高压氧治疗对实验性卵巢扭转后脱体大鼠缺血再灌注损伤的影响

IF 0.8 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Diving and hyperbaric medicine Pub Date : 2024-03-31 DOI:10.28920/dhm54.1.16-22
Eralp Bulutlar, Ali Yilmaz, Gizem Berfin Uluutku Bulutlar, Yavuz Aslan, Hale Nur Bozdağ, Zafer Küçükodaci
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引用次数: 0

摘要

简介:本研究旨在探讨高压氧治疗(HBOT)能否改善卵巢扭转-脱位大鼠模型的缺血再灌注损伤:本研究旨在探讨高压氧治疗(HBOT)能否改善卵巢扭转-脱位大鼠模型的缺血-再灌注损伤:方法:27 只大鼠分为四组:手术假大鼠(S)(n = 6)接受与其他组(每组 n = 7)相同的麻醉和手术切口,但不对卵巢进行干预;扭转大鼠(T)接受开腹手术、卵巢扭转、再次开腹手术,并在三小时后牺牲;扭转和剥离大鼠(T/DT)进行开腹手术、卵巢扭转(三小时)、再剖腹和剥离,一周后处死;扭转、剥离、高压氧大鼠(T/DT/HBOT)进行开腹手术、卵巢扭转、再剖腹和剥离,一周后处死,期间提供 21 次高压氧(600 kPa 的 100% 氧气,50 分钟)。在所有组别中,牺牲后均采集血液以检测氧化应激或相关反应的标记物,并采集卵巢以进行组织学检查:结果:与 T/DT 组和 T/DT/HBOT 组相比,T/DT/HBOT 组的 8-羟基-2'-脱氧鸟苷(DNA 氧化损伤的标志物)和丙二醛(脂质过氧化的产物)水平较低。与 T/DT 组相比,T/DT/HBOT 组的抗苗勒氏管激素水平更高。此外,T/DT/HBOT 组的水肿、血管闭塞、中性粒细胞浸润和卵泡细胞损伤均少于 T/DT 组:综合评估生化和组织病理学结果,在这种卵巢扭转-扭转大鼠模型中,HBOT可减轻卵巢缺血/再灌注损伤。
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Effect of hyperbaric oxygen treatment on ischaemia-reperfusion injury in rats detorsioned after experimental ovarian torsion.

Introduction: This study aimed to investigate whether hyperbaric oxygen treatment (HBOT) could ameliorate ischaemia-reperfusion injury in a rat model of ovarian torsion-detorsion.

Methods: Twenty-seven rats were divided among four groups: surgical sham rats (S) (n = 6) underwent identical anaesthesia and surgical incisions to other groups (n = 7 per group) but with no ovary intervention; torsion rats (T) underwent laparotomy, ovarian torsion, relaparotomy and sacrifice after three hours; torsion and detorsion rats (T/DT) underwent laparotomy, ovarian torsion (three hours), relaparotomy and detorsion, and sacrifice after one week; torsion, detorsion, hyperbaric oxygen rats (T/DT/HBOT) underwent laparotomy, ovarian torsion, relaparotomy and detorsion, and sacrifice after one week during which HBOT was provided 21 times (100% oxygen at 600 kPa for 50 min). In all groups blood collection for markers of oxidative stress or related responses, and ovary collection for histology were performed after sacrifice.

Results: When the T/DT, and T/DT/HBOT groups were compared, 8-hydroxy-2'-deoxyguanosine (a marker of oxidative damage to DNA) and malondialdehyde (a product of lipid peroxidation) levels were lower in the T/DT/HBOT group. Anti-Mullerian hormone levels were higher in the T/DT/HBOT group compared to the T/DT group. In addition, oedema, vascular occlusion, neutrophilic infiltration and follicular cell damage were less in the T/DT/HBOT group than in the T/DT group.

Conclusions: When biochemical and histopathological findings were evaluated together, HBOT appeared reduce ovarian ischaemia / reperfusion injury in this rat model of ovarian torsion-detorsion.

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来源期刊
Diving and hyperbaric medicine
Diving and hyperbaric medicine 医学-公共卫生、环境卫生与职业卫生
CiteScore
1.70
自引率
22.20%
发文量
37
审稿时长
>12 weeks
期刊介绍: Diving and Hyperbaric Medicine (DHM) is the combined journal of the South Pacific Underwater Medicine Society (SPUMS) and the European Underwater and Baromedical Society (EUBS). It seeks to publish papers of high quality on all aspects of diving and hyperbaric medicine of interest to diving medical professionals, physicians of all specialties, scientists, members of the diving and hyperbaric industries, and divers. Manuscripts must be offered exclusively to Diving and Hyperbaric Medicine, unless clearly authenticated copyright exemption accompaniesthe manuscript. All manuscripts will be subject to peer review. Accepted contributions will also be subject to editing.
期刊最新文献
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