通过评估呼出气体中的挥发性有机化合物来检测艰难梭菌感染。

IF 3.7 4区 医学 Q1 BIOCHEMICAL RESEARCH METHODS Journal of breath research Pub Date : 2024-03-28 DOI:10.1088/1752-7163/ad3572
Teny M John, Nabin K Shrestha, Leen Hasan, Kirk Pappan, Owen Birch, David Grove, Billy Boyle, Max Allsworth, Priyanka Shrestha, Gary W Procop, Raed A Dweik
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引用次数: 0

摘要

艰难梭菌感染(CDI)是医院感染性腹泻的主要病因。目前诊断 CDI 的方法有其局限性:毒素的酶免疫测定灵敏度低,艰难梭菌 PCR 无法区分感染和定植。结合微生物因素、宿主因素以及宿主与微生物相互作用的理想诊断检测也许能确定真正感染的特征。评估呼出气体中的挥发性有机化合物(VOC)可能是鉴别 CDI 的有用检测方法。为了鉴定呼出气体中的多种挥发性有机化合物,我们使用热脱附-气相色谱-质谱法研究了 17 名 CDI 患者的呼气样本。年龄和性别相匹配的腹泻患者和艰难梭菌检测阴性(无 CDI)患者作为对照组。在测试的 65 种挥发性有机化合物中,9 种被用于建立一个二次判别模型,该模型的最终交叉验证准确率为 74%,灵敏度为 71%,特异性为 76%,曲线下接收器操作特征面积为 0.72。如果这些发现被更大规模的研究证实,呼气 VOC 分析可能会成为 CDI 的辅助诊断测试。
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Detection ofClostridioides difficileinfection by assessment of exhaled breath volatile organic compounds.

Clostridioides difficileinfection (CDI) is the leading cause of hospital-acquired infective diarrhea. Current methods for diagnosing CDI have limitations; enzyme immunoassays for toxin have low sensitivity andClostridioides difficilepolymerase chain reaction cannot differentiate infection from colonization. An ideal diagnostic test that incorporates microbial factors, host factors, and host-microbe interaction might characterize true infection. Assessing volatile organic compounds (VOCs) in exhaled breath may be a useful test for identifying CDI. To identify a wide selection of VOCs in exhaled breath, we used thermal desorption-gas chromatography-mass spectrometry to study breath samples from 17 patients with CDI. Age- and sex-matched patients with diarrhea and negativeC.difficiletesting (no CDI) were used as controls. Of the 65 VOCs tested, 9 were used to build a quadratic discriminant model that showed a final cross-validated accuracy of 74%, a sensitivity of 71%, a specificity of 76%, and a receiver operating characteristic area under the curve of 0.72. If these findings are proven by larger studies, breath VOC analysis may be a helpful adjunctive diagnostic test for CDI.

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来源期刊
Journal of breath research
Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM
CiteScore
7.60
自引率
21.10%
发文量
49
审稿时长
>12 weeks
期刊介绍: Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.
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