CRISPR 工程 B 细胞受体在体内的亲和成熟

IF 26.8 1区医学 Q1 ENGINEERING, BIOMEDICAL Nature Biomedical Engineering Pub Date : 2024-03-20 DOI:10.1038/s41551-024-01184-9

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引用次数: 0

摘要

利用CRISPR-Cas12a将原代B细胞中的小鼠抗体可变链基因直接替换为人类版本。经过编辑的细胞在体内进行了亲和性成熟，提高了 HIV-1 和 SARS-CoV-2 中和抗体的效力，同时不损失生物利用度。编辑细胞的亲和性成熟还有助于建立新的疫苗模型和适应性 B 细胞疗法。

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Affinity maturation of CRISPR-engineered B cell receptors in vivo

CRISPR–Cas12a was used to directly replace mouse antibody variable chain genes with human versions in primary B cells. The edited cells underwent affinity maturation in vivo, improving the potency of HIV-1 and SARS-CoV-2 neutralizing antibodies without loss of bioavailability. Affinity maturation of edited cells also enables new vaccine models and adaptive B cell therapies.

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来源期刊

Nature Biomedical Engineering Medicine-Medicine (miscellaneous)

CiteScore

45.30

自引率

1.10%

发文量

138

期刊介绍： Nature Biomedical Engineering is an online-only monthly journal that was launched in January 2017. It aims to publish original research, reviews, and commentary focusing on applied biomedicine and health technology. The journal targets a diverse audience, including life scientists who are involved in developing experimental or computational systems and methods to enhance our understanding of human physiology. It also covers biomedical researchers and engineers who are engaged in designing or optimizing therapies, assays, devices, or procedures for diagnosing or treating diseases. Additionally, clinicians, who make use of research outputs to evaluate patient health or administer therapy in various clinical settings and healthcare contexts, are also part of the target audience.