of 2.7–15.5 h<sup>−1</sup> at a working volume of 1–10 L. Biological evaluations using two Chinese hamster ovary (CHO) cell lines, CHO-DG44 and CHO-S cell, were carried out in the CELBIC<sup>®</sup> systems in 1 and 50 L scales, showing similar cell culture performance as that from stirred tank-type bioreactors. These results support the CELBIC<sup>®</sup> system as a new SUB system applicable to cell cultures for biopharmaceutical production.</p>\",\"PeriodicalId\":8936,\"journal\":{\"name\":\"Biotechnology and Bioprocess Engineering\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-03-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotechnology and Bioprocess Engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s12257-024-00090-w\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology and Bioprocess Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s12257-024-00090-w","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Development and evaluation of orbital rocking motion-based single-use cell culture system, the CELBIC® system
The importance of a single-use bioreactor (SUB) is continuously increasing in research and industrial fields for biopharmaceutical production. In this study, a newly developed SUB system, the CELBIC® system, applied with the unique agitation method is introduced, and its physical properties and biological applications are evaluated. The mixing time was in the range of 11.0–129.0 s, and the volumetric mass transfer coefficient, kLa, measured through surface aeration, was in the range of 2.7–15.5 h−1 at a working volume of 1–10 L. Biological evaluations using two Chinese hamster ovary (CHO) cell lines, CHO-DG44 and CHO-S cell, were carried out in the CELBIC® systems in 1 and 50 L scales, showing similar cell culture performance as that from stirred tank-type bioreactors. These results support the CELBIC® system as a new SUB system applicable to cell cultures for biopharmaceutical production.
期刊介绍：
Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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