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HIV knowledge and preventive Standards Precautions Among Healthcare Workers in Blood Transfusion Centers 输血中心医护人员的艾滋病知识和预防性标准预防措施
Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.44
Nasir Muwfaq Younis, Mahmoud Mohammed Ahmed, Alaa Yousif Ayed
The number of instances of HIV has climbed considerably in the previous ten years, which is cause for alarm.Because healthcare workers are future healthcare professionals, they must have proper knowledge and preventive standards and precautions about HIV because they will play a vital role in the prevention of HIVtransmission and treatment of HIV patients. The article aims to identify HIV knowledge and preventive standard precautions among healthcare workers in blood transfusion Centers. Cross-sectional descriptive researchto characterize and provide information about the knowledge and preventive standards precautions amonghealthcare workers in blood transfusion Centers from March 25 to April 15, 2022. Participants from the described Studies (N = 34) provided data for these analyses. This result revealed that the participants' knowledgeranged between acceptable and good (47.05 and 52.95). Also, participants' knowledge of prevention strategieswas similar, but the hand hygiene strategy was the most valuable (M: 3.62 and SD: 0.45). The instrumentconsists of 3 parts: part 1: demographical variables. Part two: healthcare workers' knowledge of HIV, Part 3:preventive standards precautions among healthcare workers. The current study of healthcare personnel showsthat they have a decent understanding of HIV based on the precise results and adhere to the preventivemeasures during the blood transfusion.Keywords: HIV, knowledge, preventive Standards precautions, Healthcare Workers, Blood Transfusion Centers
由于医护人员是未来的医护专业人员,他们必须掌握有关艾滋病的正确知识、预防标准和预防措施,因为他们将在预防艾滋病传播和治疗艾滋病患者方面发挥重要作用。本文旨在了解输血中心医护人员的艾滋病知识和预防标准。从 2022 年 3 月 25 日至 4 月 15 日,对输血中心的医护人员进行了横断面描述性研究,以描述和提供有关输血中心医护人员的艾滋病知识和预防标准预防措施的信息。上述研究的参与者(N = 34)为这些分析提供了数据。结果显示,参与者的知识水平介于可接受和良好之间(47.05 和 52.95)。此外,参与者对预防策略的了解程度相似,但手卫生策略最有价值(中值:3.62,标度:0.45)。问卷由三部分组成:第一部分:人口统计学变量。第二部分:医护人员对艾滋病的了解;第三部分:医护人员的预防标准。目前对医护人员的研究表明,根据精确的结果,他们对 HIV 有一定的了解,并在输血过程中遵守预防措施:HIV;知识;预防标准;医护人员;输血中心
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引用次数: 0
Effect of injection with microelements, jasmonic and citric acid on some date palm c.v Khastawi yieldtraits 注射微量元素、茉莉酸和柠檬酸对某些椰枣产量特征的影响
Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.53
W. Taha, Kh.A.S. Al-Hamdani, KH. N. AL-Assal
The study was conducted at the Ishaqi palm plant affiliated with the Department of Horticulture/Ministry ofAgriculture during the growing season of 2021 on date palm trees of the Khastawi variety to study the responseof the yield traits of the Khastawi date palm trees to the injection of microelements, jasmonic and citric acid.The study included three factors: The first factor is the injection of microelements with three treatments: injection with water only, symbolized by D0, iron injection at a concentration of 300 mg, and iron sulfate. Palm1, symbolized by D1, and boron injection through boric acid at a concentration of 40 mg Palm-1, symbolizedby D2. The second factor is the treatment of injections with the Jasmonic growth regulator, which was injectedthree days after the injection with the microelements and with the same number of additions (0, 50, 100)micrograms. L-1 is symbolized by G0, G1, and G2 sequentially. The trees were injected with the microelementsand the Jasmonic growth regulator before Immunization, after vaccination in 20 days, and after 40 days. Thethird factor was injection with citric acid and included three levels (0, 500, 1000) mg l-1 symbolized by S0, S1,and S2 sequentially. The results showed that the D2 treatment was significantly superior in the average diameter, length, size and weight of the fruit and the total yield, as it reached 24.86 mm, 3.46 cm, 11.10 cm, 10.07g, 36.00 kg.palm-1 sequentially, while the comparison treatment gave the lowest average for the traits. As forthe effect of jasmonic acid, the injection treatment was superior to (100 µg L-1). (G2) gave the highest averagefor the diameter, length, size, weight of the fruit, and the total yield, which reached 25.90 mm, 3.63 cm, 12.09cm, 10.93 g, and 35.96 kg. Palm-1, respectively, while the comparison treatment gave the lowest average forthe traits above as for the effect of citric acid. The treatment exceeded 1000 mg.L-1 (S2) gave the highest ratein the diameter, length, size, and weight of the fruit, and the total yield, reaching 24.84 mm, 3.49 cm, 11.13cm, 9.04 g, and 36.30 kg. Palm-1, respectively, while the comparison treatment gave the lowest average forthe traits above, as the two- and three-interactions showed. The research factors have significant differencesfor all the studied traits.Keywords: Trace elements, jasmonic acid, citric acid, Khastawi variety.
这项研究于 2021 年生长季节在农业部园艺司下属的伊沙奇棕榈种植园进行,对象是哈斯塔维(Khastawi)枣树,目的是研究哈斯塔维(Khastawi)枣树的产量性状对微量元素、茉莉酸和柠檬酸注射的反应:研究包括三个因素:第一个因素是微量元素的注射,有三种处理方法:仅用水注射(用 D0 表示)、注射 300 毫克浓度的铁和硫酸铁。以 D1 表示,通过硼酸注入浓度为 40 毫克(Palm-1)的硼,以 D2 表示。第二个因素是茉莉生长调节剂的注射处理,在注射微量元素三天后注射,添加量相同(0、50、100)微克。L-1依次用 G0、G1 和 G2 表示。苗木在免疫前、免疫后 20 天和 40 天内分别注射了微量元素和茉莉生长调节剂。第三个因素是注射柠檬酸,包括三个水平(0、500、1000)毫克升-1,依次用 S0、S1 和 S2 表示。结果表明,D2 处理在果实的平均直径、长度、大小、重量和总产量方面明显优于其他处理,依次达到 24.86 毫米、3.46 厘米、11.10 厘米、10.07 克、36.00 千克/棕榈-1,而对比处理的平均性状最低。至于茉莉酸的效果,注射处理优于(100 µg L-1)。(G2)的果实直径、长度、大小、重量和总产量的平均值最高,分别达到 25.90 毫米、3.63 厘米、12.09 厘米、10.93 克和 35.96 千克。就柠檬酸的影响而言,对比处理的上述性状平均值最低。超过 1000 mg.L-1 的处理(S2)在果实直径、长度、大小、重量和总产量方面的比率最高,分别达到 24.84 mm、3.49 cm、11.13 cm、9.04 g 和 36.30 kg/Palm-1。而对比处理在上述性状上的平均值最低,这也是两两交互作用和三两交互作用的结果。研究因子对所有研究性状都有显著差异:微量元素 茉莉酸 柠檬酸 哈斯塔维品种
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引用次数: 0
Root-shoot ratio and its relationships with physiological characteristics, growth and biomass yield ofGynura procumbens under different shade levels and plant density 不同遮荫水平和植株密度下根-芽比及其与玄参生理特征、生长和生物量产量的关系
Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.52
Omar Ali Ahmed, Martini Mohammad Yusoff, A. Misran, Puteri Edaroyati Megat Wahab, Qusay Abdualhamza Muttaleb
Gynura procumbens is one of the most common medicinal plants in the Asteraceae family, with extensivepharmacological properties. The experiment was conducted to evaluate the effects of different shade levels (0and 30% shade) and plant density (9, 15, and 25 plants m-2) on root-shoot ratio and it is a relationship withphysiology, growth, and biomass yield using split-plot design with three replications. Increasing shade levelto 30% shade significantly decreased root-shoot ratio (RSR) by22.54%, while total leaf dry weight per plant(TLDW) and total leaf dry weight per square meter (TLDW m-2) increased by 35.64, 11.58, and 32.18%,respectively due to negative correlation with RSR. Increasing plant density from 9 to 25 plants m-2 significantly increased RSR and TLDW m-2 by 67.71 and 18.54%, respectively, while TLDW decreased by 57.31%.There was a negative correlation between RSR and biomass yield per plant. Under stressed conditions (fullsunlight and high plant density), G. procumbent plants appeared to change strategy to absorb limited resources,allocate more biomass to the root system, and reduce aboveground parts' size to survive, resulting in highRSR.Keywords: Gynura procumbens, shade, plant density, root-shoot ratio, physiology, growth, biomass
银胶菊(Gynura procumbens)是菊科植物中最常见的药用植物之一,具有广泛的药理作用。实验采用三次重复的小区设计,评估了不同遮荫度(0% 和 30%)和植株密度(9、15 和 25 株 m-2)对根-芽比的影响,以及根-芽比与生理、生长和生物量产量的关系。遮荫度提高到 30% 时,根芽比(RSR)显著降低了 22.54%,而每株总叶干重(TLDW)和每平方米总叶干重(TLDW m-2)分别增加了 35.64%、11.58% 和 32.18%,与 RSR 呈负相关。植株密度从 9 株 m-2 增加到 25 株 m-2 后,RSR 和 TLDW m-2 分别显著增加了 67.71% 和 18.54%,而 TLDW 则减少了 57.31%。在胁迫条件下(全日照和高植株密度),原生植物似乎改变了吸收有限资源的策略,将更多的生物量分配给根系,并缩小地上部分的体积以求生存,从而导致高RSR:平卧草 Gynura procumbens 遮荫 植物密度 根-芽比 生理 生长 生物量
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引用次数: 0
Coexistencia de procesos productivos y de conservación en la Amazonía ecuatoriana 厄瓜多尔亚马逊河流域生产与保护进程的共存
Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.1
Maritza Sánchez-Capa, Hilda Fabiola Anguaya Isama
La región amazónica de Ecuador está conforma por las provincias Sucumbíos, Orellana, Napo, Pastaza, Morona Santiago y Zamora Chinchipe, su extensión es de ~120.000 Km2 1. En las dos últimas décadas, la educación superior se ha implementado en cada una de las provincias, buscando responder a las necesidades de profesionales en los diferentes sectores que constituyen las actividades económicas principales de cada provincia. Sucumbíos y Orellana son provincias que sustentan su economía en la producción petrolera, sin embargo, en esta superficie se encuentran áreas protegidas como la reserva de la biósfera YASUNÍ, parque nacional Cuyabeno, reserva Limoncocha, que son áreas con una gran biodiversidad por lo que la coexistencia entre conservación y producción industrial generan una búsqueda consante de alternativas sustentables y sostenibles
厄瓜多尔的亚马逊地区由苏昆毕奥斯省、奥雷亚纳省、纳波省、帕斯塔萨省、莫罗纳-圣地亚哥省和萨莫拉-钦奇佩省组成,面积约为12万平方公里1。 在过去的二十年中,各省都开展了高等教育,以满足构成各省主要经济活动的不同行业对专业人才的需求。苏昆毕奥斯省和奥雷亚纳省的经济以石油生产为基础;然而,亚苏尼(YASUNÍ)生物圈保护区、库亚贝诺(Cuyabeno)国家公园和利蒙科查(Limoncocha)保护区等保护区都位于该地区,这些地区具有丰富的生物多样性,因此,保护与工业生产的共存促使人们不断寻求可持续和可持续的替代方案。
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引用次数: 0
Predictive Model in Production through Progressive Web Applications to Forecast Moniliasis in Cacao. 通过渐进式网络应用程序建立生产中的预测模型,以预测可可中的莫尼里斯病。
Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.14
Aracely Miranda, Byron Bonifaz, Wilson Chango, Pedro Aguilar
Cocoa is considered a significant crop in Ecuador, as it represents a favorable source of income for the country's economy, thanks to the remarkable quality of the product. However, it faces a significant issue in its crops: moniliasis, a fungal disease that attacks cocoa cultivation, is present in most Latin American countries. Consequently, this leads to decreased cocoa production and a lower final product quality. The study focuses on designing a predictive production model through a progressive web application to forecast moniliasis in cocoa. The objective is to create an application that anticipates the presence of this disease, thereby contributing to the improvement of the local economy for all farmers. Various methodologies were employed, including bibliographic methods, design science research methodology, and machine learning models. The results obtained from this research indicate that the Gradient Boosting Classifier is the algorithm that best fits the provided dataset. Once this algorithm was identified, a progressive web application was developed and made available for public use by farmers. Furthermore, the efficiency of the predictive model was verified using the statistical method of central tendency, demonstrating that the predictive model is beneficial, primarily by saving farmers a significant amount of time. Anticipating the disease enables timely preventive and corrective measures, which could reduce losses in cocoa production and enhance the quality of the final product. Keywords: cacao, moniliasis, predictive model, progressive web apps, supervised learning.
可可被认为是厄瓜多尔的重要作物,因为可可产品的卓越品质为国家经济提供了有利的收入来源。然而,厄瓜多尔的可可种植却面临着一个重大问题:在大多数拉丁美洲国家,可可种植中都存在一种真菌性疾病--单孢菌病。因此,这导致可可产量下降,最终产品质量降低。本研究的重点是通过渐进式网络应用程序设计一个预测性生产模型,以预测可可中的单胞菌病。目的是创建一个能预测这种疾病出现的应用程序,从而为改善所有农民的当地经济做出贡献。研究采用了多种方法,包括文献学方法、设计科学研究方法和机器学习模型。研究结果表明,梯度提升分类器是最适合所提供数据集的算法。一旦确定了这一算法,就开发了一个渐进式网络应用程序,供农民公开使用。此外,使用中心倾向统计方法验证了预测模型的效率,证明该预测模型是有益的,主要是为农民节省了大量时间。通过预测病害,可以及时采取预防和纠正措施,从而减少可可生产中的损失,提高最终产品的质量。关键词:可可、单核细胞增多症、预测模型、渐进式网络应用程序、监督学习。
{"title":"Predictive Model in Production through Progressive Web Applications to Forecast Moniliasis in Cacao.","authors":"Aracely Miranda, Byron Bonifaz, Wilson Chango, Pedro Aguilar","doi":"10.21931/rb/2024.09.01.14","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.14","url":null,"abstract":"Cocoa is considered a significant crop in Ecuador, as it represents a favorable source of income for the country's economy, thanks to the remarkable quality of the product. However, it faces a significant issue in its crops: moniliasis, a fungal disease that attacks cocoa cultivation, is present in most Latin American countries. Consequently, this leads to decreased cocoa production and a lower final product quality. The study focuses on designing a predictive production model through a progressive web application to forecast moniliasis in cocoa. The objective is to create an application that anticipates the presence of this disease, thereby contributing to the improvement of the local economy for all farmers. Various methodologies were employed, including bibliographic methods, design science research methodology, and machine learning models. The results obtained from this research indicate that the Gradient Boosting Classifier is the algorithm that best fits the provided dataset. Once this algorithm was identified, a progressive web application was developed and made available for public use by farmers. Furthermore, the efficiency of the predictive model was verified using the statistical method of central tendency, demonstrating that the predictive model is beneficial, primarily by saving farmers a significant amount of time. Anticipating the disease enables timely preventive and corrective measures, which could reduce losses in cocoa production and enhance the quality of the final product.\u0000 \u0000Keywords: cacao, moniliasis, predictive model, progressive web apps, supervised learning.","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140241233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heart failure prognostic algorithm using Spectral Analysis and MATLAB software. 使用频谱分析和 MATLAB 软件的心力衰竭预后算法。
Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.12
Segundo Fabián Siza Moposita, Miguel Ángel Sáez Paguay, Yesenia Maricela Fiallos Godoy, Ricardo Fabián Siza Gualpa
Fourier analysis for biological signals is based on the use of the infinite sum of sines and cosines that allows modeling: the periodic functioning of the heart, its amplitude, frequency, and phase period, transforming these signals into images called ECG, based on studies and programs that model the ideal functioning of the heart. In this work, a mathematical algorithm has been designed to predict the cardiac pathology called bradycardia, which relates the prolongation of the monthly QT interval in the order of 10-4 seconds/month in a time of 10 years with the ventricular alteration. MATLAB software and spectral analysis are used to contrast the spectrum without pathology, which contains harmonics of greater amplitude, with a spectrum already with pathology that reaches heart failure, where the most significant number of harmonics are grouped in the first values, and then the model with an exponential function the delay of the QT interval in the ECG, concluding that up to 40 months after the onset of the pathology, the patient can counteract the disease. In comparison, by 80 months, difficulties arise, even the disease becomes irreversible in the last months, and the blood-propelling organ ceases to function.Keywords: Heart Failure Prognostic, Bradycardia, Fourier Series, Spectral Analysis.
生物信号的傅立叶分析是基于正弦和余弦的无限和,它可以模拟:心脏的周期性运转、振幅、频率和相位周期,根据模拟心脏理想运转的研究和程序,将这些信号转换成称为心电图的图像。在这项工作中,我们设计了一种数学算法来预测被称为心动过缓的心脏病变,这种病变与 10 年内每月 QT 间期的延长(10-4 秒/月)和心室变化有关。利用 MATLAB 软件和频谱分析,将无病变的频谱(其中包含振幅较大的谐波)与已出现病变、达到心力衰竭的频谱(其中最重要的谐波数都集中在第一组值中)进行对比,然后利用指数函数模型对心电图中的 QT 间期延迟进行分析,得出结论认为,在病变发生后的 40 个月内,患者可以对抗疾病。相比之下,到了80个月,困难就会出现,甚至疾病在最后几个月变得不可逆转,血液推进器官停止运作:心衰预后 心动过缓 傅立叶序列 频谱分析
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引用次数: 0
Análisis multitemporal de deforestación y cambio de la cobertura del suelo, en el cantón La Joya de los Sachas, período 1990-2018. 1990-2018 年期间拉霍亚德洛斯萨夏斯州森林砍伐和土地覆被变化的多时空分析。
Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.18
Fernando Paredes-Arcos, Leider Tinoco-Jaramillo, Carlos D. Congo-Yépez, Yadira Vargas-Tierras
La Joya de los Sachas, es uno de los principales cantones productores de petróleo y productos agrícolas; sin embargo, el incremento de la frontera agrícola ha ocasionado cambios importantes en la cobertura y uso del suelo. Ante esta situación, el estudio tuvo la finalidad de conocer las causas y efectos que ha ocasionado el cambio de cobertura para obtener las tasas anuales e índices de cambio de cobertura. El análisis se realizó mediante el geoprocesamiento de información geográfica disponible en el portal del Ministerio de Ambiente Agua y Transición Ecológica (MAATE) y el Instituto Geográfico Militar (IGM), se utilizó el método de tablas de tabulación cruzada y sobreposición de capas para determinar el contraste de las coberturas entre las fechas establecidas. Los resultados muestran un incremento acumulado de 17.626,5 ha de tierra agrícola y 16.767,6 ha de pasto en los últimos 28 años; las actividades agropecuarias se han convertido en la principal causa de deforestación. Durante el período 1990-2018 se perdieron 36.413 ha de bosques, con una tasa anual de deforestación de 1.300 ha*año-1. Este comportamiento permitió estimar si la tendencia persiste, en 30 años aproximadamente La Joya de los Sachas perderá completamente el bosque. Además, las políticas públicas, dolarización, apertura de caminos y cambios en la actividad agrícola causaron impactos en las coberturas y usos de suelo durante los tres periodos evaluados (1990-2000, 2000-2008 y 2008-2018). Palabras Clave: Suelo, Uso de suelo, cobertura, tierra agrícola, pastizal, bosque, mapas.
La Joya de los Sachas 是主要的石油和农产品产区之一;然而,农业边界的扩大导致土地覆被和土地利用发生了重大变化。鉴于这种情况,研究的目的是确定土地覆被变化的原因和影响,以获得土地覆被变化的年率和指数。分析工作是通过对环境、水和生态过渡部(MAATE)和军事地理研究所(IGM)网站上的地理信息进行地理处理,使用交叉表和重叠层的方法来确定既定日期之间的土地覆被对比。结果表明,在过去的 28 年中,农业用地和牧场分别累计增加了 17626.5 公顷和 16767.6 公顷;农业活动已成为森林砍伐的主要原因。1990-2018 年间,森林面积减少了 36 413 公顷,年森林砍伐率为 1 300 公顷*年-1。根据这一情况,我们可以估计,如果这一趋势持续下去,大约 30 年后,La Joya de los Sachas 的森林将完全消失。此外,在评估的三个时期(1990-2000 年、2000-2008 年和 2008-2018 年),公共政策、美元化、道路开通和农业活动的变化也对土地植被和土地利用产生了影响。关键词:土壤、土地利用、土地覆盖、农业用地、牧场、森林、地图。
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引用次数: 0
Ameliorative Effect of Cinnamon and Rosemary Oils in Acrylamide–Induced Hepatic Injury in Rats 肉桂和迷迭香油对丙烯酰胺诱导的大鼠肝损伤的改善作用
Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.58
Hala Elsayed, Ashraf Abd El-Hakim El komy, Elham Abd-El Moneim El-Shewy, Faten Ebrahim Elsayed Abdallah
Liver diseases can result from various causes, such as viruses, bacteria, autoimmune disorders, or certainmedications and toxic substances. While modern medicine offers treatments for these conditions, there needsto be more effective drugs that can protect and regenerate liver cells. Therefore, it is crucial to identify newtreatment options and liver-protective agents that are both highly efficient and safe. This study is assigned toinvestigate the adverse effects of acrylamide on the liver in rats and explore whether these effects can bemitigated by co-administration of cinnamon oil (C.O.), rosemary oil (R.O.), or a combination of both oilsduring acrylamide exposure. A total of 70 male albino rats were divided randomly into 7 groups, each groupof 10 rats, that received different treatments: control group, acrylamide-treated group (20 mg/kg b.wt), cinnamon oil-treated group (200 mg/kg b.wt), rosemary oil-treated group (250 mg/kg b.wt), acrylamide and cinnamon oil-treated group, acrylamide and rosemary oil-treated group, and acrylamide, cinnamon oil, and Rosemary oil-treated group. These treatments were administered orally for 28 consecutive days. Blood and livertissue samples were gathered at the end of the study to assess the outcomes. The results revealed that cinnamonoil and rosemary oils exhibited hepatoprotective effects, as evidenced by normalized liver function parameters(alanine transaminase, Aspartate transaminase, and Alkaline phosphatase), as well as improvements in nonenzymatic parameters (total protein, albumin, cholesterol, triglyceride, low-density lipoprotein, and high-density lipoprotein). The observed hepatoprotection of cinnamon oil and rosemary oils was attributed to theirability to reduce oxidative stress caused by acrylamide, as demonstrated by lower levels of liver cell lipidperoxidation product (malondialdehyde) and enhanced activity of antioxidative enzymes (glutathione and catalase) in liver tissue.Keywords: Cinnamon, Rosemary, Acrylamide, Liver, Rats, Antioxidants
肝脏疾病可由多种原因引起,如病毒、细菌、自身免疫失调或某些药物和有毒物质。虽然现代医学可以治疗这些疾病,但还需要更有效的药物来保护和再生肝细胞。因此,找到既高效又安全的新治疗方案和保肝药物至关重要。本研究旨在调查丙烯酰胺对大鼠肝脏的不良影响,并探讨在暴露于丙烯酰胺的过程中,同时服用肉桂油(C.O.)、迷迭香油(R.O.)或两种油的组合是否可以减轻这些影响。将 70 只雄性白化大鼠随机分为 7 组,每组 10 只,分别接受不同的处理方法:对照组、丙烯酰胺处理组(20 毫克/千克体重)、肉桂油处理组(200 毫克/千克体重)、迷迭香油处理组(200 毫克/千克体重)。重量)、迷迭香油处理组(250 毫克/千克体重)、丙烯酰胺和肉桂油处理组、丙烯酰胺和迷迭香油处理组以及丙烯酰胺、肉桂油和迷迭香油处理组。这些处理连续口服 28 天。研究结束时收集血液和活组织样本以评估结果。结果表明,肉桂油和迷迭香油具有保护肝脏的作用,肝功能参数(丙氨酸转氨酶、天门冬氨酸转氨酶和碱性磷酸酶)恢复正常,非酶参数(总蛋白、白蛋白、胆固醇、甘油三酯、低密度脂蛋白和高密度脂蛋白)也有所改善。肉桂油和迷迭香油对肝脏的保护作用可通过降低肝细胞脂质过氧化产物(丙二醛)的水平和提高肝组织中抗氧化酶(谷胱甘肽和过氧化氢酶)的活性来体现,这归因于它们能够减少丙烯酰胺引起的氧化应激:肉桂 迷迭香 丙烯酰胺 肝脏 大鼠 抗氧化剂
{"title":"Ameliorative Effect of Cinnamon and Rosemary Oils in Acrylamide–Induced Hepatic Injury in Rats","authors":"Hala Elsayed, Ashraf Abd El-Hakim El komy, Elham Abd-El Moneim El-Shewy, Faten Ebrahim Elsayed Abdallah","doi":"10.21931/rb/2024.09.01.58","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.58","url":null,"abstract":"Liver diseases can result from various causes, such as viruses, bacteria, autoimmune disorders, or certain\u0000medications and toxic substances. While modern medicine offers treatments for these conditions, there needs\u0000to be more effective drugs that can protect and regenerate liver cells. Therefore, it is crucial to identify new\u0000treatment options and liver-protective agents that are both highly efficient and safe. This study is assigned to\u0000investigate the adverse effects of acrylamide on the liver in rats and explore whether these effects can be\u0000mitigated by co-administration of cinnamon oil (C.O.), rosemary oil (R.O.), or a combination of both oils\u0000during acrylamide exposure. A total of 70 male albino rats were divided randomly into 7 groups, each group\u0000of 10 rats, that received different treatments: control group, acrylamide-treated group (20 mg/kg b.wt), cinnamon oil-treated group (200 mg/kg b.wt), rosemary oil-treated group (250 mg/kg b.wt), acrylamide and cinnamon oil-treated group, acrylamide and rosemary oil-treated group, and acrylamide, cinnamon oil, and Rosemary oil-treated group. These treatments were administered orally for 28 consecutive days. Blood and liver\u0000tissue samples were gathered at the end of the study to assess the outcomes. The results revealed that cinnamon\u0000oil and rosemary oils exhibited hepatoprotective effects, as evidenced by normalized liver function parameters\u0000(alanine transaminase, Aspartate transaminase, and Alkaline phosphatase), as well as improvements in nonenzymatic parameters (total protein, albumin, cholesterol, triglyceride, low-density lipoprotein, and high-density lipoprotein). The observed hepatoprotection of cinnamon oil and rosemary oils was attributed to their\u0000ability to reduce oxidative stress caused by acrylamide, as demonstrated by lower levels of liver cell lipid\u0000peroxidation product (malondialdehyde) and enhanced activity of antioxidative enzymes (glutathione and catalase) in liver tissue.\u0000Keywords: Cinnamon, Rosemary, Acrylamide, Liver, Rats, Antioxidants","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":"16 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140238640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro evaluation of the inhibitory capacity of three Trichoderma isolates on Ralstonia solanacearum 体外评估三种毛霉分离菌对茄枯镰孢菌的抑制能力
Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.6
Jimmy Trinidad Pico Rosado, Christopher Wilson Suárez Palacios, Jessenia Jiménez Cumbicus, Ernesto Paredes Puga, Gladys Sabando, Liliana Andrade Olalla
Bacterial wilt in bananas, caused by Ralstonia solanacearum or Moko, limits crop production and threatens Ecuador. This study evaluated Trichoderma isolates in laboratory conditions as an innovative alternative to ensure sustainability in banana production. The four R. solanacearum. isolates were obtained from banana plants exhibiting disease symptoms and were characterized through morphological and biochemical tests. Four treatments were evaluated: three isolates of fungi from the genus Trichoderma (Trichoderma viride, T. harzianum, T. asperellum) and one consisting of a combination of the three isolates above. The inhibitory capacity of the Trichoderma isolates on R. solanacearum colonies was measured. A completely randomized design with three replicates was used, and general linear and mixed models were employed, with qq-plot graphs for normality and residual plots for variance homogeneity. Furthermore, a Fisher's LSD test was conducted at a significance level of α = 0.05. In the biochemical tests, the bacterial isolates exhibited specific characteristics of R. solanacearum in two bacterial isolates. In the inhibition tests, treatment four and treatment one (consortium of the three Trichoderma isolates and Trichoderma viride) showed the highest inhibitory potential, with 76.07% and 61.19%, respectively. The consortium of Trichoderma isolates demonstrated the highest inhibitory potential against R. solanacearum, with day 10 being the time with the highest percentage of inhibition (72.61%). Keywords: Bacterial wilt, Ralstonia solanacearum, Trichoderma, inhibition
由 Ralstonia solanacearum 或 Moko 引起的香蕉细菌性枯萎病限制了作物产量,并威胁着厄瓜多尔。本研究评估了实验室条件下的毛霉菌分离株,作为确保香蕉生产可持续性的创新替代品。四种 R. solanacearum 分离物取自出现病害症状的香蕉植株,并通过形态学和生化测试进行鉴定。评估了四种处理方法:三种毛霉属真菌分离物(Trichoderma viride、T. harzianum、T. asperellum)和一种由上述三种分离物组合而成的处理方法。测定了毛霉菌分离物对茄碱菌菌落的抑制能力。采用了三个重复的完全随机设计,并使用了一般线性和混合模型,用 qq 图表示正态性,用残差图表示方差均匀性。此外,还进行了费雪 LSD 检验,显著性水平为 α = 0.05。在生化测试中,两种细菌分离物表现出茄碱菌的特异性。在抑制试验中,处理 4 和处理 1(三种毛霉分离物和病毒毛霉的联合体)的抑制潜力最高,分别为 76.07% 和 61.19%。三株毛霉的联合体对茄碱菌的抑制率最高,第 10 天的抑制率最高(72.61%)。关键词细菌枯萎病 Ralstonia solanacearum 毛霉 抑制作用
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引用次数: 0
Comparative Analysis of Threshold Cycle Results for RNA Extraction in SARS-CoV-2 RT-qPCR Using Magnetic Beads and Spin Column Methods 使用磁珠法和旋转柱法提取 SARS-CoV-2 RT-qPCR 中 RNA 的阈值循环结果比较分析
Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.64
Fardiah Tilawati Sitanggang, James Perdinan Simanjuntak, Nasrah Nasrah, Ridwansyah Ridwansyah, Arvida Bar
Coronavirus Disease-2019 (COVID-19) belongs to the large family of SARS-CoV viruses, initially emergingin 2002-2003. In humans, this virus triggers respiratory infectious diseases. COVID-19, a new variant ofSARS-CoV, was identified in humans following an unprecedented incident in Wuhan, China, in December2019. This virus typically manifests mild symptoms, including a runny nose, sore throat, cough, and fever.The Nucleic Acid Amplification Test (NAAT), specifically the realtime Reverse Transcription PolymeraseChain Reaction (rRT-PCR) examination, is recommended by the World Health Organization (WHO) for diagnosing COVID-19. This study assessed potential differences in Threshold Cycle results during RNA extraction using magnetic beads compared to spin columns in the SARS-CoV-2 rRT-PCR method. The population for this study was selected through accidental sampling from nasopharyngeal and oropharyngeal swabsof COVID-19 patients obtained between December 2022 and April 2023, with Threshold Cycle values<30,000. The samples were stored at -80°C. The findings revealed that the average N (VIC) was 23.359, andRdRP (FAM) was 25.558 in the Magnetic Beads method, indicating a lower value compared to the average N(VIC) of 29.200 and RdRP (FAM) of 29.661 in the Spin Column method. This suggests that the MagneticBeads method exhibited greater sensitivity than the Spin Column method. The statistical analysis confirmedthese differences, with a P value of 0.003 in N (VIC) and the P value of 0.000 in RdRP (FAM). Consequently,it can be concluded that there is a significant 19.5% difference in the Threshold Cycle during RNA extractionusing Magnetic Beads and Spin Column in the examination of the SARS-CoV-2 rRT-PCR method.Keywords: Sars-CoV-2; rRT-PCR; Magnetic Beads; Spin Column; Threshold Cycle.
冠状病毒病-2019(COVID-19)属于 SARS-CoV 病毒大家族,最初出现于 2002-2003 年。在人类中,这种病毒会引发呼吸道传染病。COVID-19 是 SARS-CoV 的一个新变种,于 2019 年 12 月在中国武汉发生的一起史无前例的事件中在人类中被发现。世界卫生组织(WHO)推荐使用核酸扩增检验(NAAT),特别是实时反转录聚合酶链反应(rRT-PCR)检查来诊断 COVID-19。本研究评估了在 SARS-CoV-2 rRT-PCR 方法中使用磁珠提取 RNA 与使用旋柱提取 RNA 时阈值周期结果的潜在差异。本研究的样本选自 2022 年 12 月至 2023 年 4 月间意外采集的 COVID-19 患者的鼻咽和口咽拭子,阈值周期值<30,000。样本在-80°C下保存。研究结果显示,磁珠法的平均 N (VIC) 为 23.359,RdRP (FAM) 为 25.558,与旋转柱法的平均 N (VIC) 29.200 和 RdRP (FAM) 29.661 相比,数值较低。这表明磁珠法比自旋色谱柱法具有更高的灵敏度。统计分析证实了这些差异,N (VIC) 的 P 值为 0.003,RdRP (FAM) 的 P 值为 0.000。因此,可以得出结论:在 SARS-CoV-2 rRT-PCR 方法检测中,磁珠法和旋转柱法提取 RNA 的阈值周期存在 19.5%的显著差异:SARS-CoV-2;rRT-PCR;磁珠;旋光柱;阈值周期。
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Bionatura
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