梭菌菌株 BOH3 从粗甘油中生产出大量 1,3-丙二醇:接种物和底物浓度的关键作用

IF 2.8 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of chemical technology and biotechnology Pub Date : 2024-03-27 DOI:10.1002/jctb.7643
Gobinath Rajagopalan, Akanksha Jain, Venkata Giridhar Poosarla, Chandraraj Krishnan, Kun Lin Yang
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引用次数: 0

摘要

1,3-丙二醇(PDO)是一种原料/特种化学品/添加剂,可应用于多种工业领域。一种新的野生型梭菌菌株 BOH3 可以利用粗甘油(CG)生产 PDO。这项研究揭示了其利用粗甘油生产 PDO 的潜力。
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High level of 1,3-propanediol production from crude glycerol by Clostridium strain BOH3: critical roles of inoculum and substrate concentration

BACKGROUND

1,3-Propanediol (PDO) is a raw material/specialty chemical/additive in several industrial applications. A new wild-type Clostridium strain BOH3 can utilize crude glycerol (CG) and produce PDO. This investigation reveals its potential for PDO production from CG.

RESULTS

Initially, inoculum of strain BOH3 was cultured from reinforced clostridial medium (RCM) with glucose (medium-A), and modified-RCM with glycerol (medium-B). While fermenting 20 g L−1 glycerol, the cells cultivated from medium-A produced 5.75 g L−1 PDO (as major product) with 3.10 g L−1 butanol (byproduct), whereas the cells cultivated from medium-B produced 11.01 g L−1 PDO with 2.50 g L−1 butyric acid and 1.01 g L−1 acetic acid, with no butanol produced. To enhance PDO production from the batch-process, various parameters including pure glycerol/CG concentration (80 g L−1), inoculum age (15 h) and size (12.50%v/v) of cells from medium-B, initial medium-pH (pH 6.4) and incubation temperature (39 °C) were studied; the optimal values obtained are given in their respective parenthesis. Under optimal conditions, PDO production was 42.58–47.15 g L−1 (0.65–0.79 mol-PDO/mol-glycerol) from CG-based rich/mineral media. Furthermore, fed-batch fermentation with 50–80 g L−1 initial CG-concentration was tested to enhance the PDO production. Among these, 60 g L−1 initial CG-concentration assisted strain BOH3 in utilizing a high level of CG (~174 g L−1); it supported a higher-titer of PDO production (103.55–116.45 g L−1), with the highest yield (0.72–0.81 mol−PDO/mol−glycerol) ever reported.

CONCLUSION

Clostridium strain BOH3 shows the potential to support a high titer (>116 g L−1) with the highest yield (>0.80 mol mol−1) of PDO production from CG (a waste). It can be considered for industrial-scale PDO production. © 2024 Society of Chemical Industry (SCI).

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来源期刊
CiteScore
7.00
自引率
5.90%
发文量
268
审稿时长
1.7 months
期刊介绍: Journal of Chemical Technology and Biotechnology(JCTB) is an international, inter-disciplinary peer-reviewed journal concerned with the application of scientific discoveries and advancements in chemical and biological technology that aim towards economically and environmentally sustainable industrial processes.
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Issue Information Adsorption behavior of graphite‐like walnut shell biochar modified with ammonia for ciprofloxacin in aqueous solution Eco‐friendly approaches for synthesis of indolyl 1H‐pyrroles using rice‐husk‐derived carbonaceous sulfonation as the green catalyst Impact of neutrophil‐activating protein conservation on diagnostic tests and vaccine design Issue Information
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