Shiwen Yuan, Cuicui Wang, Yanting Zeng, Jiawei Li, Weinian Li, Zhixiang He, Jinghua Ye, Fangfei Li, Yi Chen, Xiaojun Lin, Yan Xu, Na Yu, Xiaoyan Cai
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Sixty active SLE patients were selected, including 41 new-onset and 19 relapsing cases. One hundred healthy controls (HCs) were enrolled as the control group. Percentages of these cell subsets in SLE patients and HCs and their relationships with disease activity were analysed. Twenty-two of the 41 new-onset SLE patients were assessed before and after treatment. Changes in the frequencies of these cell subsets and their relationships with renal involvement were also analysed.</p><p><strong>Results: </strong>Compared with that in HCs, the percentage of total γδ-T cells among CD3<sup>+</sup> T cells in SLE patients was significantly lower. An imbalance in the proportions of Vδ1<sup>+</sup> and Vδ2<sup>+</sup> T cells among γδ-T cells was observed. The proportion of Vδ1<sup>+</sup> T cells among γδ-T cells was significantly greater in SLE patients than in HCs, while the proportion of Vδ2<sup>+</sup> T cells was significantly lower. Expression levels of PD-1, NKG2D, NKp30 and NKp46 in Vδ1<sup>+</sup> T cells and Vδ2<sup>+</sup> T cells from SLE patients were generally significantly increased, except for expression of NKG2D in Vδ2<sup>+</sup> T cells. Moreover, Vδ2<sup>+</sup> T cells, Vδ1<sup>+</sup> T cells and Vδ1<sup>+</sup>PD-1<sup>+</sup> T cells were associated with disease activity, and an increase in Vδ2<sup>+</sup> T-cell frequency and a decrease in PD-1 expression by γδ-T cells might be associated with effective treatment. Interestingly, our results indicated that Vδ2<sup>+</sup> T cells and their Vδ2<sup>+</sup>NKp30<sup>+</sup> T-cell subpopulation might be associated with renal involvement in SLE.</p><p><strong>Conclusion: </strong>A broad range of anomalies in the proportions of γδ-T-cell subsets and γδ-T cells in SLE patients may be involved in the pathogenesis of SLE. There is a strong association between Vδ2<sup>+</sup> T cells and their Vδ2<sup>+</sup>NKp30<sup>+</sup> T-cell subpopulation and LN occurrence. Our results indicate that γδ-T cells and their subpopulations might be key players in disease immunopathology and renal involvement in SLE.</p>","PeriodicalId":18044,"journal":{"name":"Lupus","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Aberrant phenotypes of circulating γδ-T cells may be involved in the onset of systemic lupus erythematosus.\",\"authors\":\"Shiwen Yuan, Cuicui Wang, Yanting Zeng, Jiawei Li, Weinian Li, Zhixiang He, Jinghua Ye, Fangfei Li, Yi Chen, Xiaojun Lin, Yan Xu, Na Yu, Xiaoyan Cai\",\"doi\":\"10.1177/09612033241240864\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Human gamma-delta T cells (γδ-T cells) play crucial roles in both innate and adaptive immune responses. 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Changes in the frequencies of these cell subsets and their relationships with renal involvement were also analysed.</p><p><strong>Results: </strong>Compared with that in HCs, the percentage of total γδ-T cells among CD3<sup>+</sup> T cells in SLE patients was significantly lower. An imbalance in the proportions of Vδ1<sup>+</sup> and Vδ2<sup>+</sup> T cells among γδ-T cells was observed. The proportion of Vδ1<sup>+</sup> T cells among γδ-T cells was significantly greater in SLE patients than in HCs, while the proportion of Vδ2<sup>+</sup> T cells was significantly lower. Expression levels of PD-1, NKG2D, NKp30 and NKp46 in Vδ1<sup>+</sup> T cells and Vδ2<sup>+</sup> T cells from SLE patients were generally significantly increased, except for expression of NKG2D in Vδ2<sup>+</sup> T cells. Moreover, Vδ2<sup>+</sup> T cells, Vδ1<sup>+</sup> T cells and Vδ1<sup>+</sup>PD-1<sup>+</sup> T cells were associated with disease activity, and an increase in Vδ2<sup>+</sup> T-cell frequency and a decrease in PD-1 expression by γδ-T cells might be associated with effective treatment. Interestingly, our results indicated that Vδ2<sup>+</sup> T cells and their Vδ2<sup>+</sup>NKp30<sup>+</sup> T-cell subpopulation might be associated with renal involvement in SLE.</p><p><strong>Conclusion: </strong>A broad range of anomalies in the proportions of γδ-T-cell subsets and γδ-T cells in SLE patients may be involved in the pathogenesis of SLE. There is a strong association between Vδ2<sup>+</sup> T cells and their Vδ2<sup>+</sup>NKp30<sup>+</sup> T-cell subpopulation and LN occurrence. Our results indicate that γδ-T cells and their subpopulations might be key players in disease immunopathology and renal involvement in SLE.</p>\",\"PeriodicalId\":18044,\"journal\":{\"name\":\"Lupus\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Lupus\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1177/09612033241240864\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/3/20 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"RHEUMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lupus","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/09612033241240864","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/20 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"RHEUMATOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
目的:人类γ-δT细胞(γδ-T细胞)在先天性免疫反应和适应性免疫反应中发挥着至关重要的作用。然而,人们对系统性红斑狼疮(SLE)患者体内γδT细胞的免疫状态知之甚少。本研究旨在探讨系统性红斑狼疮患者体内γδ-T细胞亚群的频率与疾病活动、自身抗体滴度和肾脏受累之间的潜在关系:通过流式细胞术鉴定循环中的γδ-T细胞及其亚群(根据表面受体(包括NKG2D、NKp30、NKp46和PD-1)的表达确定的Vδ1+ T细胞、Vδ2+ T细胞和γδ-T细胞亚群)。研究人员选择了 60 名活动性系统性红斑狼疮患者,其中包括 41 名新发患者和 19 名复发患者。100 名健康对照者(HCs)作为对照组。分析了这些细胞亚群在系统性红斑狼疮患者和健康对照组中的百分比及其与疾病活动的关系。对41名新发系统性红斑狼疮患者中的22人进行了治疗前后的评估。同时还分析了这些细胞亚群的频率变化及其与肾脏受累的关系:结果:与HCs相比,系统性红斑狼疮患者CD3+T细胞中总γδ-T细胞的比例明显降低。在γδ-T 细胞中,Vδ1+ 和 Vδ2+ T 细胞的比例失衡。系统性红斑狼疮患者γδ-T 细胞中 Vδ1+ T 细胞的比例明显高于 HCs,而 Vδ2+ T 细胞的比例则明显低于 HCs。系统性红斑狼疮患者的 Vδ1+ T 细胞和 Vδ2+ T 细胞中 PD-1、NKG2D、NKp30 和 NKp46 的表达水平普遍明显升高,只有 Vδ2+ T 细胞中 NKG2D 的表达水平升高。此外,Vδ2+ T细胞、Vδ1+ T细胞和Vδ1+PD-1+ T细胞与疾病活动性有关,Vδ2+ T细胞频率的增加和γδ-T细胞PD-1表达的减少可能与有效治疗有关。有趣的是,我们的研究结果表明,Vδ2+ T细胞及其Vδ2+NKp30+ T细胞亚群可能与系统性红斑狼疮的肾脏受累有关:结论:系统性红斑狼疮患者体内γδ-T细胞亚群和γδ-T细胞比例的各种异常可能与系统性红斑狼疮的发病机制有关。Vδ2+T细胞及其Vδ2+NKp30+T细胞亚群与LN的发生密切相关。我们的研究结果表明,γδ-T 细胞及其亚群可能是系统性红斑狼疮疾病免疫病理和肾脏受累的关键因素。
Aberrant phenotypes of circulating γδ-T cells may be involved in the onset of systemic lupus erythematosus.
Objective: Human gamma-delta T cells (γδ-T cells) play crucial roles in both innate and adaptive immune responses. However, much less is known about the immune status of γδT cells in systemic lupus erythematosus (SLE) patients. The objective of this study was to explore potential relationships between the frequency of γδ-T-cell subpopulations and disease activity, autoantibody titres and renal involvement in patients with SLE.
Methods: Circulating γδ-T cells and their subsets (Vδ1+ T cells, Vδ2+ T cells and γδ-T-cell subpopulations defined by expression of surface receptors, including NKG2D, NKp30, NKp46 and PD-1), were identified via flow cytometry. Sixty active SLE patients were selected, including 41 new-onset and 19 relapsing cases. One hundred healthy controls (HCs) were enrolled as the control group. Percentages of these cell subsets in SLE patients and HCs and their relationships with disease activity were analysed. Twenty-two of the 41 new-onset SLE patients were assessed before and after treatment. Changes in the frequencies of these cell subsets and their relationships with renal involvement were also analysed.
Results: Compared with that in HCs, the percentage of total γδ-T cells among CD3+ T cells in SLE patients was significantly lower. An imbalance in the proportions of Vδ1+ and Vδ2+ T cells among γδ-T cells was observed. The proportion of Vδ1+ T cells among γδ-T cells was significantly greater in SLE patients than in HCs, while the proportion of Vδ2+ T cells was significantly lower. Expression levels of PD-1, NKG2D, NKp30 and NKp46 in Vδ1+ T cells and Vδ2+ T cells from SLE patients were generally significantly increased, except for expression of NKG2D in Vδ2+ T cells. Moreover, Vδ2+ T cells, Vδ1+ T cells and Vδ1+PD-1+ T cells were associated with disease activity, and an increase in Vδ2+ T-cell frequency and a decrease in PD-1 expression by γδ-T cells might be associated with effective treatment. Interestingly, our results indicated that Vδ2+ T cells and their Vδ2+NKp30+ T-cell subpopulation might be associated with renal involvement in SLE.
Conclusion: A broad range of anomalies in the proportions of γδ-T-cell subsets and γδ-T cells in SLE patients may be involved in the pathogenesis of SLE. There is a strong association between Vδ2+ T cells and their Vδ2+NKp30+ T-cell subpopulation and LN occurrence. Our results indicate that γδ-T cells and their subpopulations might be key players in disease immunopathology and renal involvement in SLE.
期刊介绍:
The only fully peer reviewed international journal devoted exclusively to lupus (and related disease) research. Lupus includes the most promising new clinical and laboratory-based studies from leading specialists in all lupus-related disciplines. Invaluable reading, with extended coverage, lupus-related disciplines include: Rheumatology, Dermatology, Immunology, Obstetrics, Psychiatry and Cardiovascular Research…