在马蹄莲提取物中绿色合成银和金纳米粒子及其抗菌活性。

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioprocess and Biosystems Engineering Pub Date : 2024-08-01 Epub Date: 2024-03-21 DOI:10.1007/s00449-024-02994-6
Shahid Khan, Abdur Rauf, Abdullah S M Aljohani, Yahya S Al-Awthan, Zubair Ahmad, Omar S Bahattab, Shehla Khan, Muhammad Saadiq, Shahid Ali Khan, Rekha Thiruvengadam, Muthu Thiruvengadam
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引用次数: 0

摘要

在本研究中,选择了瓶子草[Callistemon viminalis (Sol. ex Gaertn.) G. Don]植物用于银(Ag)和金(Au)纳米粒子的绿色合成,并评估其抗菌和抗真菌活性。C. viminalis 的植物化学筛选证实了生物碱、蒽醌类、皂苷、单宁酸、贝特矢车菊素、酞檀素、香豆素、萜类、甾体、苷类和蛋白质的存在。为了表征合成的银和金 NPs,我们使用了紫外可见光谱、傅立叶变换红外光谱来鉴定官能团,使用场发射扫描电子显微镜(FE-SEM)来测量粒度,并使用 EDX 进行元素分析。绿色合成的银纳米颗粒和金纳米颗粒的紫外可见吸收光谱显示,银纳米颗粒的最大吸收带在 420 纳米处,金纳米颗粒的最大吸收带在 525 纳米处。对合成的 NPs 进行的 FE-SEM 分析表明,它们呈圆形,大小为 100 nm。对合成的银纳米粒子和金纳米粒子进行了元素分析,证实了纳米粒子的纯度。此外,还对绿色合成的银和金纳米粒子进行了抗菌和抗真菌活性评估,结果表明它们对大肠杆菌、金黄色葡萄球菌、肺炎克雷伯菌、绿脓杆菌、白色念珠菌、克鲁西菌、曲霉菌和毛霉菌具有显著的抑制活性。Ag NPs 对大肠杆菌和铜绿假单胞菌的最大抑菌区分别为 15.5 ± 0.75 毫米和 15 ± 0.85 毫米。同样,Ag NPs 对毛霉菌和曲霉菌的抑制面积分别达到 13.5 ± 0.95 毫米和 13 ± 0.70 毫米。这项工作将为利用绿色化学开发新型抗菌剂打开大门。
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Green synthesis of silver and gold nanoparticles in Callistemon viminalis extracts and their antimicrobial activities.

In the current study, the bottlebrush [Callistemon viminalis (Sol. ex Gaertn.) G. Don] plant was selected for the green synthesis of silver (Ag) and gold (Au) nanoparticles and to evaluate its antibacterial and antifungal activities. Phytochemical screening of C. viminalis confirmed the presence of alkaloids, anthraquinones, saponins, tannins, betacyanins, phlobatanins, coumarins, terpenoids, steroids, glycosides, and proteins. To characterize the synthesized Ag and Au NPs, UV-Visible spectroscopy, FTIR spectroscopy for functional group identification, field emission scanning electron microscopy (FE-SEM) for particle size, and elemental analysis were performed using EDX. The UV-Visible absorption spectra of the green-synthesized Ag and Au nanoparticles were found to have a maximum absorption band at 420 nm for Ag NPs and 525 nm for Au NPs. FE-SEM analysis of the synthesized NPs revealed a circular shape with a size of 100 nm. Elemental analysis was performed for the synthesis of Ag and Au NPs, which confirmed the purity of the nanoparticles. The greenly synthesized Ag and Au NPs were also evaluated for their anti-bacterial and anti-fungal activities, which exhibited prominent inhibition activities against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Candida albicans, C. krusei, Aspergillus sp., and Trichoderma species. The highest zone of inhibition 15.5 ± 0.75 and 15 ± 0.85 mm was observed for Ag NPs against E. coli and P. aeruginosa. Similarly, Trichoderma sp. and Aspergillus sp. were inhibited by Ag NPs up to 13.5 ± 0.95 and 13 ± 0.70 mm. This work will open doors for the development of new antimicrobial agents using green chemistry.

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来源期刊
Bioprocess and Biosystems Engineering
Bioprocess and Biosystems Engineering 工程技术-工程:化工
CiteScore
7.90
自引率
2.60%
发文量
147
审稿时长
2.6 months
期刊介绍: Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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