Production and investigation of bio-textile films produced from bacterial cellulose biosynthesis from black tea and ginger, and cultivation on sugar cane media

In this study, bacterial cellulose was produced through the fermentation of a mixture of black tea, ginger, and sugar, and used to create bio-textile films on sugarcane-based media. Characterization included ribosomal RNA gene sequencing, FTIR spectroscopy, XRD, and SEM was used to examine surface morphology. The bio-textile films showed increasing UV resistance beyond 10 days of cultivation (T.UVA%; 0.13 ± 0.02, T.UVB%; 0.22 ± 0.01, UPF; 629 ± 2.12) and antimicrobial resistance was assessed by quantifying Colony-Forming Units (CFU), resulting in a 100% reduction in growth for both Escherichia coli and Staphylococcus aureus. Subsequently, after 15 days of cultivation, antimicrobial activity was evaluated using the disc agar diffusion method, yielding noteworthy outcomes. E. coli displayed a 25 mm zone of inhibition, S. aureus exhibited a 31 mm zone of inhibition, Candida albicans showed a 35 mm zone of inhibition, and Aspergillus niger presented a 22 mm zone of inhibition.