Histopathological effects of Cryptococcus neoformans on liver and kidney in mice

This study provides a brief review of approaches for the detection of histopathological effects of Cryptococcus neoformans on the liver and kidney in mice that were injected I/P with 105 yeast cells of C. neoformans suspended in 1 ml phosphate-buffered saline at a single dose. After 14 days, the mice were sacrificed, and histopathological sections from the liver and kidney were prepared and stained with Haematoxylin and Eosin by the P.A.S. method. The results show that the liver was infiltrated with inflammatory cells, primarily mononuclear cells, in the portal. In addition to the activation of Kupffer cells and vacillation of hepatocytes, most blood vessels were congested. The section of the kidney shows sluffing of epithelia lining tubules and destruction of glomeruli, in addition to infiltration of mononuclear cells. These results suggested that the fungus invasiveness of mice has substantial effects on vital organs and may lead to death. Keywords: Cryptococcus neoformans, Hepatic cryptococcal infection, Cryptococcus