基于移动遗传因子的基因编辑和基因组工程:最新进展与应用。

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology advances Pub Date : 2024-03-22 DOI:10.1016/j.biotechadv.2024.108343
Jaeseong Hwang , Dae-Yeol Ye , Gyoo Yeol Jung , Sungho Jang
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引用次数: 0

摘要

基因组工程给多个科学领域带来了革命性的变化,从生物化学和基础研究到治疗用途和作物开发,不一而足。目前已开发出多种工程工具包,用于有效修改生物体的基因组序列。然而,基于移动遗传因子(MGEs)的基因组工程技术缺乏广泛的综述,这种技术通过改变遗传因子在基因组中的位置,诱导宿主细胞内的遗传多样性。本综述全面介绍了 MGEs 作为强大基因组工程工具的多功能性,为解决基因组工程相关挑战提供了有效的解决方案。MGEs包括DNA转座子、反转座子、反转座子和CRISPR相关转座子,具有多种优势,如宿主范围广、全基因组诱变、高效的大尺寸DNA整合、多重能力和原位单链DNA生成。我们重点介绍了每种基于 MGE 工具的组成部分、机制和特点,以突出它们在细胞中的应用。最后,我们讨论了基于 MGE 的基因组工程目前面临的挑战,并对这一变革性技术不断发展的前景提出了见解。总之,基因组工程与 MGE 的结合在应对各种挑战和推动基因操作领域的发展方面展现出了巨大的潜力,有望彻底改变我们设计和理解各种生物基因组的能力。
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Mobile genetic element-based gene editing and genome engineering: Recent advances and applications

Genome engineering has revolutionized several scientific fields, ranging from biochemistry and fundamental research to therapeutic uses and crop development. Diverse engineering toolkits have been developed and used to effectively modify the genome sequences of organisms. However, there is a lack of extensive reviews on genome engineering technologies based on mobile genetic elements (MGEs), which induce genetic diversity within host cells by changing their locations in the genome. This review provides a comprehensive update on the versatility of MGEs as powerful genome engineering tools that offers efficient solutions to challenges associated with genome engineering. MGEs, including DNA transposons, retrotransposons, retrons, and CRISPR-associated transposons, offer various advantages, such as a broad host range, genome-wide mutagenesis, efficient large-size DNA integration, multiplexing capabilities, and in situ single-stranded DNA generation. We focused on the components, mechanisms, and features of each MGE-based tool to highlight their cellular applications. Finally, we discussed the current challenges of MGE-based genome engineering and provided insights into the evolving landscape of this transformative technology. In conclusion, the combination of genome engineering with MGE demonstrates remarkable potential for addressing various challenges and advancing the field of genetic manipulation, and promises to revolutionize our ability to engineer and understand the genomes of diverse organisms.

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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
期刊最新文献
A non-classical view of antibody properties: Allosteric effect between variable and constant regions. Expanding the frontiers of genome engineering: A comprehensive review of CRISPR-associated transposons. Advances and challenges in preparing membrane proteins for native mass spectrometry Leveraging independent component analysis to unravel transcriptional regulatory networks: A critical review and future directions. Recent advances in culture medium design for enhanced production of monoclonal antibodies in CHO cells: A comparative study of machine learning and systems biology approaches.
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