使用密度测定 KLT 法对班丹县流通的尿酸草药制剂中的药用化学品 (BKO) 进行鉴定测试

M. Yusril, Virsa Handayani, Selpida Handayani
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摘要

中药中的药用化学品(BKO)会对健康不利。尿酸中药中常添加的一些 BKO 如吡罗昔康,吡罗昔康是一种用于治疗痛风的药物。但实际上,市场上仍有不少含有 BKO 的中药。添加的药用化学物质会对消费者产生有害的副作用。本研究对尿酸中药中的吡罗昔康进行了鉴定。鉴定是通过感官/宏观和显微测试形式的初步测试进行的。采用薄层色谱法(KLT)进行定性检测,采用密度计进行定量检测。感官测试结果显示,样品的粉末和胶囊剂型呈深灰色、白色、黄色和淡黄色。它有苦味和芳香气味。显微测试结果显示,减肥药材成分中所含的每种成分都有植物碎片。在流动相丙酮:氯丙酮:正己烷(7:2:1)的 KLT 试验中,吡罗昔康对比剂的 Rf 值为 0.8。对五个样品的分析表明,其 Rf 值与吡罗昔康对比剂相同。对样品进行的密度测试表明,1、2 和 4 号样品对吡罗昔康呈阳性反应。
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Identification Test of Medicinal Chemicals (BKO) in Uric Acid Herbal Preparations Circulating in Bantaeng District Using the Densitometric KLT Method
Medicinal Chemicals (BKO) in herbal medicine will be bad for health. Some of the BKOs commonly added to uric acid herbs such as piroxicam, piroxicam is a drug used for the treatment of gout. But the reality is that in the market there are still many that contain BKO. Added medicinal chemicals can have harmful side effects for consumers. In this study, the identification of piroxicam in uric acid herbal medicine has been carried out. Identification was carried out by preliminary tests in the form of organoleptic / macroscopic and microscopic tests. Qualitative test in the form of thin layer chromatography (KLT), and quantitative test in the form of densitometry. The organoleptic test results showed that the samples had powder and capsule dosage forms with dark ash, white, yellow and pale yellow colors. It has a bitter taste and aromatic odor. Microscopic test results showed plant fragments from each ingredient contained in the composition of slimming herbs. In the KLT test with the mobile phase acetone: chlorophom: n-hexan (7: 2: 1) obtained the Rf value of piroxicam comparator which is 0.8. Analysis of the five samples showed the same Rf value as the piroxicam comparator. Densitometry test on the samples showed that samples 1, 2 and 4 were positive for piroxicam.
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