{"title":"荧光寿命成像显微镜仪器的进步：实现高速和三维","authors":"Jongchan Park, Liang Gao","doi":"10.1016/j.cossms.2024.101147","DOIUrl":null,"url":null,"abstract":"<div><p>Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool offering molecular specific insights into samples through the measurement of fluorescence decay time, with promising applications in diverse research fields. However, to acquire two-dimensional lifetime images, conventional FLIM relies on extensive scanning in both the spatial and temporal domain, resulting in much slower acquisition rates compared to intensity-based approaches. This problem is further magnified in three-dimensional imaging, as it necessitates additional scanning along the depth axis. Recent advancements have aimed to enhance the speed and three-dimensional imaging capabilities of FLIM. This review explores the progress made in addressing these challenges and discusses potential directions for future developments in FLIM instrumentation.</p></div>","PeriodicalId":295,"journal":{"name":"Current Opinion in Solid State & Materials Science","volume":"30 ","pages":"Article 101147"},"PeriodicalIF":12.2000,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1359028624000135/pdfft?md5=2d79ee53350f24617aa31efe1e6413b9&pid=1-s2.0-S1359028624000135-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Advancements in fluorescence lifetime imaging microscopy Instrumentation: Towards high speed and 3D\",\"authors\":\"Jongchan Park, Liang Gao\",\"doi\":\"10.1016/j.cossms.2024.101147\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool offering molecular specific insights into samples through the measurement of fluorescence decay time, with promising applications in diverse research fields. However, to acquire two-dimensional lifetime images, conventional FLIM relies on extensive scanning in both the spatial and temporal domain, resulting in much slower acquisition rates compared to intensity-based approaches. This problem is further magnified in three-dimensional imaging, as it necessitates additional scanning along the depth axis. Recent advancements have aimed to enhance the speed and three-dimensional imaging capabilities of FLIM. This review explores the progress made in addressing these challenges and discusses potential directions for future developments in FLIM instrumentation.</p></div>\",\"PeriodicalId\":295,\"journal\":{\"name\":\"Current Opinion in Solid State & Materials Science\",\"volume\":\"30 \",\"pages\":\"Article 101147\"},\"PeriodicalIF\":12.2000,\"publicationDate\":\"2024-03-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1359028624000135/pdfft?md5=2d79ee53350f24617aa31efe1e6413b9&pid=1-s2.0-S1359028624000135-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Opinion in Solid State & Materials Science\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1359028624000135\",\"RegionNum\":2,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Opinion in Solid State & Materials Science","FirstCategoryId":"88","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1359028624000135","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
Advancements in fluorescence lifetime imaging microscopy Instrumentation: Towards high speed and 3D
Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool offering molecular specific insights into samples through the measurement of fluorescence decay time, with promising applications in diverse research fields. However, to acquire two-dimensional lifetime images, conventional FLIM relies on extensive scanning in both the spatial and temporal domain, resulting in much slower acquisition rates compared to intensity-based approaches. This problem is further magnified in three-dimensional imaging, as it necessitates additional scanning along the depth axis. Recent advancements have aimed to enhance the speed and three-dimensional imaging capabilities of FLIM. This review explores the progress made in addressing these challenges and discusses potential directions for future developments in FLIM instrumentation.
期刊介绍：
Title: Current Opinion in Solid State & Materials Science
Journal Overview:
Aims to provide a snapshot of the latest research and advances in materials science
Publishes six issues per year, each containing reviews covering exciting and developing areas of materials science
Each issue comprises 2-3 sections of reviews commissioned by international researchers who are experts in their fields
Provides materials scientists with the opportunity to stay informed about current developments in their own and related areas of research
Promotes cross-fertilization of ideas across an increasingly interdisciplinary field
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