为热带醋酸杆菌微生物发酵甘油生产甘油酸的 1m3 容积生物反应器设计搅拌系统。

Daniel Cabrera-Valle, Henry Pérez
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引用次数: 0

摘要

本研究为一个 1m3 容积的生物反应器设计了一套搅拌系统,该反应器旨在以 20% v/v 的甘油为主要底物,通过热带酵母菌的微生物发酵生产甘油酸 (GA)。以前的研究评估了甘油酸生产的理想条件,在此基础上,利用初始数据确定了叶轮类型,并进行了后续计算,包括叶轮雷诺数、功耗和搅拌时间。一旦确定了搅拌器类型,就可以利用标准几何关系来确定搅拌系统各组件的尺寸,例如罐体尺寸、挡板和搅拌器尺寸。功率消耗计算与其他流变特性类似的发酵介质研究结果进行了比较。在搅拌速度为 500 转/分钟的情况下,搅拌器搅拌 1 立方米培养基的耗电量为 8.35 千瓦,在 5.14 秒内实现 95% 的均质化,搅拌时间误差约为 10%。关键词:搅拌系统;甘油酸;生物反应器
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Diseño de un sistema de agitación para un biorreactor de 1m3 de capacidad para la producción de ácido glicérico a partir de glicerol por fermentación microbiana de Acetobacter tropicalis
This work presents the design of an agitation system for a 1m3 capacity bioreactor intended to produce glyceric acid (GA) through microbial fermentation of A. tropicalis using 20% v/v glycerol as the primary substrate. Building on previous studies that evaluated ideal conditions for glyceric acid production, initial data were utilized to identify the impeller type and perform subsequent calculations, including impeller Reynolds number, power consumption, and mixing time. Once the agitator type was specified, standard geometric relationships were employed to size components of the agitation system, such as tank size, baffle plates, and agitator dimensions. Power consumption calculations were compared with results from other studies on fermentation media with similar rheological characteristics. The agitator's power consumption for mixing 1m3 of medium was determined to be 8.35 kW, achieving 95% homogenization in 5.14 seconds, with a mixing time error of approximately 10%, operating at an agitation speed of 500 rpm. Keywords: agitation system; glyceryc acid; bioreactor
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